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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2060 [new locus tag: NWMN_RS11895 ]
  • pan locus tag?: SAUPAN005500000
  • symbol: mtlD
  • pan gene symbol?: mtlD
  • synonym:
  • product: mannitol-1-phosphate 5-dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2060 [new locus tag: NWMN_RS11895 ]
  • symbol: mtlD
  • product: mannitol-1-phosphate 5-dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 2281347..2282453
  • length: 1107
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAAGCAGTTCACTTTGGTGCTGGTAACATAGGTCGTGGTTTCATTGGTTATATTCTT
    GCAGACAACAATGTTAAAGTAACATTTGCAGACGTCAATGAAGAAATCATTAATGCTTTA
    GCTCATGATCATCAATACGATGTTATTTTAGCTGATGAGTCTAAAACAACGACGCGCGTG
    AATAATGTTGATGCAATTAATTCAATGCAACCTTCTGAAGCGTTGAAACAAGCAATTCTA
    GAAGCTGATATTATTACAACAGCTGTTGGTGTTAACATACTACCTATTATTGCTAAATCT
    TTTGCGCCTTTCTTAAAAGAAAAAACAAACCATGTTAATATTGTTGCTTGTGAGAATGCT
    ATTATGGCAACTGATACATTGAAAAAAGCAGTACTTGATATTACTGGCCCTCTTGGTAAC
    AATATTCATTTTGCTAACTCAGCAGTTGATAGAATTGTACCATTACAAAAGAATGAAAAT
    ATATTAGACGTTATGGTTGAGCCATTTTACGAATGGGTTGTTGAAAAAGATGCATGGTAT
    GGTCCAGAACTAAACCATATTAAATATGTTGATGATTTAACACCATATATTGAGCGTAAA
    TTATTAACTGTGAATACAGGACATGCATATTTAGCGTATGCTGGTAAATTTGCAGGTAAA
    GCTACAGTTTTAGATGCAGTTGAAGATAGTTCAATTGAAGCTGGCTTACGCCGTGTTTTA
    GCTGAAACTAGTCAATATATTACTAATGAATTTGATTTTACTGAAGCGGAACAAGCTGCT
    TATGTTGAAAAAATAATAGATCGTTTCAACAATTCTTATTTATCTGATGAAGTAACACGT
    GTCGGACGAGGTACATTACGTAAAATTGGCCCTAAAGATAGAATTATAAAACCATTAACA
    TATCTTTATAATAAAGATTTAGAACGCACTGGTTTATTAAATACAGCTGCATTGTTATTG
    AAGTATGATGATACAGCAGACCAAGAAACTGTTGAGAAAAATAATTACATTAAAGAACAC
    GGTTTAAAAGCGTTTTTAAGTGAATATGCTAAAGTTGACGATGGCTTAGCCGATGAAATA
    ATTGAAGCGTACAATTCACTTTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1107

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2060 [new locus tag: NWMN_RS11895 ]
  • symbol: MtlD
  • description: mannitol-1-phosphate 5-dehydrogenase
  • length: 368
  • theoretical pI: 4.66047
  • theoretical MW: 40951
  • GRAVY: -0.171467

Function[edit source | edit]

  • reaction:
    EC 1.1.1.17?  ExPASy
    Mannitol-1-phosphate 5-dehydrogenaseD-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH
  • TIGRFAM:
  • TheSEED:  
    CarbohydratesSugar alcoholsMannitol Utilization Mannitol-1-phosphate 5-dehydrogenase (EC 1.1.1.17) 
  • PFAM:
    6PGD_C (CL0106) Mannitol_dh_C; Mannitol dehydrogenase C-terminal domain (PF08125; HMM-score: 144.5)
    NADP_Rossmann (CL0063) Mannitol_dh; Mannitol dehydrogenase Rossmann domain (PF01232; HMM-score: 71.2)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 20.8)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 18.6)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 18.4)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 16.1)
    Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 13.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.196
    • Ymax_pos: 31
    • Cmax: 0.21
    • Cmax_pos: 31
    • Smax: 0.267
    • Smax_pos: 18
    • Smean: 0.141
    • D: 0.175
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDHQYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFAPFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNENILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFAGKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSDEVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKNNYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SigB [1] [2] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator: NWMN_2058 (MtlR*)
    MtlR* (TF) important in Mannitol utilization  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]