From AureoWiki
Jump to: navigation, search
Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1676
  • pan locus tag?: SAUPAN004486000
  • symbol: NWMN_1676
  • pan gene symbol?:
  • synonym:
  • product: putative transposase for IS1272

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1676
  • symbol: NWMN_1676
  • product: putative transposase for IS1272
  • replicon: chromosome
  • strand: +
  • coordinates: 1870802..1871626
  • length: 825
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGATGTTAAAAATCATCTTATATGCATATACTCAATCTGTATTTTCTGGTCGAAGAATA
    GAGAAATTACTTCATGACAGTATTCGAATGATGTGGTTAGCTCAAAATCAAACACCTTCT
    TATAAAACTATTAATCGTTTTAGAGTGAATCCTAATACTGATGCGTTAATTGAATCTTTA
    TTTATTCAGTTCCATAGTCAATGTTTAAAGCAAAATCTTATTGATAATAATTCAATTTTT
    ATTGATGGTACAAAAGTAGAAGCTAATGCCAATAGATATACATTTGTGTGGAAGAAAAGT
    ATTCAAAATCACGAATCGAAATTGAACGAAAATTCAAAAGCATTATATCGTGACTTAGTT
    GAAGAAAAAATAATACCAGAGATAAAAGAAGATGGAGATAGCGATTTAACAATAGAAGAA
    ATAGATTTAATTGGTAGTCATTTAGATAAAGAAATCGAAGATTTAAATCATTCTATTGAG
    AACGAAGATTGTGCTCAAATTAGAAAACAGACCCGTAAAAAAAGAACTGAGATTAAGAAG
    TTCAAAAAGAAATTTGATGATTATTCCGAAAGAAAAAATAAATATGAAGAACAAAAATCG
    ATTCTTAAAGATAGAAATAGTTTTTCTAAAACTGATCATGATGCAACTTTTATGAGAATG
    AAGGAAGACCATATGAAAAATGGCCAACTTAAGCCAGGATACAATTTACAAATAGCGACA
    AATTCTCAATTTGTTTTATCCTATGACCTATTTCAAAACCCGACAGATACTAGAACTTTA
    ATTCCATTTTTAACAATGATTCAAAATACCTTCGGTTATTTGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    825

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1676
  • symbol: NWMN_1676
  • description: putative transposase for IS1272
  • length: 274
  • theoretical pI: 8.5855
  • theoretical MW: 32410.6
  • GRAVY: -0.767883

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    IS1272-like transposase, degenerate 
  • PFAM:
    no clan definedDUF772; Transposase domain (DUF772) (PF05598; HMM-score: 45.9)
    RNase_H (CL0219) DDE_Tnp_1; Transposase DDE domain (PF01609; HMM-score: 32.2)
    Toprim-like (CL0413) DUF2220; Uncharacterized protein conserved in bacteria C-term(DUF2220) (PF09983; HMM-score: 13.2)
    no clan definedPCRF; PCRF domain (PF03462; HMM-score: 11)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.138
    • Ymax_pos: 11
    • Cmax: 0.114
    • Cmax_pos: 43
    • Smax: 0.248
    • Smax_pos: 2
    • Smean: 0.192
    • D: 0.159
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MMLKIILYAYTQSVFSGRRIEKLLHDSIRMMWLAQNQTPSYKTINRFRVNPNTDALIESLFIQFHSQCLKQNLIDNNSIFIDGTKVEANANRYTFVWKKSIQNHESKLNENSKALYRDLVEEKIIPEIKEDGDSDLTIEEIDLIGSHLDKEIEDLNHSIENEDCAQIRKQTRKKRTEIKKFKKKFDDYSERKNKYEEQKSILKDRNSFSKTDHDATFMRMKEDHMKNGQLKPGYNLQIATNSQFVLSYDLFQNPTDTRTLIPFLTMIQNTFGYL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]