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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS04200 [old locus tag: NWMN_0742 ]
  • pan locus tag?: SAUPAN002707000
  • symbol: NWMN_RS04200
  • pan gene symbol?: pgk
  • synonym:
  • product: phosphoglycerate kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS04200 [old locus tag: NWMN_0742 ]
  • symbol: NWMN_RS04200
  • product: phosphoglycerate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 835105..836295
  • length: 1191
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (835105..836295) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCTAAAAAAATTGTTTCTGATTTAGATCTTAAAGGTAAAACAGTCCTAGTACGTGCT
    GATTTTAACGTACCTTTAAAAGACGGTGAAATTACTAATGACAACCGTATCGTTCAAGCT
    TTACCTACAATTCAATACATCATCGAACAAGGTGGTAAAATCGTACTATTTTCACATTTA
    GGTAAAGTGAAAGAAGAAAGTGATAAAGCAAAATTAACTTTACGTCCAGTTGCTGAAGAC
    TTATCTAAGAAATTAGATAAAGAAGTTGTTTTCGTACCAGAAACACGCGGCGAAAAACTT
    GAAGCTGCTATTAAAGACCTTAAAGAAGGCGACGTATTATTAGTTGAAAATACACGTTAT
    GAAGATTTAGACGGTAAAAAAGAATCTAAAAATGATCCAGAATTAGGTAAATACTGGGCA
    TCTTTAGGTGATGTGTTTGTAAATGATGCTTTTGGTACTGCGCATCGTGAGCATGCATCT
    AATGTTGGTATTTCTACACATTTAGAAACTGCAGCTGGATTCTTAATGGATAAAGAAATT
    AAGTTTATTGGCGGCGTAGTTAACGATCCACATAAACCAGTTGTTGCTATTTTAGGTGGA
    GCAAAAGTATCTGACAAAATTAATGTCATCAAAAACTTAGTTAACATAGCTGATAAAATT
    ATCATCGGCGGAGGTATGGCTTATACTTTCTTAAAAGCGCAAGGTAAAGAAATTGGTATT
    TCATTATTAGAAGAAGATAAAATCGACTTCGCAAAAGATTTATTAGAAAAACATGGTGAT
    AAAATTGTATTACCAGTAGACACTAAAGTTGCTAAAGAATTTTCTAATGATGCCAAAATC
    ACTGTAGTACCATCTGATTCAATTCCAGCAGACCAAGAAGGTATGGATATTGGACCAAAC
    ACTGTAAAATTATTTGCAGATGAATTAGAAGGTGCGCACACTGTTGTATGGAATGGACCT
    ATGGGTGTATTCGAGTTCAGTAACTTTGCACAAGGTACAATTGGTGTATGTAAAGCAATT
    GCAAACCTTAAAGATGCAATTACGATTATCGGTGGCGGTGATTCAGCTGCAGCAGCAATC
    TCTTTAGGTTTTGAAAATGACTTCACTCATATTTCAACTGGTGGCGGCGCGTCATTAGAG
    TACCTAGAAGGTAAAGAATTGCCTGGTATCAAAGCAATCAATAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1191

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS04200 [old locus tag: NWMN_0742 ]
  • symbol: NWMN_RS04200
  • description: phosphoglycerate kinase
  • length: 396
  • theoretical pI: 4.96143
  • theoretical MW: 42601.4
  • GRAVY: -0.126263

Function[edit source | edit]

  • reaction:
    EC 2.7.2.3?  ExPASy
    Phosphoglycerate kinaseATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
  • TIGRFAM:
  • TheSEED:
  • PFAM:
    no clan definedPGK; Phosphoglycerate kinase (PF00162; HMM-score: 515.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_RS05380pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    NWMN_RS05385pyruvate dehydrogenase E1 component subunit beta  [1] (data from MRSA252)
    NWMN_RS05390dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex  [1] (data from MRSA252)
    NWMN_RS05395dihydrolipoyl dehydrogenase  [1] (data from MRSA252)
    NWMN_RS10560aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B  [1] (data from MRSA252)
    NWMN_RS1226030S ribosomal protein S9  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.147
    • Ymax_pos: 11
    • Cmax: 0.119
    • Cmax_pos: 41
    • Smax: 0.293
    • Smax_pos: 18
    • Smean: 0.211
    • D: 0.172
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446997493 NCBI
  • RefSeq: WP_001074749 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTIQYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEAAIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHASNVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIADKIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSNDAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTIGVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKAINNK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS04200 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]