NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS05960 [old locus tag: NWMN_1055 ]
• pan locus tag^?: SAUPAN003381000
• symbol: NWMN_RS05960
• pan gene symbol^?: polX
• synonym:
• product: DNA polymerase/3'-5' exonuclease PolX

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS05960 [old locus tag: NWMN_1055 ]
• symbol: NWMN_RS05960
• product: DNA polymerase/3'-5' exonuclease PolX
• replicon: chromosome
• strand: +
• coordinates: 1157536..1159248
• length: 1713
• essential: unknown

⊟Accession numbers

• Location: NC_009641 (1157536..1159248) NCBI
• MicrobesOnline:

⊟Phenotype

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⊟DNA sequence

• 1
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  1561
  1621
  1681
  
  ATGACAAAAAAAGATGTTATCAAACTATTAGAACAAATTGCTACTTATATGGAATTAAAA
  GGGGAAAATACTTTTAAAATATCAGCGTATCGAAAAGCAGCTCAAAGTCTTGAATTAGAT
  GAACGACCATTAGATGAAATATCTGATGTAACGGAGTTAAAAGGCATTGGTAAAGGTGTT
  GCAGAAGTAATCAATGATTACCGTGAGACCGGTGAATCTCAGTATTTACAGCAATTACAG
  GAAGAAGTTCCGGAAGGTCTTATTCCACTTTTGAAAATTCAAGGACTTGGAAGCAAGAAA
  ATTGCTAAGCTATATAAAGAGTTGAATATTGTTGATAAAGCGTCACTTCAAGTTGCTTGT
  GAAAATGGAAAAGTTAGTGAATTAAGCGGATTTGCTAAGAAAACGGAACAAAACATATTA
  GAAGCTGTGAAACAACTTGGTGCTAAGAAAGATAGATATCCAATTGATCAAATGAGAAGA
  CTTAATCAAGAAATCATTGATTATATAGATACATTAAATTATATCGATCAATATTCATCT
  GCAGGAAGCTTCCGTCGTTTTAAAGAAATGAGCAAAGATTTAGATTTCATAATAAGTACC
  GATAACCCAAAAGCAGTGCAGCAGCAATTATTAAATATTCCCAATAAAGTAAAAGAAGTT
  GCAGTGGGGAACACAAAAGTTTCATTAGAATTAGCGTATGATGATGAAACGATTGGTGTC
  GATTTTCGATTAATTGAACCAAGTGCTTTTTATCATACATTGCAGCATTTTACTGGGTCA
  AAAGAACATAATATAAGAATTCGACAACTTGCTAAAGCACGTGATGAAAAAGTAAGTGAA
  TATGGAATTGAACAAGCTGATGGTACATTAATTCAATATGATAGTGAAGCCAAGATATAT
  GAACATTTTAATGTGAATTTTATACCACCTGCTATGCGAGAAGATGGTAGCGAATTTGAT
  AAAGATCTAAGTAATATCATTACAATAGATGATATTAATGGTGATATTCATATGCATACA
  ACGTATAGTGATGGTGCGTTTTCTATTCGAGACATGGTAGAAGCAAATATCGCAAAAGGT
  TATAAATTCATGGTAATTACTGATCATTCACAAAGTTTACGTGTTGCTAATGGCTTACAA
  GTAGAAAGACTTTTAAGACAAAACGAAGAAATTAAGGCTTTAGATAAAGAATATAGTGAA
  ATTGATATTTATTCAGGTACAGAAATGGATATATTACCTGATGGCTCGCTGGATTATGAT
  GATGAAATTTTAGCACAACTTGATTATGTAATTGGAGCTATTCATCAAAGCTTTAACCAA
  TCAGAAGAACAAATTATGGAACGATTAGCTAATGCATGTCGCAATCCATACGTGCGACAT
  ATAGCGCATCCAACAGGGCGTATTATAGGTAGAAGAGATGGTTATAAACCGAATATTGAA
  CAATTAATGGCATTAGCTGAAGAAACGAATACAGTATTAGAAATTAATGCCAATCCACAT
  CGACTGGATCTGAGCGCTGATATCGTTCGTAAATATCCAAATGTGAAATTAACTATTAAC
  ACTGATGCGCATCATACAAATCATTTAGATTTTATGAATTATGGCGTAGCAACTGCGCAA
  AAAGGATTTGTAACAAAAGATAGAGTGATTAACGCATTATCGCGTGAAGCTTTTAAAGAC
  TTTATTGAAAATAATACAAAACTTAAGAAATAG
  60
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  1560
  1620
  1680
  1713
  

⊟Protein

⊟General

• locus tag: NWMN_RS05960 [old locus tag: NWMN_1055 ]
• symbol: NWMN_RS05960
• description: DNA polymerase/3'-5' exonuclease PolX
• length: 570
• theoretical pI: 5.01717
• theoretical MW: 64869.8
• GRAVY: -0.51807

⊟Function

• reaction:
• TIGRFAM:
  histidinol phosphate phosphatase, HisJ family (TIGR01856; HMM-score: 37.9)
  Cellular processesCellular processesDNA transformationcomEA protein (TIGR01259; HMM-score: 11.6)
  Genetic information processingDNA metabolismDNA replication, recombination, and repairHolliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 9.5)
• TheSEED:
• PFAM:
  HHH (CL0198) HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 72.6)
  no clan definedDNA_pol_B_thumb; DNA polymerase beta thumb (PF14791; HMM-score: 58.9)
  Amidohydrolase (CL0034) PHP; PHP domain (PF02811; HMM-score: 33.3)
  HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 25)
  NTP_transf (CL0260) DNA_pol_B_palm; DNA polymerase beta palm (PF14792; HMM-score: 21.3)
  HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 15.8)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • Ymax: 0.1
  • Ymax_pos: 37
  • Cmax: 0.11
  • Cmax_pos: 37
  • Smax: 0.14
  • Smax_pos: 9
  • Smean: 0.092
  • D: 0.097
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446084087 NCBI
• RefSeq: WP_000161942 NCBI
• UniProt:

⊟Protein sequence

• MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPLDEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIAKLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRRLNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVKEVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDEKVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDINGDIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIKALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLANACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADIVRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNTKLKK

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• NWMN_RS05960 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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