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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1725 [new locus tag: NWMN_RS09725 ]
  • pan locus tag?: SAUPAN004758000
  • symbol: hemE
  • pan gene symbol?: hemE
  • synonym:
  • product: uroporphyrinogen decarboxylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1725 [new locus tag: NWMN_RS09725 ]
  • symbol: hemE
  • product: uroporphyrinogen decarboxylase
  • replicon: chromosome
  • strand: -
  • coordinates: 1925657..1926694
  • length: 1038
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTGCATAATAAAAACAATACAATTTTAAAAATGATCAAAGGTGAAGAAACATCACAT
    ACACCTGTTTGGTTTATGCGACAAGCTGGCCGTTCGCAACCAGAATATCGAAAATTGAAA
    GAAAAATATTCACTATTCGATATTACACATCAGCCGGAGTTGTGCGCTTATGTAACACAT
    TTACCAGTTGATAATTATCATACAGATGCAGCAATTTTATACAAAGATATTATGACACCA
    TTAAAGCCAATTGGTGTCGATGTAGAAATTAAATCGGGTATTGGTCCAGTGATTCATAAT
    CCAATCAAAACAATTCAAGATGTTGAGAAACTTTCTCAAATAGACCCCGAACGAGATGTA
    CCATATGTATTAGATACAATTAAACTTTTAACAGAAGAAAAGTTAAATGTGCCGCTAATA
    GGATTTACTGGGGCACCATTTACATTAGCGTCATATATGATTGAAGGCGGACCATCGAAA
    AATTACAATTTTACAAAAGCGATGATGTATAGAGATGAAGCAACATGGTTTGCTTTAATG
    AATCATTTAGTTGATGTATCTGTTAAATATGTAACAGCTCAAGTCGAAGCAGGTGCCGAA
    TTGATTCAAATTTTCGATTCATGGGTAGGTGCATTAAATGTCGAGGATTACAGACGTTAC
    ATTAAACCACATATGATTCGATTAATCAGTGAGGTTAAAGAAAAACATGATGTGCCGGTA
    ATTTTATTCGGTGTAGGTGCCAGTCATTTAATCAATGAATGGAATGATTTACCGATTGAT
    GTATTAGGCCTAGATTGGAGAACGTCTATTAATCAGGCTCAACAATTAGGCGTTACTAAA
    ACATTACAAGGGAATTTAGATCCATCAATTTTATTAGCACCATGGAATGTCATTGAAGAG
    AGATTGAAACCAATATTAGATCAAGGTATGGAGAACGGTAAACACATCTTTAATTTAGGA
    CACGGTGTATTCCCAGAAGTGCAACCAGAGACGTTACGTAAAGTAAGCGAATTCGTACAC
    ACATATACACAAAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1038

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1725 [new locus tag: NWMN_RS09725 ]
  • symbol: HemE
  • description: uroporphyrinogen decarboxylase
  • length: 345
  • theoretical pI: 6.324
  • theoretical MW: 39352.1
  • GRAVY: -0.231304

Function[edit source | edit]

  • reaction:
    EC 4.1.1.37?  ExPASy
    Uroporphyrinogen decarboxylaseUroporphyrinogen III = coproporphyrinogen + 4 CO2
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminuroporphyrinogen decarboxylase (TIGR01464; EC 4.1.1.37; HMM-score: 420.6)
    methyltransferase, MtaA/CmuA family (TIGR01463; EC 2.1.1.-; HMM-score: 61.8)
    Signal transductionRegulatory functionsProtein interactionsphosphohistidine phosphatase SixA (TIGR00249; EC 3.1.3.-; HMM-score: 15.4)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsTetrapyrrolesCPO analysis Uroporphyrinogen III decarboxylase (EC 4.1.1.37) 
  • PFAM:
    Methionine_synt (CL0160) URO-D; Uroporphyrinogen decarboxylase (URO-D) (PF01208; HMM-score: 403.6)
    TPR (CL0020) COPI_C; Coatomer (COPI) alpha subunit C-terminus (PF06957; HMM-score: 11.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.164
    • Ymax_pos: 12
    • Cmax: 0.117
    • Cmax_pos: 40
    • Smax: 0.373
    • Smax_pos: 5
    • Smean: 0.265
    • D: 0.203
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYSLFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPIKTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSKNYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYRRYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQLGVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRKVSEFVHTYTQR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: NWMN_1753 (PerR*)
    PerR* (TF) important in Oxidative stress responseRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]