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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2342 [new locus tag: NWMN_RS13495 ]
  • pan locus tag?: SAUPAN005995000
  • symbol: apbA
  • pan gene symbol?:
  • synonym:
  • product: 2-dehydropantoate 2-reductase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2342 [new locus tag: NWMN_RS13495 ]
  • symbol: apbA
  • product: 2-dehydropantoate 2-reductase
  • replicon: chromosome
  • strand: -
  • coordinates: 2575698..2576633
  • length: 936
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAATTGCAATTGCTGGATCGGGTGCATTAGGTAGTGGCTTTGGTGCCAAACTATTT
    CAAGCAGGATATGATGTCACACTTATTGACGGATATACATCTCATGTTGAAGCGGTTAAG
    CAACATGGATTAAATATAACGATTAATGGAGAGGCATTCGAGTTAAACATTCCGATGTAT
    CATTTTAATGATCAACCGGACGAAAGCATTTACGATGTTGTCTTTCTATTTCCAAAGTCT
    ATGCAATTAAAAGAAGTGATGGAAGATATGAAGCCACATATTGATAATGAAACGATCGTC
    GTATGTACGATGAATGGTCTGAAGCATGAAGAAGTCATTGCGCAGTATGTTGCTCAATCA
    CAAATTGTCAGAGGTGTTACGACTTGGACGGCAGGTCTTGAAAGCCCTGGACACAGTCAT
    TTACTTGGTAGTGGACCAGTTGAAATAGGTGAACTAGTGGATGAAGGTAAAGAAAATGTT
    ATAAAAGTTGCTGATTTACTTAACGAAGCGGAATTGAATGGTGTCATTAGTAAAGATTTA
    TACCAATCGATTTGGAAAAAGATTTGTGTTAATGGTACGGCAAATGCATTAAGCACAGTG
    TTGGAGTGTAATATGGCATCGCTGAATGAAAGTAGTTATGCGAAGTGTTTGATTTATAAA
    TTAACGCAAGAAATAGTGCATGTAGCGACGATTGATAATGTTCATTTAAATGTTGATGAA
    GTATTTGAATATTTAGTTGATTTAAATGAAAAAGTTGGTGCGCATTATCCATCCATGTAT
    CAAGATTTAATTGTTAATAATAGAAAAACTGAAATTGATTATATTAATGGCGCAGTTGCA
    ACATTAGGTAAACAACGTCATATTGAAGCGCCAGTCAATCGCTTTATTACTGATTTAATT
    CATACTAAAGAAAGTCAGCGACACGCACAGGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2342 [new locus tag: NWMN_RS13495 ]
  • symbol: ApbA
  • description: 2-dehydropantoate 2-reductase
  • length: 311
  • theoretical pI: 4.7892
  • theoretical MW: 34443.9
  • GRAVY: -0.117363

Function[edit source | edit]

  • reaction:
    EC 1.1.1.169?  ExPASy
    2-dehydropantoate 2-reductase(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPantothenate and coenzyme A2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 306)
    Cellular processesCellular processesToxin production and resistancetellurite resistance protein TehB (TIGR00477; HMM-score: 16.8)
    MetabolismEnergy metabolismElectron transportNADPH-dependent F420 reductase (TIGR01915; HMM-score: 15.3)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 14.6)
    selenium-dependent molybdenum hydroxylase system protein, YqeB family (TIGR03309; HMM-score: 11.6)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsCoenzyme ACoenzyme A Biosynthesis 2-dehydropantoate 2-reductase (EC 1.1.1.169) 
  • PFAM:
    NADP_Rossmann (CL0063) ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 130.4)
    6PGD_C (CL0106) ApbA_C; Ketopantoate reductase PanE/ApbA C terminal (PF08546; HMM-score: 113.4)
    NADP_Rossmann (CL0063) NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 24.8)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 22.8)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 20.6)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 20)
    no clan definedDUF4172; Domain of unknown function (DUF4172) (PF13776; HMM-score: 17.7)
    NADP_Rossmann (CL0063) UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 16.7)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 15.7)
    TehB; Tellurite resistance protein TehB (PF03848; HMM-score: 13.5)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 11.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.83
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.177
    • Ymax_pos: 23
    • Cmax: 0.144
    • Cmax_pos: 23
    • Smax: 0.366
    • Smax_pos: 19
    • Smean: 0.21
    • D: 0.19
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGLNITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVCTMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENVIKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLIYKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYINGAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]