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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS05260 [old locus tag: NWMN_0939 ]
  • pan locus tag?: SAUPAN003284000
  • symbol: NWMN_RS05260
  • pan gene symbol?: purM
  • synonym:
  • product: phosphoribosylformylglycinamidine cyclo-ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS05260 [old locus tag: NWMN_0939 ]
  • symbol: NWMN_RS05260
  • product: phosphoribosylformylglycinamidine cyclo-ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1042970..1043998
  • length: 1029
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1042970..1043998) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCTAAAGCATATGAACAATCTGGTGTAAATATTCATGCTGGTTATGAAGCTGTAGAA
    AGAATGTCTAGTCATGTTAAACGTACGATGCGTAAAGAAGTTATCGGTGGTTTAGGTGGA
    TTCGGTGCTACATTTGATTTATCACAATTAAATATGACAGCGCCAGTTTTAGTTTCTGGA
    ACAGACGGAGTAGGTACGAAATTAAAACTAGCTATCGACTATGGTAAACATGATTCGATA
    GGTATCGATGCAGTCGCAATGTGTGTTAATGATATTTTAACGACAGGTGCAGAACCATTA
    TATTTTTTAGATTACATTGCTACAAACAAAGTTGTTCCTGAAGTTATTGAACAAATTGTT
    AAAGGTATTAGTGATGCATGTGTTGAAACGAATACTGCACTTATCGGTGGAGAGACTGCT
    GAAATGGGTGAAATGTATCACGAAGGCGAATATGATGTAGCTGGATTTGCTGTTGGAGCA
    GTTGAAAAGGATGACTATGTAGATGGTTCAGAAGTGAAAGAGGGACAAGTTGTTATAGGG
    CTTGCGTCAAGTGGCATTCATTCAAATGGATACAGTTTAGTGCGCAAATTAATTAATGAA
    TCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAA
    CCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCA
    ATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGA
    TATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAATATTTGATTGGTTACAA
    CAACAAGGCAATATAGACACAAATGAAATGTATAACATTTTTAATATGGGTATTGGCTAT
    ACGGTTATCGTTGATGAAAAAGATGTATCACGCGCTTTGAAGATTTTAGCAGAACAAAAT
    GTGGAAGCCTATCAAATTGGTCATATTGTGAAAAATGAGTCAACTGCAATTGAATTGTTG
    GGGGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1029

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS05260 [old locus tag: NWMN_0939 ]
  • symbol: NWMN_RS05260
  • description: phosphoribosylformylglycinamidine cyclo-ligase
  • length: 342
  • theoretical pI: 4.52335
  • theoretical MW: 37016.8
  • GRAVY: -0.0175439

Function[edit source | edit]

  • reaction:
    EC 6.3.3.1?  ExPASy
    Phosphoribosylformylglycinamidine cyclo-ligaseATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylformylglycinamidine cyclo-ligase (TIGR00878; EC 6.3.3.1; HMM-score: 429.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiamine-phosphate kinase (TIGR01379; EC 2.7.4.16; HMM-score: 41.6)
    hydrogenase expression/formation protein HypE (TIGR02124; HMM-score: 41.3)
    MetabolismEnergy metabolismMethanogenesisputative methanogenesis marker protein 2 (TIGR03267; HMM-score: 32.6)
    selenide, water dikinase (TIGR00476; EC 2.7.9.3; HMM-score: 24.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylformylglycinamidine synthase II (TIGR01736; EC 6.3.5.3; HMM-score: 22.2)
    Unknown functionEnzymes of unknown specificityAIR synthase-related protein, sll0787 family (TIGR04049; HMM-score: 17.6)
    Unknown functionEnzymes of unknown specificityAIR synthase-related protein, MSMEG_0567 C-terminal family (TIGR04050; HMM-score: 17.3)
    bacteriocin-type signal sequence (TIGR01847; HMM-score: 11)
  • TheSEED:
  • PFAM:
    no clan definedAIRS_C; AIR synthase related protein, C-terminal domain (PF02769; HMM-score: 129.6)
    AIRS; AIR synthase related protein, N-terminal domain (PF00586; HMM-score: 59.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.141
    • Ymax_pos: 43
    • Cmax: 0.115
    • Cmax_pos: 5
    • Smax: 0.259
    • Smax_pos: 39
    • Smean: 0.109
    • D: 0.129
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 445952957 NCBI
  • RefSeq: WP_000030812 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGATFDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYFLDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGAVEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVFLEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIFDWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNESTAIELLGV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS05260 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]