From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2227 [new locus tag: NWMN_RS12840 ]
  • pan locus tag?: SAUPAN005807000
  • symbol: NWMN_2227
  • pan gene symbol?:
  • synonym:
  • product: Na+/H+ antiporter family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2227 [new locus tag: NWMN_RS12840 ]
  • symbol: NWMN_2227
  • product: Na+/H+ antiporter family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2447898..2449277
  • length: 1380
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAAACCTCTTTCTTCATCGTTTGTGAGAAAGAGGTATTTTTAATTGGAAAGCAGGTA
    AAAAGGATGGAAGTACATAAAAAGAGCAATGCTTGGGCATTATTCCCCTTGTTATTATTT
    GTGGCGTTGTTTTTAGGCGTAGGTATTATCACAGGTGATTTTACTTCAATGCCATTAAAT
    GTTGCAATTACGATAACGGTAATTGTGGCGTTATTAATGAATCGAAAAGAATCATTTGCT
    AAAAAAGTTGAGGTCTTCACGAAAGGTGCAGGCCATTCAAACATTGTTTTAATGATGTTG
    ATTTTTATTTTAGCAGGTGCATTTTCAAATACAGCTGAAAAAATGGGTGGCGTAAAGTCG
    ACAGTTAATTTAGGACTATCATTAATCCCAGAAAATTTAATTATTGTAGGATTGTTTGTG
    ATTTGTATGTTTGTTTCTATATCTATGGGGACGTCTGTAGGAACTGTTGCTGCAATAGCA
    CCTGTTGGTTACGGATTTGCACAAGCGACAGATGTACCAACTGCGCTAGCAATGGCTACA
    GTTGTTGGAGGTGCAATGTTCGGCGATAATCTATCAATGATTTCAGATACAACAATTGCT
    GCGGTTAGAACACAACATACGAAAATGAAAGATAAATTCAGAGTAAACTTTAAAATTGTA
    TTGCCAGGTGCTATTTTAACGATTATTATTCTGTATTTCTTAACGAATGGCATTTCTTTA
    AATCATGCTAAAAACTATGATTATAATCTAATTAAAGTTGTACCGTATATCTTAGTATTG
    GTATTAGCATTATTAGGTGTAAATGTCATTATCGTATTGATTGGCGGAACATTATTAGCC
    GGAATCATTGGTCTCATAGATGGTTCATTCGGTTGGATGGGGCTACTAGATGCAGTGTCC
    AAAGGTATTATAAGCATGGAAGATATTGCTATGATTGCGTTGCTAATTGGTGGTTTAGTC
    GGTATTATTCAACACAATGGTGGTATTGAATGGTTGTTGCAATTTGTTCGCTCTAAAGTG
    AAATCAAAGCGTGGCGCAGAATTAGGAATTGCTAGTTTGGTAAGTGTCGCAGATATTGCC
    ACGGCAAACAACACCATCTCTATTATTATGTCAGGTCCTTTAGCTAAAAATATTGCAGAT
    GAATATGATGTAGATTCAAGGAAATCAGCAAGTATTCTTGATATATTTGGAGGCTGCTTC
    CAAGGTTTATTACCATATAGCCCGCAAGTCATTTCAGCGGCTGGTGTAGCTGGTATTTCA
    CCGTTAATGTTATTCCCATACAGCATTTATCCTATATTATTAGGTGTATGCGGACTTATT
    GCGATTTTATTTAATTTTCCAAAGCTAAAAAAGAACACTAGTCAAGAAATGAAACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2227 [new locus tag: NWMN_RS12840 ]
  • symbol: NWMN_2227
  • description: Na+/H+ antiporter family protein
  • length: 459
  • theoretical pI: 9.66495
  • theoretical MW: 48937.3
  • GRAVY: 0.820261

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsNa+/H+ antiporter NhaC (TIGR00931; HMM-score: 86.8)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineMethionine Biosynthesis Methionine transporter MetT 
    Lysine, threonine, methionine, and cysteineMethionine Degradation Methionine transporter MetT 
  • PFAM:
    IT (CL0182) Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 80.4)
    Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 21.1)
    DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 18.8)
    CitMHS; Citrate transporter (PF03600; HMM-score: 15.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.151
    • Ymax_pos: 23
    • Cmax: 0.144
    • Cmax_pos: 23
    • Smax: 0.232
    • Smax_pos: 1
    • Smean: 0.146
    • D: 0.149
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKTSFFIVCEKEVFLIGKQVKRMEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITITVIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNLGLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVVGGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISLNHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSVADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAAGVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]