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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1995 [new locus tag: NWMN_RS11545 ]
  • pan locus tag?: SAUPAN005373000
  • symbol: thiE
  • pan gene symbol?: thiE
  • synonym:
  • product: thiamine-phosphate pyrophosphorylase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1995 [new locus tag: NWMN_RS11545 ]
  • symbol: thiE
  • product: thiamine-phosphate pyrophosphorylase
  • replicon: chromosome
  • strand: -
  • coordinates: 2216965..2217606
  • length: 642
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTTAACCAATCGTATCTAAATGTGTATTTTATTTGTGGGACCTCCGATGTTCCGAGT
    CATCGAACTATTCATGAAGTATTAGAAGCAGCATTAAAGGCAGGGATTACACTTTTCCAA
    TTTCGAGAAAAGGGTGAATCAGCTTTAAAAGGAAACGATAAACTTGTATTAGCTAAAGAA
    TTACAGCATCTCTGTCATCAATATGATGTGCCTTTTATCGTTAATGATGATGTATCCTTG
    GCAAAAGAAATTAACGCGGATGGTATACATGTCGGTCAAGATGATGCGAAAGTCAAAGAG
    ATAGCACAATATTTCACTGATAAAATTATTGGACTTAGTATTAGTGATTTAGATGAGTAT
    GCAAAATCTGATTTAACTCATGTTGATTATATAGGTGTTGGACCAATCTATCCTACGCCG
    TCGAAGCATGACGCGCATATACCAGTAGGTCCAGAAATGATTGCAACGTTTAAAGAAATG
    AATCCGCAACTACCGATTGTAGCTATTGGCGGTATCAATACTAATAATGTGGCGCCGATT
    GTAGAAGCAGGTGCCAATGGAATTTCAGTCATTTCAGCCATTTCTAAAAGTGAAAATATT
    GAAAAGACTGTTAATCGATTCAAAGATTTTTTTAATAATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    642

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1995 [new locus tag: NWMN_RS11545 ]
  • symbol: ThiE
  • description: thiamine-phosphate pyrophosphorylase
  • length: 213
  • theoretical pI: 5.06131
  • theoretical MW: 23398.4
  • GRAVY: -0.110798

Function[edit | edit source]

  • reaction:
    EC 2.5.1.3?  ExPASy
    Thiamine phosphate synthase 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2 4-amino-2-methyl-5-diphosphomethylpyrimidine + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 211.9)
    and 6 more
    Metabolism Energy metabolism Pentose phosphate pathway ribulose-phosphate 3-epimerase (TIGR01163; EC 5.1.3.1; HMM-score: 20.4)
    phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 18)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, synthase subunit Pdx1 (TIGR00343; HMM-score: 15.2)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 13.7)
    3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 12.8)
    Genetic information processing Protein synthesis tRNA aminoacylation O-phosphoseryl-tRNA(Sec) selenium transferase (TIGR03531; EC 2.9.1.2; HMM-score: 11)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 199.1)
    and 8 more
    DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 24.4)
    ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 19.3)
    PLP_aminotran (CL0061) SepSecS; O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS (PF05889; HMM-score: 18.4)
    TIM_barrel (CL0036) NanE; Putative N-acetylmannosamine-6-phosphate epimerase (PF04131; HMM-score: 15.9)
    Ribul_P_3_epim; Ribulose-phosphate 3 epimerase family (PF00834; HMM-score: 13.8)
    DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 13.6)
    QRPTase_C; Quinolinate phosphoribosyl transferase, C-terminal domain (PF01729; HMM-score: 12.9)
    Peptidase_CA (CL0125) Peptidase_C78; Peptidase family C78 (PF07910; HMM-score: 12.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005081
    • TAT(Tat/SPI): 0.000633
    • LIPO(Sec/SPII): 0.001509
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEMNPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: THI-box (transcription termination) regulon
    THI-box(RNA)important in Thiamine biosynthesis; transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]