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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS06165 [old locus tag: NWMN_1087 ]
  • pan locus tag?: SAUPAN003446000
  • symbol: NWMN_RS06165
  • pan gene symbol?: bshC
  • synonym:
  • product: bacillithiol biosynthesis cysteine-adding enzyme BshC

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS06165 [old locus tag: NWMN_1087 ]
  • symbol: NWMN_RS06165
  • product: bacillithiol biosynthesis cysteine-adding enzyme BshC
  • replicon: chromosome
  • strand: +
  • coordinates: 1190296..1191909
  • length: 1614
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1190296..1191909) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
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    1021
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    1561
    ATGGACTGTAAAGTAGTTAGTTTAAATGAAAAAGATCAGTTTATACCAAAAATAAAGAGC
    AGTGACCCTGTAATAACAGGATTATTTCAATATGATGCAGCTCAACAAACTAGTTTTGAA
    AAAAGGATGTCTAAAGAAAATAATGGAAGAGAAGCGGCATTAGCGAATGTTATTCGTGAA
    TATATGAGTGATTTAAAGCTTTCAAGTGAACAAGAATTAAACATACAACATTTAGCTAAT
    GGTTCAAAAGTTGTGATTGGTGGACAACAAGCAGGGCTTTTCGGGGGACCATTGTATACA
    TTCCATAAAATATTTTCAATCATTACTTTATCTAAGGAATTAACGGATACACATAAGCAA
    CAAGTAGTACCAGTTTTTTGGATTGCAGGAGAAGATCATGATTTCGATGAAGTGAATCAT
    ACATTTGTTTATAACGAAAATCATGGGTCGCTGCATAAGGTTAAATATCATACAATGGAG
    ATGCCAGAGACGACTGTCTCTAGATATTATCCTGATAAGGCTGAGTTGAAACAAACTTTA
    AAAACGATGTTCATTCATATGAAAGAAACTGTTCATACACAAGGTCTACTGGAGATTTGT
    GACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAA
    ACATTTAAAGCATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATG
    GAAGCGCCTATGTTTAAAAAGATTTTGAAAAAACATCAGTTGCTTGATGATGCTTTTAGA
    GCAACACAACAACGTACTCAAAATCAAGGCTTGAATGCGATGATACAAACAGATACAAAT
    GTTCATTTATTCTTACATGATGAAAATATGCGTCAATTAGTTTCGTATGATGGTAAGCAT
    TTTAAATTAAATAAAACAGATAAGACATATATAAAGGAAGAAATTATAAATATTGCGGAA
    AATCAACCTGAATTATTTTCTAATAATGTAGTGACAAGACCATTAATGGAAGAATGGTTA
    TTTAACACGGTGGCATTTGTTGGAGGACCGAGTGAAATTAAGTACTGGGCTGAACTAAAA
    GATGTATTTGAACTATTTGATGTTGAAATGCCTATCGTGATGCCAAGGCTTAGAATTACT
    TATTTAAATGACCGTATAGAAAAATTACTTTCGAAATACAATATTCCATTAGAAAAAGTG
    TTAGTCGATGGTGTTGAAGGAGAAAGAAGTAAGTTTATTAGAGAACAAGCATCACATCAA
    TTTATTGAAAAGGTAGAAGGTATGATTGAACAACAGCGTCGTCTAAACAAAGACTTATTA
    GATGAAGTGGCGGGGAATCAAAATAATATTAACCTTGTGAATAAAAATAATGAAATTCAT
    ATACAACAGTATGATTATTTGTTAAAACGTTATCTTTTAAACATTGAAAGAGAAAACGAC
    ATCAGTATGAAGCAATTTAGAGAAATTCAAGAAACACTCCATCCAATGGGAGGATTACAA
    GAAAGAATATGGAATCCACTTCAAATTTTGAATGATTTTGGGACAGATGTGTTCAAGCCC
    TCCACCTATCCACCACTTTCTTACACTTTTGATCATATTATTATAAAACCTTAA
    60
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    1614

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS06165 [old locus tag: NWMN_1087 ]
  • symbol: NWMN_RS06165
  • description: bacillithiol biosynthesis cysteine-adding enzyme BshC
  • length: 537
  • theoretical pI: 6.12147
  • theoretical MW: 62806.4
  • GRAVY: -0.489199

Function[edit source | edit]

  • reaction:
    EC 6.-.-.-?  ExPASy
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsbacillithiol biosynthesis cysteine-adding enzyme BshC (TIGR03998; HMM-score: 627.8)
  • TheSEED:
  • PFAM:
    HUP (CL0039) BshC; Bacillithiol biosynthesis BshC (PF10079; HMM-score: 523.9)
    no clan definedMinor_capsid_1; Minor capsid protein (PF10665; HMM-score: 13.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.155
    • Ymax_pos: 43
    • Cmax: 0.131
    • Cmax_pos: 43
    • Smax: 0.34
    • Smax_pos: 33
    • Smean: 0.142
    • D: 0.15
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446262620 NCBI
  • RefSeq: WP_000340475 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMSKENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFHKIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTMEMPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALLHETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQTDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRPLMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSKYNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNINLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQILNDFGTDVFKPSTYPPLSYTFDHIIIKP

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS06165 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]