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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1821 [new locus tag: NWMN_RS10460 ]
  • pan locus tag?: SAUPAN004898000
  • symbol: NWMN_1821
  • pan gene symbol?:
  • synonym:
  • product: ribonuclease BN

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1821 [new locus tag: NWMN_RS10460 ]
  • symbol: NWMN_1821
  • product: ribonuclease BN
  • replicon: chromosome
  • strand: +
  • coordinates: 2027873..2029090
  • length: 1218
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTCGAAAAAGGATCACTCTTCTTCAAAATACCTTAATTCTGTTAAGGAAGCGCAAGAG
    GAGTCAAAAAAGAAAAATAAAAGTAATCCCAAAATTGATGTTGATCGTACATATATTGAA
    CCTCAACAATTCCAATCTAAGAAACCTAAAAAAGATGATCAGGTTTTCTTCTTATCAAGA
    TTAAATAAACCTGCAAAATATAAGAAAGACTCTAATTTCTTATCATATCTCATCTATCGC
    ATAGGAAAAGATGATGCCTCAGGACTAGCAGCACAAATGACTTACCATTTCGTACTTGCT
    ATGTTCCCTATGTTGCTTTTCCTATTAACATTATTACCATTTTTCAATATTAAGCAGAGT
    CAAATTACTAATATGTTAAGCAATGCACCCGCTGAAACATCTACTCTAATTAAGAGTGTA
    ATTGGTGATATAACTCAAAACTCCAGTGGTGGCTTATTATCTATCGGTTTGATTTTAGCA
    ATTTGGTCAGCTTCAAATGGAATGACTGCAATTATGAATTCTTTCAATGTTGCTTACGAT
    GTAGAAGATAGCCGTAATGGAATCGTATTAAAACTACTAAGTGTTGTCTTCACTGTAGTT
    ATGGGCGTTGTGTTTGTAGTTGCTCTAGCATTACCAACGCTTGGTTCTGTAATTAGTCAT
    TTCCTATTCGGTCCACTTGGATTTGACGAACAAGTGAAATGGATTTTTAACCTTATTAGA
    ATTGTGTTACCAATCATTATTATATTTATCATATTTATCGTGTTATATTCGGTTGCACCT
    AACGTTAAAACGAAGCTTAAGTCAGTATTACCAGGTGCAGTATTTACTTCAATTATTTGG
    TTAGCTGGTTCATTTGGTTTTGGTTGGTATATTTCAAATTTTGGTAACTATTCTAAAACA
    TATGGCAGTATCGCGGGTATCATCATTTTGTTACTATGGTTATATATCACAAGTTTTATT
    ATAATTGTCGGTGCTGAAATCAATGCAATCATTCATCAGCGTAGTGTAATTAAAGGTAAA
    ACACCTGAAGAAGCAGCATTAGAACATGATGACAATAATAAAAATCATTATAATGAGAAC
    ACTAGATACGAATATGATGAAGATAGTAATGCAACACATCAACGTACGTATCATGTTGAT
    GAACATCCTAGTGATACCTATCCAGAAGATGATAAAAATATAAAAGACAAAATCGTTGAT
    AAGCTTAAAAAAGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1218

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1821 [new locus tag: NWMN_RS10460 ]
  • symbol: NWMN_1821
  • description: ribonuclease BN
  • length: 405
  • theoretical pI: 9.26901
  • theoretical MW: 45604.4
  • GRAVY: 0.0162963

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionGeneralYihY family inner membrane protein (TIGR00765; HMM-score: 165.5)
    Genetic information processingTranscriptionDegradation of RNAinner membrane protein YhjD (TIGR00766; HMM-score: 33.6)
  • TheSEED:  
    RNA MetabolismRNA processing and modificationtRNA processing Ribonuclease BN (EC 3.1.-.-) 
    CBSS-176280.1.peg.1561 Ribonuclease BN (EC 3.1.-.-) 
    CBSS-342610.3.peg.283 Ribonuclease BN (EC 3.1.-.-) 
    LMPTP YfkJ cluster Ribonuclease BN (EC 3.1.-.-) 
  • PFAM:
    no clan definedVirul_fac_BrkB; Virulence factor BrkB (PF03631; HMM-score: 234.3)
    DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 11.5)
    Oxa1 (CL0376) 60KD_IMP; 60Kd inner membrane protein (PF02096; HMM-score: 10.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.166
    • Ymax_pos: 21
    • Cmax: 0.11
    • Cmax_pos: 21
    • Smax: 0.404
    • Smax_pos: 2
    • Smean: 0.265
    • D: 0.205
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQFQSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMFPMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILAIWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVISHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFTSIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQRSVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDKNIKDKIVDKLKKD

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SigB [1] [2] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J. Bacteriol.: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)
  2. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]