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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01015
  • pan locus tag?: SAUPAN003284000
  • symbol: SAOUHSC_01015
  • pan gene symbol?: purM
  • synonym:
  • product: phosphoribosylaminoimidazole synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01015
  • symbol: SAOUHSC_01015
  • product: phosphoribosylaminoimidazole synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 986197..987225
  • length: 1029
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCTAAAGCATATGAACAATCTGGTGTAAATATTCATGCTGGTTATGAAGCTGTAGAA
    AGAATGTCTAGTCATGTTAAACGTACGATGCGTAAAGAAGTTATCGGTGGTTTAGGTGGA
    TTCGGTGCTACATTTGATTTATCACAATTAAATATGACAGCGCCAGTTTTAGTTTCTGGA
    ACAGACGGAGTAGGTACGAAATTAAAACTAGCTATCGACTATGGTAAACATGATTCGATA
    GGTATCGATGCAGTCGCAATGTGTGTTAATGATATTTTAACGACAGGTGCAGAACCATTA
    TATTTTTTAGATTACATTGCTACAAACAAAGTTGTTCCTGAAGTTATTGAACAAATTGTT
    AAAGGTATTAGTGATGCATGTGTTGAAACGAATACTGCACTTATCGGTGGAGAGACTGCT
    GAAATGGGTGAAATGTATCACGAAGGCGAATATGATGTAGCTGGATTTGCTGTTGGAGCA
    GTTGAAAAGGATGACTATGTAGATGGTTCAGAAGTGAAAGAGGGACAAGTTGTTATAGGG
    CTTGCGTCAAGTGGCATTCATTCAAATGGATACAGTTTAGTGCGCAAATTAATTAATGAA
    TCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAA
    CCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCA
    ATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGA
    TATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAATATTTGATTGGTTACAA
    CAACAAGGCAATATAGACACAAATGAAATGTATAACATTTTTAATATGGGTATTGGCTAT
    ACGGTTATCGTTGATGAAAAAGATGTATCACGCGCTTTGAAGATTTTAGCAGAACAAAAT
    GTGGAAGCCTATCAAATTGGTCATATTGTGAAAAATGAGTCAACTGCAATTGAATTGTTG
    GGGGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1029

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01015
  • symbol: SAOUHSC_01015
  • description: phosphoribosylaminoimidazole synthetase
  • length: 342
  • theoretical pI: 4.52335
  • theoretical MW: 37016.8
  • GRAVY: -0.0175439

Function[edit source | edit]

  • reaction:
    EC 6.3.3.1?  ExPASy
    Phosphoribosylformylglycinamidine cyclo-ligaseATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylformylglycinamidine cyclo-ligase (TIGR00878; EC 6.3.3.1; HMM-score: 429.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiamine-phosphate kinase (TIGR01379; EC 2.7.4.16; HMM-score: 41.6)
    hydrogenase expression/formation protein HypE (TIGR02124; HMM-score: 41.3)
    MetabolismEnergy metabolismMethanogenesisputative methanogenesis marker protein 2 (TIGR03267; HMM-score: 32.6)
    selenide, water dikinase (TIGR00476; EC 2.7.9.3; HMM-score: 24.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylformylglycinamidine synthase II (TIGR01736; EC 6.3.5.3; HMM-score: 22.2)
    Unknown functionEnzymes of unknown specificityAIR synthase-related protein, sll0787 family (TIGR04049; HMM-score: 17.6)
    Unknown functionEnzymes of unknown specificityAIR synthase-related protein, MSMEG_0567 C-terminal family (TIGR04050; HMM-score: 17.3)
    bacteriocin-type signal sequence (TIGR01847; HMM-score: 11)
  • TheSEED:  
    Nucleosides and NucleotidesPurinesDe Novo Purine Biosynthesis Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1) 
  • PFAM:
    no clan definedAIRS_C; AIR synthase related protein, C-terminal domain (PF02769; HMM-score: 129.6)
    AIRS; AIR synthase related protein, N-terminal domain (PF00586; HMM-score: 59.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.141
    • Ymax_pos: 43
    • Cmax: 0.115
    • Cmax_pos: 5
    • Smax: 0.259
    • Smax_pos: 39
    • Smean: 0.109
    • D: 0.129
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGATFDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYFLDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGAVEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVFLEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIFDWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNESTAIELLGV

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]