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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS05165 [old locus tag: NWMN_0922 ]
  • pan locus tag?: SAUPAN003260000
  • symbol: NWMN_RS05165
  • pan gene symbol?: atl
  • synonym:
  • product: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS05165 [old locus tag: NWMN_0922 ]
  • symbol: NWMN_RS05165
  • product: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1020866..1024636
  • length: 3771
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1020866..1024636) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    ATGGCGAAAAAATTCAATTACAAACTACCATCAATGGTTGCATTAACGCTTGTAGGTTCA
    GCAGTCACTGCACATCAAGTTCAAGCAGCTGAGACGACACAAGATCAAACTACTAATAAA
    AACGTTTTAGATAATAATAAAGTTAAAGCAACTACTGAACAAGCAAAAGCTGAGGTAAAA
    AATCCAACGCAAAACATTTCTGGCACTCAAGTATATCAAGACCCTGCTATTGTCCAACCA
    AAAACAGCAAATAACAAAACAGGCAATGCTCAAGTAAGTCAAAAAGTTGATACTGCACAA
    GTAAATGGTGACACTCGTGCTAATCAATCAGCGACTACAAATAATACGCAGCCTGTTGCA
    AAGTCAACAAGCACTACAGCACCTAAAACTAACACTAATGTTACAAATGCTGGTTATAGT
    TTAGTTGATGATGAAGATGATAATTCAGAAAATCAAATTAATCCAGAATTAATTAAATCA
    GCTGCTAAACCTGCAGCTCTTGAAACGCAATATAAAACCGCAGCACCTAAAGCTGCAACT
    ACATCAGCACCTAAAGCTAAAACTGAAGCGACACCTAAAGTAACTACTTTTAGCGCTTCA
    GCACAACCAAGATCAGTTGCTGCAACACCAAAAACGAGTTTGCCAAAATATAAACCACAA
    GTAAACTCTTCAATTAACGATTACATTCGTAAAAATAACTTAAAAGCACCTAAAATTGAA
    GAAGATTATACATCTTACTTCCCTAAATACGCATACCGTAACGGCGTAGGTCGTCCTGAA
    GGTATCGTAGTTCATGATACAGCTAATGATCGTTCGACGATAAATGGTGAAATTAGTTAT
    ATGAAAAATAACTATCAAAACGCATTCGTACATGCATTTGTTGATGGGGATCGTATAATC
    GAAACAGCACCAACGGATTACTTATCTTGGGGTGTCGGTGCAGTCGGTAACCCTAGATTC
    ATCAATGTTGAAATCGTACACACACACGACTATGCTTCATTTGCACGTTCAATGAATAAC
    TATGCTGACTATGCAGCTACACAATTACAATATTATGGTTTAAAACCAGACAGTGCTGAG
    TATGATGGAAATGGTACAGTATGGACTCACTACGCTGTAAGTAAATATTTAGGTGGTACT
    GACCATGCCGATCCACATGGATATTTAAGAAGTCATAATTATAGTTATGATCAATTATAT
    GACTTAATTAATGAAAAATATTTAATAAAAATGGGTAAAGTGGCGCCATGGGGTACGCAA
    TCTACAACTACCCCTACTACACCATCAAAACCAACAACACCGTCGAAACCATCAACTGGT
    AAATTAACAGTTGCTGCAAACAATGGTGTCGCACAAATCAAACCAACAAATAGTGGTTTA
    TATACTACTGTATACGACAAAACTGGTAAAGCAACTAATGAAGTTCAAAAAACATTTGCT
    GTATCTAAAACAGCTACATTAGGTAATCAAAAATTCTATCTTGTTCAAGATTACAATTCT
    GGTAATAAATTTGGTTGGGTTAAAGAAGGCGATGTGGTTTACAACACAGCTAAATCACCT
    GTAAATGTAAATCAATCATATTCAATCAAACCTGGTACGAAACTTTATACAGTACCTTGG
    GGTACATCTAAACAAGTTGCTGGTAGTGTGTCTGGCTCTGGAAACCAAACATTTAAGGCT
    TCAAAGCAACAACAAATTGATAAATCAATTTATTTATATGGCTCTGTGAATGGTAAATCT
    GGTTGGGTAAGTAAAGCATATTTAGTTGATACTGCTAAACCTACGCCTACACCAACACCT
    AAGCCATCAACACCTACAACAAATAATAAATTAACAGTTTCATCATTAAACGGTGTTGCT
    CAAATTAATGCTAAAAACAATGGCTTATTCACTACAGTTTATGACAAAACTGGTAAGCCA
    ACGAAAGAAGTTCAAAAAACATTTGCTGTAACAAAAGAAGCAAGTTTAGGTGGAAACAAA
    TTCTACTTAGTTAAAGATTACAATAGTCCAACTTTAATTGGTTGGGTTAAACAAGGTGAC
    GTTATTTATAACAATGCAAAATCACCTGTAAATGTAATGCAAACATATACAGTAAAACCA
    GGCACTAAATTATATTCAGTACCTTGGGGCACTTATAAACAAGAAGCTGGTGCAGTTTCT
    GGTACAGGTAACCAAACTTTTAAAGCGACTAAGCAACAACAAATTGATAAATCTATCTAT
    TTATTTGGAACTGTAAATGGTAAATCTGGTTGGGTAAGTAAAGCATATTTAGCTGTACCT
    GCTGCACCTAAAAAAGCAGTAGCACAACCAAAAACAGCTGTAAAAGCTTATACTGTTACT
    AAACCACAAACGACTCAAACAGTTAGCAAGATTGCTCAAGTTAAACCAAACAACACTGGT
