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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03020 [old locus tag: NWMN_0521 ]
  • pan locus tag?: SAUPAN002332000
  • symbol: NWMN_RS03020
  • pan gene symbol?:
  • synonym:
  • product: HAD family phosphatase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS03020 [old locus tag: NWMN_0521 ]
  • symbol: NWMN_RS03020
  • product: HAD family phosphatase
  • replicon: chromosome
  • strand: +
  • coordinates: 599782..600651
  • length: 870
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (599782..600651) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    481
    541
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    841
    ATGATAAAACTAATAGCCACTGATATGGATGGCACGCTACTTAATGCAGCACATGAAATT
    TCTCAACCTAATATTGATGCGATTAAATACGCTCAAGAACAAGGGATAACGGTTGTTATC
    GCGACAGGTCGAGCATTTTATGAAGCACAAGCACCAGTTGCTGACACAGATTTAACAGTA
    CCATATATTTGTTTGAATGGTGCTGAAGTACGTGATGAAACTTTCAATGTAATGAGCACT
    TCACACCTTAATAAATCGTTAGTACACAAAATTACAAATGTTTTAAAAGATGCAGGTATT
    TATTATCAAGTATACACGAGTCGTGCGATTTATACTGAAGATCCACAAAGAGATTTAGAC
    ATTTACATAGATATTGCTGAGCGTGCAGGTCAACATGCAAACGTTGAGCGTATTAAAAAT
    GGTATTCAAAGACGCATAGATAATGGTACGTTGAAAGTTGTTGATAATTATGATGCTATT
    GAAAACATACCTGGTGAATTAATTATGAAAATATTAGCATTTGATGGAAATTTAGAAAAA
    ATTGACAAAGCTAGTAAAATTTTAGCTGAATCTCCGAATTTAGCTATATCATCATCTTCG
    AGAGGAAATATAGAAATAACGCATTCAGATGCACAAAAAGGTATTGCGCTAGAAACAATT
    GCCGAAAGATTAGGGATTGAAATGAAAGAAGTCATGTCAATAGGTGACAATTTAAATGAC
    TTATCAATGTTAGAGAAAGTTGGCTATCCAGTTGCGATGGAAAATGGTGCAGAAGAAGTT
    AAAAAAATAGCGAAATATGTCACAGATACGAATGAAAATAGTGGTGTTGGAAAAGCTATT
    ATGAAATTATTACGTGAACAACAAGTTTAA
    60
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    870

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS03020 [old locus tag: NWMN_0521 ]
  • symbol: NWMN_RS03020
  • description: HAD family phosphatase
  • length: 289
  • theoretical pI: 4.65575
  • theoretical MW: 31841
  • GRAVY: -0.225606

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityCof-like hydrolase (TIGR00099; HMM-score: 208.8)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 113.1)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIB (TIGR01484; HMM-score: 91.4)
    sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 85.2)
    mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 59)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharides3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 54.2)
    Unknown functionGeneralmannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 48.2)
    mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 45.3)
    MetabolismEnergy metabolismAnaerobicphenylphosphate carboxylase, delta subunit (TIGR02726; HMM-score: 37.1)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIIA (TIGR01662; HMM-score: 34.2)
    phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 31.6)
    MetabolismAmino acid biosynthesisSerine familyphosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 31.2)
    sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 24)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IB (TIGR01490; HMM-score: 21.2)
    sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 19.3)
    Unknown functionEnzymes of unknown specificityHAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 18.3)
    MetabolismEnergy metabolismSugarsphosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 16.8)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 15.2)
  • TheSEED:
  • PFAM:
    HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 244.3)
    S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 48.1)
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 33.5)
    HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 16.9)
    Acid_phosphat_B; HAD superfamily, subfamily IIIB (Acid phosphatase) (PF03767; HMM-score: 13.8)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 13.6)
    no clan definedHrpF; HrpF protein (PF06266; HMM-score: 13.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.125
    • Ymax_pos: 11
    • Cmax: 0.14
    • Cmax_pos: 55
    • Smax: 0.183
    • Smax_pos: 16
    • Smean: 0.155
    • D: 0.137
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446516021 NCBI
  • RefSeq: WP_000593367 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGRAFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYYQVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAIENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALETIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGVGKAIMKLLREQQV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS03020 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]