NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2351 [new locus tag: NWMN_RS13550 ]
• pan locus tag^?: SAUPAN006008000
• symbol: NWMN_2351
• pan gene symbol^?: —
• synonym:
• product: general substrate transporter involved in chloramphenicol resistance

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2351 [new locus tag: NWMN_RS13550 ]
• symbol: NWMN_2351
• product: general substrate transporter involved in chloramphenicol resistance
• replicon: chromosome
• strand: -
• coordinates: 2587172..2588359
• length: 1188
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331439 NCBI
• RefSeq: YP_001333385 NCBI
• MicrobesOnline: 3707953 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1141
  
  TTGTCAATCATGCGACTATTTACATTCATTTTAAGTATTTTTATCGTAGGAATGGTTGAA
  ATGATGGTTGCAGGAATTATGAACTTGATGAGTCAGGACTTACATGTATCAGAAGCTGTC
  GTTGGTCAATTAGTGACAATGTACGCTTTAACATTTGCGATATGTGGACCTATTCTGGTT
  AAATTAACGAACCGTTTTTCATCAAGGCCTGTATTATTATGGACATTACTTATATTTATC
  ATTGGTAATGGCATTATTGCTGTAGCGCCAAATTTTTCAATATTAGTAGTTGGTAGAATT
  ATCTCATCTGCAGCAGCAGCACTAATTATCGTAAAAGTATTAGCTATTACAGCGATGTTA
  TCAGCACCTAAAAATCGTGGTAAAATGATTGGACTTGTCTATACAGGGTTTAGTGGTGCT
  AATGTTTTTGGTGTACCAATTGGAACGGTTATCGGCGATTTAGTAGGTTGGCGCTATACA
  TTTCTATTCTTAATTATTGTGAGTATTATTGTTGGCTTCTTGATGATGATCTATTTACCG
  AAGGATCAGGAAATACAACGAGGCCCTGTGAATCATGAGACACCATCTCATGAAAATCAT
  GTTACTTCGAAAATATTAAGACCTGCTGAAGTAGCAAAATATTTAATTATTACGTTTTTA
  GTATTGATTGCAAACTCAGTGACATTCGTCTTTATAAATCCACTTATTTTATCTAATGGT
  CATGATATGTCATTCGTTTCATTAGCACTTCTAGTAAATGGAATCGCTGGCGTTATTGGA
  ACATCATTAGGTGGTATATTCTCCGATAAAATTACAAGTAAGCGTTGGTTAATGATTTCT
  GTTTCTATTTTTATCGTCATGATGTTACTTATGAATTTAATCTTACCTGGTTCAGGTCTA
  TTGTTAGCAGGACTATTTATTTGGAATATCATGCAATGGAGTACTAATCCAGCAGTGCAA
  AGCGGTGTGATTCAACATGTTGAAGGCGACACAAGCCAAGTAATGAGTTGGAACATGTCT
  AGTTTAAACGCTGGTATTGGTGTTGGAGGCATTATTGGAGGCTTGGTCATGACACATGTT
  TCTGTTCAAGCTATCACATATACGAGTGCCATCATTGGCGCATTAGGATTAATCGTTGTT
  TTCACATTGAAAAATAATCATTATGCTAAAACATTTAAATCATCATAA
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  1140
  1188
  

⊟Protein

⊟General

• locus tag: NWMN_2351 [new locus tag: NWMN_RS13550 ]
• symbol: NWMN_2351
• description: general substrate transporter involved in chloramphenicol resistance
• length: 395
• theoretical pI: 10.1636
• theoretical MW: 42574.9
• GRAVY: 0.966076

⊟Function

• reaction:
• TIGRFAM:
  multidrug resistance protein (TIGR00880; HMM-score: 96.1)
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 71.8)
  Cellular processesCellular processesToxin production and resistancedrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 65.1)
  MetabolismTransport and binding proteinsOtherdrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 65.1)
  MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-galactonate transporter (TIGR00893; HMM-score: 46.9)
  MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 41.1)
  MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsoxalate/formate antiporter family transporter (TIGR00890; HMM-score: 36)
  MetabolismTransport and binding proteinsOthercyanate transporter (TIGR00896; HMM-score: 25.2)
  MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsphosphoglycerate transporter family protein (TIGR00881; HMM-score: 24.2)
  MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 22.3)
  MetabolismTransport and binding proteinsCations and iron carrying compoundscation transport protein (TIGR00898; HMM-score: 9.2)
• TheSEED:  
  Chloramphenicol resistance protein 
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 199)
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 27.1)
  MFS_1_like; MFS_1 like family (PF12832; HMM-score: 12.7)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular Possibility: 0.17
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.197
  • Ymax_pos: 11
  • Cmax: 0.177
  • Cmax_pos: 40
  • Smax: 0.451
  • Smax_pos: 2
  • Smean: 0.39
  • D: 0.273
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 151222563 NCBI
• RefSeq: YP_001333385 NCBI
• UniProt: A0A0H3KIP7 UniProt

⊟Protein sequence

• MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIISSAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2351 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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