From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2278 [new locus tag: NWMN_RS13115 ]
  • pan locus tag?: SAUPAN005896000
  • symbol: NWMN_2278
  • pan gene symbol?: cobI
  • synonym:
  • product: CorA-like Mg2+ transporter protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2278 [new locus tag: NWMN_RS13115 ]
  • symbol: NWMN_2278
  • product: CorA-like Mg2+ transporter protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2501735..2502679
  • length: 945
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGATTACTTCATTCAGACATTCTGAAGATATAGATAAACATATTATAAAAACACCATTA
    GATCATACAGCGTCATGGATTAATGTAGTAGAACCAGACCGAGAAGAAATTGAAAATCTT
    ATGGAACAATATAATATACCTGAAGACTTTATACGTGACCCTTTGGACTCAGAAGAAAGT
    TCCCGTATTGAATATGACGAAGATACTGGTTACTCATTAATCATTATTGATTTACCTATC
    GTCAATTCAACTAATCGTAGCGTTCTATCTTTTGTAACGATTCCATTAGGCATTATTATT
    GGCAACGGTATTATCGTAACAGTTTGTGACGCTGAAAATGAATTTTTAGAAAATTTACCT
    AAGCGTGATATTAATTTAAAATTTCACAGCAGATTTGCATTAGAAATTTTAACTACTATT
    GCCGACCATTATAATCGTAACTTACGATTACTTAATAAAAGTAGAATTCGTATTGAGAAA
    GAACTAAAAAATAACATTACTAACAAACAACTTTTCAAATTAATGGAAGTTGAAAAAAGT
    TTAGTATACTTTTTAGCTGCCTTAAAAGGTAACGATACAATTATTAAAAAGTTATTCCGT
    TTACCTGCAATTAAACGTTTTGAAGAAGATGAAGAATTACTAGAAGATTTAATCATTGAA
    AATAACCAAGCCATCGAAACAACAGAATTACATCAACGTATCTTAGAAAGTATCACAACA
    TCATACGCTTCCTTATTATCTAATGATATGAATACGATTATGAAGACATTAACACTTTTC
    ACGGTACTATTAACGTTACCAACACTCGTATTTAGTTTCTTCGGTATGAATGTGCCGTTG
    CCAATTGATGACCATAGTTATATATCTTGGATTATTGTGGTAGGTATTTCATTAATTCTT
    GTCGTAATTGTTAGTATTTTCTTATGGCGTAAACAGAAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    945

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2278 [new locus tag: NWMN_RS13115 ]
  • symbol: NWMN_2278
  • description: CorA-like Mg2+ transporter protein
  • length: 314
  • theoretical pI: 4.71169
  • theoretical MW: 36360.8
  • GRAVY: 0.038535

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmagnesium and cobalt transport protein CorA (TIGR00383; HMM-score: 100.9)
  • TheSEED:  
    Magnesium and cobalt transport protein CorA 
  • PFAM:
    no clan definedCorA; CorA-like Mg2+ transporter protein (PF01544; HMM-score: 198)
    DUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 20.2)
    SIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 10.5)
    CPA_AT (CL0064) Mem_trans; Membrane transport protein (PF03547; HMM-score: 10.3)
    Tetraspannin (CL0347) CD20; CD20-like family (PF04103; HMM-score: 9.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.78
    • Cytoplasmic Membrane Score: 8.16
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.099
    • Ymax_pos: 69
    • Cmax: 0.106
    • Cmax_pos: 38
    • Smax: 0.112
    • Smax_pos: 39
    • Smean: 0.083
    • D: 0.093
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQYNIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGNGIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEKELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLIIENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGMNVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]