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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01013
  • pan locus tag?: SAUPAN003282000
  • symbol: SAOUHSC_01013
  • pan gene symbol?: purL
  • synonym:
  • product: phosphoribosylformylglycinamidine synthase II

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01013
  • symbol: SAOUHSC_01013
  • product: phosphoribosylformylglycinamidine synthase II
  • replicon: chromosome
  • strand: +
  • coordinates: 982552..984741
  • length: 2190
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCTAAATTTATCGAACCAAGCGTTGAAGAAATTAAACTTGAAAAAGTATATCAAGAT
    ATGGGATTAAGTGATCAAGAATATGAAAAAGTTTGCGATATTTTAGGCAGACAACCTAAC
    TTTACAGAAACAGGTATCTTTTCTGTTATGTGGAGTGAACATTGCTCTTATAAACATTCT
    AAACCGTTTTTAAAGCAATTTCCTACGTCAGGTGACCATGTGCTTATGGGGCCTGGTGAA
    GGTGCAGGGGTAGTCGATATAGGTGATAATCAAGCCGTAGTATTTAAAGTAGAGTCTCAC
    AATCATCCATCAGCAATTGAACCATATCAAGGGGCTGCTACAGGCGTTGGTGGAATCATT
    CGTGACATTGTCTCTATTGGGGCTAGACCTATTAATTTGTTAAACAGTCTTAGATTTGGA
    GAATTAGATAATAAACAAAACCAAAGATTACTTAAAGGTGTTGTAAAGGGTATCGGAGGT
    TATGGTAACTGCATTGGTATTCCAACAACTGCTGGTGAAATCGAATTTGATGAACGTTAT
    GATGGCAATCCACTTGTTAATGCAATGTGTGTTGGTGTTATCAATCACGACATGATTCAA
    AAAGGCACAGCAAAAGGTGTAGGTAATTCGGTCATTTATGTTGGTTTGAAAACTGGTCGA
    GATGGTATTCATGGTGCTACTTTTGCATCTGAAGAATTGACGGAAGAAAGCGAAAGTAAA
    CGACCTTCTGTACAAATCGGTGATCCATTTGTAGGTAAAAAATTAATGGAAGCAACACTT
    GAAGCAATTACATTTGATGAATTAGTTGGTATTCAAGATATGGGTGCTGCTGGTTTAACA
    TCTTCATCGTCTGAAATGGCGGCAAAAGGTGGTAGTGGGTTACATTTGAGATTAGAACAA
    GTGCCAACACGTGAGCCAGGTATTTCTCCTTATGAAATGATGCTTTCAGAAACTCAAGAA
    CGTATGTTACTAGTTGTTGAAAAAGGTACTGAACAAAAATTCTTAGATTTATTTGATAAG
    CACGAATTGGATAGTGCTGTTATAGGTGAAGTTACAGATACAAATCGTTTTGTTTTAACA
    TATGATGACGAAGTTTATGCTGACATTCCAGTTGAACCACTAGCTGATGAAGCACCTGTA
    TATATTTTAGAAGGAGAAGAAAAAGATTATAATACTTCTAAAAATGATTATACACACATC
    GATGTTAAAGATACTTTCTTTAAATTACTTAAGCATCCGACTATAGCATCTAAACACTAT
    TTATATGATCAATACGACCAACAAGTTGGTGCCAATACGATAATTAAGCCAGGACTTCAA
    GCATCGGTAGTACGTGTGGAAGGCACAAATAAGGCAATTGCTTCAACAATTGATGGTGAA
    GCGCGTTATGTATATAACAATCCATATGAAGGTGGAAAGATGGTAGTAGCTGAAGCTTAT
    CGAAATTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGT
    TCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAA
    GCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTTATATAACGAAACG
    AAAGGTACTTCTATTTTCCCAACACCAGTTGTTGGAATGGTAGGTTTGATTGAAAATGTA
    AATTATTTAAATGATTTTGAACCTCAAGTTGGAGATAAATTATATTTAATCGGTGATACT
    AAGGACGACTTTGGTGGTAGTCAACTTGAAAAGTTAATTTATGGCAAAGTTAATCATGAA
    TTTGAGTCATTAGATTTGAGTTCAGAAGTTGAAAAAGGTGAATCAATCAAGACCGCTATT
    CGTGAAGGACTATTATCACATGTTCAAACAGTTGGTAAAGGTGGCTTACTGATTACCTTA
    GCTAAACTAAGTGCGCATTACGGTTTAGGATTAAAATCTTCAATAGATATAACAAATGCA
    CAATTGTTTAGTGAGACGCAAGGCCGATATGTTGTTTCTGTTAAATCAGGTAAAACTTTA
    AATATTGATAATGCAATAGAAATTGGACTTTTAACAGATAGTGATAATTTCAAGGTAACA
    ACACCATATACAGAGATTAGTGAAAATGTTTCAGATATTAAACAAATATGGGAAGGGGCA
    ATTGCTCAATGTTTAACTACTCAGGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2190

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01013
  • symbol: SAOUHSC_01013
  • description: phosphoribosylformylglycinamidine synthase II
  • length: 729
  • theoretical pI: 4.50802
  • theoretical MW: 79535.2
  • GRAVY: -0.253086

Function[edit source | edit]

  • reaction:
    EC 6.3.5.3?  ExPASy
    Phosphoribosylformylglycinamidine synthaseATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylformylglycinamidine synthase II (TIGR01736; EC 6.3.5.3; HMM-score: 1004.7)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylformylglycinamidine synthase (TIGR01857; EC 6.3.5.3; HMM-score: 166.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylformylglycinamidine synthase (TIGR01735; EC 6.3.5.3; HMM-score: 139)
    MetabolismEnergy metabolismMethanogenesisputative methanogenesis marker protein 2 (TIGR03267; HMM-score: 64.6)
    hydrogenase expression/formation protein HypE (TIGR02124; HMM-score: 41.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiamine-phosphate kinase (TIGR01379; EC 2.7.4.16; HMM-score: 37.9)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylformylglycinamidine cyclo-ligase (TIGR00878; EC 6.3.3.1; HMM-score: 37.1)
    Unknown functionEnzymes of unknown specificityAIR synthase-related protein, sll0787 family (TIGR04049; HMM-score: 35.9)
    herpesvirus tegument protein/v-FGAM-synthase (TIGR01739; HMM-score: 12.6)
  • TheSEED:  
    Nucleosides and NucleotidesPurinesDe Novo Purine Biosynthesis Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) 
  • PFAM:
    no clan definedAIRS; AIR synthase related protein, N-terminal domain (PF00586; HMM-score: 157.8)
    AIRS_C; AIR synthase related protein, C-terminal domain (PF02769; HMM-score: 131.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.101
    • Ymax_pos: 30
    • Cmax: 0.111
    • Cmax_pos: 61
    • Smax: 0.137
    • Smax_pos: 19
    • Smean: 0.086
    • D: 0.095
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTETGIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNHPSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGGYGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKTGRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGAAGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKFLDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNTSKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTNKAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIYQQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDFEPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGLLSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNIDNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]