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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2246 [new locus tag: NWMN_RS12935 ]
  • pan locus tag?: SAUPAN005843000
  • symbol: NWMN_2246
  • pan gene symbol?: gltS
  • synonym:
  • product: sodium/glutamate symporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2246 [new locus tag: NWMN_RS12935 ]
  • symbol: NWMN_2246
  • product: sodium/glutamate symporter
  • replicon: chromosome
  • strand: +
  • coordinates: 2468280..2469488
  • length: 1209
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGATAGAACTTAATGCAATTACAACATTATGTTTAGCTTGTATCCTTTATTTACTTGGT
    AAGGCTATCGTTAATCACGTTAATTTTTTAAAACGTATTTGTATACCAGCACCAGTGATT
    GGCGGCTTAATCTTTGCTATTTTAGTTGCGGCTTTGGATTCATTTGGCATGGTTAAGATT
    AAATTAGATGCTTCATTCATTCAAGATTTCTTCATGTTAGCATTCTTTACGACAATCGGT
    CTTGGTGCATCATTGAAATTATTTAAATTAGGTGGCAAAGTCTTGCTATTATACTTTATG
    TTTTGTGCTATCATTTCAGTCATTCAAAACATAGTTGGTGTATCACTAGCAAAAGTATTA
    AATATTAAACCTTTGTTAGGATTAACAGCAGGTTCCATGTCTATGGAAGGCGGTCATGGT
    AATGCTGCTGCTTATGGTAAGACAATTCAAGATTTAGGTATTGATTCGGCACTGACAGCG
    GCTCTTGCAGCTGCAACTTTAGGTCTTGTATTTGGAGGGCTTATCGGTGGTCCAGTTGTA
    AAATTTTTAATCAAACGTTATAACTTAAAGCCTCAACATAGTGACGATACATTTAAAGAT
    TATAGCCAAGTAGCATATAACGAACATTTACATAGTAAATTTAATGCCACTGAAGTATTC
    TTCATTCAATTTACAATCGTTGTATTCTGTATGGCAGTTGGAAGTTATTTCAGTCATTTG
    TTTACAGCTCAAACAGGGATTAATGTTCCAATTTACGTTGGCTCATTATTTGTAGCTGTT
    ATTGTCCGAAATATCTCTGAAAGTTTTAATTTTAATATTGTAGATTTAAAAATTACTAAT
    CAAATTGGCGATGTCGCATTAGGTATTTTCTTATCTCTTGCGCTAATGAGCATTCAATTA
    ATCGAAATTTATAAACTTGCTATACCTCTTATTATTATCGTTTTAGTTCAAGTTGTCGTT
    ATGATTTTATTTGCTGTTTTAATTTTATTTAGAGGTTTAGGAAAAGATTATGATGCTGCA
    GTAATGGTAGGTGGTTTTATCGGTCATGGGCTTGGTGCAACGCCAAATGCCATGGCAAAT
    TTAGATGTTATTACTAAAAAATATGGAAACTCACCTAAAGCATATTTAGTTGTACCTATT
    GTTGGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGG
    TTTAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1209

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2246 [new locus tag: NWMN_RS12935 ]
  • symbol: NWMN_2246
  • description: sodium/glutamate symporter
  • length: 402
  • theoretical pI: 8.86246
  • theoretical MW: 43263.7
  • GRAVY: 1.01219

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminessodium/glutamate symporter (TIGR00210; HMM-score: 358)
  • TheSEED:  
    Sodium/glutamate symport protein 
  • PFAM:
    CPA_AT (CL0064) Glt_symporter; Sodium/glutamate symporter (PF03616; HMM-score: 505.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.132
    • Ymax_pos: 13
    • Cmax: 0.153
    • Cmax_pos: 57
    • Smax: 0.213
    • Smax_pos: 8
    • Smean: 0.176
    • D: 0.149
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLIFAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFCAIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTAALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATEVFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDLKITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLGKDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVIVIMGFIQWFS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]