From AureoWiki
Jump to: navigation, search

NCBI: 02-MAR-2017

⊟Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03100 [old locus tag: NWMN_0539 ]
  • pan locus tag?: SAUPAN002353000
  • symbol: NWMN_RS03100
  • pan gene symbol?: vraB
  • synonym:
  • product: acetyl-CoA acetyltransferase

⊟Genome View[edit source | edit]

⊟Gene[edit source | edit]

⊟General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS03100 [old locus tag: NWMN_0539 ]
  • symbol: NWMN_RS03100
  • product: acetyl-CoA acetyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 625015..626154
  • length: 1140
  • essential: unknown

⊟Accession numbers[edit source | edit]

  • Location: NC_009641 (625015..626154) NCBI
  • MicrobesOnline:

⊟Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAATCAAGCAGTCATAGTTGCAGCTAAACGAACTGCATTTGGGAAATATGGTGGCACT
    TTAAAACATTTAGAGCCAGAACAATTGCTTAAACCTTTATTCCAACATTTTAAAGAGAAG
    TATCCAGAGGTAATATCTAAAATAGATGATGTAGTTTTAGGTAATGTTGTTGGGAATGGT
    GGCAATATTGCAAGAAAAGCATTGCTTGAAGCGGGGCTTAAAGATTCAATACCTGGCGTC
    ACAATCGATCGGCAATGTGGGTCTGGACTTGAAAGTGTTCAATATGCATGTCGCATGATC
    CAAGCCGGAGCTGGCAAGGTATATATTGCAGGTGGTGTTGAAAGTACAAGTCGAGCACCT
    TGGAAAATCAAACGACCGCATTCTGTGTACGAAACAGCATTACCTGAGTTTTATGAGCGT
    GCATCATTTGCACCTGAAATGAGCGACCCATCAATGATTCAAGGTGCTGAAAATGTGGCC
    AAGATGTATGATGTTTCAAGAGAATTACAAGATGAATTTGCTTATCGAAGTCATCAATTG
    ACAGCGGAAAATGTAAAGAATGGAAATATTTCTCAGGAAATATTACCTATAACCGTTAAA
    GGAGAAATATTCAACACTGATGAAAGTCTAAAATCACATATTCCGAAAGATAACTTTGGC
    CGATTTAAGCCCGTGATCAAAGGTGGGACCGTTACCGCTGCGAATAGTTGTATGAAAAAT
    GATGGTGCAGTTTTATTGCTTATTATGGAAAAAGATATGGCATACGAATTAGGTTTCGAG
    CATGGTTTATTATTTAAAGATGGTGTTACGGTAGGTGTTGATTCTAATTTTCCTGGCATT
    GGTCCAGTACCAGCCATTTCCAACTTACTAAAAAGAAATCAATTAACGATAGAAAATATT
    GAAGTCATTGAAATTAACGAAGCGTTCAGTGCACAGGTAGTTGCCTGCCAACAAGCTTTA
    AATATTTCAAATACGCAATTAAATATATGGGGTGGTGCATTAGCATCAGGTCATCCATAC
    GGTGCAAGCGGTGCCCAATTAGTGACTCGATTATTTTATATGTTTGACAAAGAGACTATG
    ATTGCATCTATGGGGATAGGGGGAGGTCTAGGAAATGCAGCATTATTTACTCGATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140

⊟Protein[edit source | edit]

⊟General[edit source | edit]

  • locus tag: NWMN_RS03100 [old locus tag: NWMN_0539 ]
  • symbol: NWMN_RS03100
  • description: acetyl-CoA acetyltransferase
  • length: 379
  • theoretical pI: 6.6333
  • theoretical MW: 41093.8
  • GRAVY: -0.0957784

⊟Function[edit source | edit]

  • TIGRFAM:
    MetabolismFatty acid and phospholipid metabolismOtheracetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 383.7)
    and 4 more
    3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 261.3)
    MetabolismFatty acid and phospholipid metabolismDegradationacetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 211.7)
    MetabolismFatty acid and phospholipid metabolismDegradationacetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 126.2)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisbeta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 15.9)
  • TheSEED:
  • PFAM:
    Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 191)
    and 2 more
    Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 104.3)
    ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 19.4)

⊟Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

⊟Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.132
    • Ymax_pos: 12
    • Cmax: 0.108
    • Cmax_pos: 16
    • Smax: 0.209
    • Smax_pos: 6
    • Smean: 0.175
    • D: 0.149
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit source | edit]

  • GI: 446993420 NCBI
  • RefSeq: WP_001070676 NCBI
  • UniProt:

⊟Protein sequence[edit source | edit]

  • MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQAGAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVAKMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF

⊟Experimental data[edit source | edit]

  • experimentally validated: no data available

⊟Expression & Regulation[edit source | edit]

⊟Operon[edit source | edit]

  • NWMN_RS03100 no polycistronic organisation predicted

⊟Regulation[edit source | edit]

  • sigma factor:
  • regulator:

⊟Transcription pattern[edit source | edit]

⊟Protein synthesis (provided by Aureolib)[edit source | edit]

⊟Protein stability[edit source | edit]

  • half-life: no data available

⊟Biological Material[edit source | edit]

⊟Mutants[edit source | edit]

⊟Expression vector[edit source | edit]

⊟lacZ fusion[edit source | edit]

⊟GFP fusion[edit source | edit]

⊟two-hybrid system[edit source | edit]

⊟FLAG-tag construct[edit source | edit]

⊟Antibody[edit source | edit]

⊟Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

⊟Literature[edit source | edit]

⊟References[edit source | edit]

⊟Relevant publications[edit source | edit]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_RS03100&oldid=80450"