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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03140 [old locus tag: NWMN_0547 ]
  • pan locus tag?: SAUPAN002362000
  • symbol: NWMN_RS03140
  • pan gene symbol?:
  • synonym:
  • product: amino acid permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS03140 [old locus tag: NWMN_0547 ]
  • symbol: NWMN_RS03140
  • product: amino acid permease
  • replicon: chromosome
  • strand: +
  • coordinates: 630314..631798
  • length: 1485
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (630314..631798) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    361
    421
    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAAAAAAAGAATAAGCAAATAGATAGAGGCGATTTAAAACAAAACCTATCTGAAAAG
    TTTGTATGGGCGATTGCATATGGTTCATGTATCGGATGGGGCGCATTCATCTTACCAGGA
    GACTGGATTAAGCAGTCAGGTCCGATTGCAGCATCAATTGGTATAGTTATTGGTGCATTA
    TTAATGATATTAATTGCGGTTAGTTATGGCGCATTAGTAGAGAGATTTCCAGTATCAGGG
    GGCGCGTTTGCCTTTAGTTTCTTAAGTTTCGGCAGATATGTGAGTTTCTTCTCATCATGG
    TTTTTAACTTTTGGTTATGTCTGTGTCGTTGCTTTAAATGCGACCGCATTCAGTTTACTA
    GTTAAATTCTTATTGCCAGATGTCTTAAATAATGGGAAACTATACACCATTGCGGGCTGG
    GACGTTTATATTACGGAAATCATTATTGCGACCGTATTACTACTTGTATTCATGCTAGTA
    ACGATTCGTGGCGCAAGTGTATCTGGATCATTACAATATTATTTCTGTGTGGCGATGGTA
    ATCGTCGTATTATTGATGTTCTTTGGTTCATTCTTTGGTAATAATTTTGCACTTGAAAAT
    TTACAACCGTTAGCTGAACCTAGCAAAGGATGGTTAGTGTCTATTGTGGTTATTGTATCC
    GTGGCACCATGGGCATATGTTGGATTTGATAATATTCCACAAACAGCAGAAGAGTTTAAC
    TTTGCACCAAACAAGACATTTAAGCTTATCGTGTACAGTTTATTAGCAGCATCATTAACT
    TATGTTGTCATGATTTTATACACTGGTTGGTTATCAACAAGTCATCAAAGTTTAAATGGG
    CAGTTGTGGTTAACAGGTGCTGTTACACAAACAGCATTTGGTTATATTGGATTAGGTGTA
    TTAGCAATTGCAATTATGATGGGTATATTTACTGGTTTAAATGGATTCTTGATGAGTTCA
    AGTCGCTTGTTATTTTCTATGGGACGTTCAGGTATTATGCCAACAATGTTTAGTAAATTA
    CATAGTAAATACAAAACACCATATGTCGCAATCATATTCCTAGTAGGAGTGTCGTTAATT
    GCACCTTGGCTAGGAAGAACTGCATTGACTTGGATTGTAGATATGTCATCTACTGGTGTA
    TCCATTGCCTACTTTATTACATGTTTGTCTGCAGCGAAATTATTCAGTTATAACAAACAA
    AGTAATACGTATGCACCGGTTTACAAAACGTTTGCTATTATCGGCTCATTTGTATCATTC
    ATTTTCTTAGCGTTGTTATTAGTGCCAGGTTCTCCTGCAGCACTGACTGCACCGTCTTAT
    ATTGCATTACTTGGATGGTTAATCATCGGTTTAATATTCTTTGTGATTCGATATCCTAAA
    TTGAAAAATATGGATAATGATGAATTAAGTCGCTTGATTTTAAATAGAAGTGAAAATGAA
    GTTGATGATATGATTGAAGAACCTGAAAAAGAAAAAACTAAATAA
    60
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    1260
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    1440
    1485

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS03140 [old locus tag: NWMN_0547 ]
  • symbol: NWMN_RS03140
  • description: amino acid permease
  • length: 494
  • theoretical pI: 9.05617
  • theoretical MW: 54336.8
  • GRAVY: 0.731579

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid transporter (TIGR00909; HMM-score: 129)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminescationic amino acid transport permease (TIGR00906; HMM-score: 75.3)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminestransporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 65.8)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesL-type amino acid transporter (TIGR00911; HMM-score: 56.8)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid permease (yeast) (TIGR00913; HMM-score: 48.7)
    permease, urea carboxylase system (TIGR03428; HMM-score: 47.2)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesethanolamine permease (TIGR00908; HMM-score: 35.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesarginine-ornithine antiporter (TIGR03810; HMM-score: 29.8)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesGABA permease (TIGR01773; HMM-score: 23.2)
  • TheSEED:
  • PFAM:
    APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 140.4)
    AA_permease; Amino acid permease (PF00324; HMM-score: 73.7)
    no clan definedDUF2615; Protein of unknown function (DUF2615) (PF11027; HMM-score: 8.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.116
    • Ymax_pos: 45
    • Cmax: 0.132
    • Cmax_pos: 52
    • Smax: 0.137
    • Smax_pos: 2
    • Smean: 0.091
    • D: 0.106
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

  • GI: 446334659 NCBI
  • RefSeq: WP_000412514 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIKQSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFLTFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLVTIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVIVSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQSLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMPTMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAAKLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIGLIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS03140 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]