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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03565 [old locus tag: NWMN_0627 ]
  • pan locus tag?: SAUPAN002537000
  • symbol: NWMN_RS03565
  • pan gene symbol?: graX
  • synonym:
  • product: NAD(P)-dependent oxidoreductase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS03565 [old locus tag: NWMN_0627 ]
  • symbol: NWMN_RS03565
  • product: NAD(P)-dependent oxidoreductase
  • replicon: chromosome
  • strand: +
  • coordinates: 708437..709360
  • length: 924
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (708437..709360) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAACCTAAAGTTTTATTAGCAGGTGGAACAGGATATATTGGTAAGTATTTAAGTGAA
    GTGATTGAAAATGATGCTGAACTTTTTGCTATATCAAAATATCCAGACAATAAAAAAACA
    GATGATGTTGAAATGACTTGGATTCAGTGTGATATATTTCATTACGAACAGGTTGTTGCA
    GCAATGAATCAAATAGATATTGCTGTATTCTTTATCGACCCAACAAAGAATTCTGCCAAA
    ATAACACAATCATCAGCAAGAGATTTAACATTAATCGCAGCAGATAATTTTGGTCGAGCA
    GCGGCTATCAATCAAGTAAAAAAAGTAATCTACATACCTGGGAGTCGTTATGATAATGAA
    ACAATTGAACGCCTAGGTGCATATGGTACACCTGTAGAAACAACAAATTTAGTTTTTAAA
    CGTTCTTTAGTTAATGTAGAATTACAAGTTTCAAAGTATGATGATGTTAGATCAACGATG
    AAGGTAGTTTTACCAAAGGGATGGACATTAAAGAACGTTGTAAACCATTTTATTGCATGG
    ATGGGTTACACTAAAGGAACTTTTGTGAAAACAGAAAAATCACATGATCAATTTAAGATA
    TATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGA
    ATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGG
    AAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTAT
    ATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATG
    ATTCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGA
    GAAAATATGAAATATACTAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    924

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS03565 [old locus tag: NWMN_0627 ]
  • symbol: NWMN_RS03565
  • description: NAD(P)-dependent oxidoreductase
  • length: 307
  • theoretical pI: 9.62557
  • theoretical MW: 35234.8
  • GRAVY: -0.14886

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Hypothetical proteinsConservedTIGR01777 family protein (TIGR01777; HMM-score: 13)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 23.5)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 22.8)
    3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 15.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.114
    • Ymax_pos: 31
    • Cmax: 0.116
    • Cmax_pos: 62
    • Smax: 0.18
    • Smax_pos: 23
    • Smean: 0.11
    • D: 0.112
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446725633 NCBI
  • RefSeq: WP_000802946 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVEMTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAAINQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTMKVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETADGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPMQKMMIHGFEVDCRIKDFQSRLKSGENMKYTK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS03565 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]