    ATTCGTGCTTCTGTTTATGAAAAAACAGCGAAAAACGGTGCGAAATATGCAGACCGTACG
    TTCTATGTAACAAAAGAGCGTGCTCATGGTAATGAAACGTATGTATTATTAAACAATACA
    AGCCATAACATCCCATTAGGTTGGTTCAATGTAAAAGACTTAAATGTTCAAAACTTAGGC
    AAAGAAGTTAAAACGACTCAAAAATATACTGTTAATAAATCAAATAACGGCTTATCAATG
    GTTCCTTGGGGTACTAAAAACCAAGTCATTTTAACAGGCAATAACATTGCTCAAGGTACA
    TTTAATGCAACGAAACAAGTATCTGTAGGCAAAGATGTTTATTTATACGGTACTATTAAT
    AACCGCACTGGTTGGGTAAATGCAAAAGATTTAACTGCACCAACTGCTGTGAAACCAACT
    ACATCAGCTGCCAAAGATTATAACTACACTTATGTAATTAAAAATGGTAATGGTTATTAC
    TATGTAACACCAAATTCTGATACAGCTAAATACTCATTAAAAGCATTTAATGAACAACCA
    TTCGCAGTTGTTAAAGAACAAGTCATTAATGGACAAACTTGGTACTATGGTAAATTATCT
    AACGGTAAATTAGCATGGATTAAATCAACTGATTTAGCTAAAGAATTAATTAAGTATAAT
    CAAACAGGTATGACATTAAACCAAGTTGCTCAAATACAAGCTGGTTTACAATATAAACCA
    CAAGTACAACGTGTACCAGGTAAGTGGACAGGTGCTAACTTTAATGATGTTAAGCATGCA
    ATGGATACGAAGCGTTTAGCTCAAGATCCAGCATTAAAATATCAATTCTTACGCTTAGAC
    CAACCACAAAATATTTCTATTGATAAAATTAATCAATTCTTAAAAGGTAAAGGTGTATTA
    GAAAACCAAGGTGCTGCATTTAACAAAGCTGCTCAAATGTATGGCATTAATGAAGTTTAT
    CTTATCTCACATGCCCTATTAGAAACAGGTAACGGTACTTCTCAATTAGCGAAAGGTGCA
    GATGTAGTGAACAACAAAGTTGTAACTAACTCAAACACGAAATACCATAACGTATTTGGT
    ATTGCTGCATATGATAACGATCCTTTACGTGAAGGTATTAAATATGCTAAACAAGCTGGT
    TGGGACACAGTATCAAAAGCAATCGTTGGTGGTGCTAAATTCATCGGCAACTCATATGTA
    AAAGCTGGTCAAAATACACTTTACAAAATGAGATGGAATCCTGCACATCCAGGAACACAC
    CAATATGCTACAGATGTAGATTGGGCTAACATCAATGCTAAAATCATCAAAGGCTACTAT
    GATAAAATTGGCGAAGTCGGCAAATACTTCGACATCCCACAATATAAATAA
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS05165 [old locus tag: NWMN_0922 ]
  • symbol: NWMN_RS05165
  • description: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase
  • length: 1256
  • theoretical pI: 10.1019
  • theoretical MW: 137391
  • GRAVY: -0.620303

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsV-type ATPase, D subunit (TIGR00309; EC 3.6.3.14; HMM-score: 9.2)
  • TheSEED:
  • PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 54.1)
    SH3 (CL0010) SH3_8; SH3-like domain (PF13457; HMM-score: 46.8)
    no clan definedDGOK; 2-keto-3-deoxy-galactonokinase (PF05035; HMM-score: 25.1)
    DUF780; Caenorhabditis elegans protein of unknown function (DUF780) (PF05611; HMM-score: 13.2)
    SH3 (CL0010) SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 10.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide length 30 aa
    • Ymax: 0.842
    • Ymax_pos: 30
    • Cmax: 0.752
    • Cmax_pos: 30
    • Smax: 0.957
    • Smax_pos: 13
    • Smean: 0.936
    • D: 0.878
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446997252 NCBI
  • RefSeq: WP_001074508 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLDNNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVNGDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKSAAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYKPQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGVAQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINAKNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNNTGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGLQYKPQVQRVPGKWTGANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS05165 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]