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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2487 [new locus tag: NWMN_RS14275 ]
  • pan locus tag?: SAUPAN006268000
  • symbol: NWMN_2487
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2487 [new locus tag: NWMN_RS14275 ]
  • symbol: NWMN_2487
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2732881..2733747
  • length: 867
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAATGAACAATGGTTAGAGCATTTACCTTTAAAAGATATTAAAGAGATTTCACCAGTG
    AGTGGTGGTGATGTAAACGAAGCATATCGAGTCGAAACAGATACGGATACATTTTTCTTA
    CTTGTCCAACGTGGACGTAAAGAATCATTTTATGCTGCAGAAATTGCAGGTTTAAATGAA
    TTTGAACGTGCAGGTATCACGGCACCTAGAGTAATTGCAAGTGGCGAGGTTAACGGTGAT
    GCGTATTTAGTGATGACGTATTTAGAAGAAGGGGCTTCAGGGAGTCAACGCCAATTAGGG
    CAACTCGTAGCTCAATTACACAGTCAGCAACAAGAAGAAGGCAAATTTGGCTTCTCATTA
    CCTTATGAAGGTGGCGATATTTCTTTTGATAATCATTGGCAAGACGATTGGTGTACCATT
    TTTGTCGACAAACGCTTGGACCATTTGAAAGATGAGTTGCTAAATAGAGGGCTATGGGAT
    GCCAACGATATCAAAGTATATGACAAAGTGCGACGTCAAATTGTGGCGGAATTAGAAAAG
    CATCAAAGTAAACCGTCTTTATTACATGGTGACCTATGGGGTGGTAATTATATGTTCTTA
    CAAGATGGTCGTCCGGCGTTATTTGATCCAGCGCCATTATATGGTGACAGAGAATTCGAT
    ATCGGTATTACAACGGTATTTGGTGGTTTTACGAGCGAATTTTATGATGCGTATAATAAA
    CATTATCCACTCGCAAAAGGTGCATCCTATAGACTTGAATTTTATCGTTTATATTTATTG
    ATGGTCCATTTATTGAAATTTGGTGAGATGTACCGTGATAGTGTTGCGCATTCTATGGAT
    AAGATTTTACAAGATACAACAAGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    867

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2487 [new locus tag: NWMN_RS14275 ]
  • symbol: NWMN_2487
  • description: hypothetical protein
  • length: 288
  • theoretical pI: 4.67614
  • theoretical MW: 32987.7
  • GRAVY: -0.456944

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    fibrobacter succinogenes paralogous family TIGR02172 (TIGR02172; HMM-score: 12.1)
    S-methyl-5-thioribose kinase (TIGR01767; EC 2.7.1.100; HMM-score: 11.6)
  • TheSEED:  
    Fructosamine kinase family protein, At3g61080 homolog 
  • PFAM:
    PKinase (CL0016) Fructosamin_kin; Fructosamine kinase (PF03881; HMM-score: 254.3)
    APH; Phosphotransferase enzyme family (PF01636; HMM-score: 68.1)
    APH_6_hur; Aminoglycoside/hydroxyurea antibiotic resistance kinase (PF04655; HMM-score: 16.7)
    EcKinase; Ecdysteroid kinase (PF02958; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.1
    • Ymax_pos: 12
    • Cmax: 0.115
    • Cmax_pos: 24
    • Smax: 0.139
    • Smax_pos: 4
    • Smean: 0.102
    • D: 0.101
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQRGRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQLVAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWDANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDREFDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVAHSMDKILQDTTS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SigB [1] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]