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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1701 [new locus tag: NWMN_RS09560 ]
  • pan locus tag?: SAUPAN004558000
  • symbol: splF
  • pan gene symbol?: splF
  • synonym:
  • product: serine protease SplF

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1701 [new locus tag: NWMN_RS09560 ]
  • symbol: splF
  • product: serine protease SplF
  • replicon: chromosome
  • strand: -
  • coordinates: 1899285..1900004
  • length: 720
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAAAAATATAATCATCAAAAGTATTGCAGCATTGACGATTTTAACATCAATAACT
    GGTGTCGGCACAACAATGGTTGAAGGTATTCAACAAACAGCCAAAGCCGAAAATACTGTT
    AAACAAATTACAAATACAAATGTTGCACCATACAGTGGTGTTACATGGATGGGCGCTGGA
    ACAGGATTTGTAGTTGGAAATCATACAATCATTACCAATAAACATGTTACCTATCACATG
    AAAGTCGGTGATGAAATCAAAGCACATCCTAATGGTTTTTATAATAACGGTGGTGGACTT
    TATAAAGTTACTAAGATTGTAGATTATCCTGGTAAAGAAGATATTGCGGTTGTACAAGTT
    GAAGAAAAATCAACACAACCAAAAGGTAGAAAATTCAAAGATTTCACTAGTAAATTTAAT
    ATAGCATCAGAAGCTAAAGAAAATGAACCTATATCAGTCATTGGTTATCCAAATCCTAAT
    GGAAATAAACTACAAATGTATGAATCAACTGGTAAAGTATTATCAGTGAATGGGAATATA
    GTGTCATCGGATGCAATTATTCAGCCTGGTAGCTCTGGTTCACCTATATTAAATAGTAAA
    CACGAAGCTATTGGTGTAATCTATGCCGGTAATAAGCCATCAGGTGAAAGCACAAGAGGA
    TTTGCTGTTTATTTCTCTCCTGAAATTAAGAAATTCATTGCAGATAATTTAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1701 [new locus tag: NWMN_RS09560 ]
  • symbol: SplF
  • description: serine protease SplF
  • length: 239
  • theoretical pI: 9.6769
  • theoretical MW: 25655
  • GRAVY: -0.291213

Function[edit source | edit]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 24.9)
    Genetic information processingProtein fateProtein folding and stabilizationpeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 24.9)
  • TheSEED:  
    serine protease 
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 62.5)
    Trypsin; Trypsin (PF00089; HMM-score: 58.9)
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 24.2)
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 16.9)
    Peptidase_S29; Hepatitis C virus NS3 protease (PF02907; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide length 37 aa
    • Ymax: 0.522
    • Ymax_pos: 37
    • Cmax: 0.457
    • Cmax_pos: 37
    • Smax: 0.835
    • Smax_pos: 11
    • Smean: 0.69
    • D: 0.588
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQITNTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPNGNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGESTRGFAVYFSPEIKKFIADNLDK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]