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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07235 [old locus tag: NWMN_1284 ]
  • pan locus tag?: SAUPAN003767000
  • symbol: NWMN_RS07235
  • pan gene symbol?: trpB
  • synonym:
  • product: tryptophan synthase subunit beta

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS07235 [old locus tag: NWMN_1284 ]
  • symbol: NWMN_RS07235
  • product: tryptophan synthase subunit beta
  • replicon: chromosome
  • strand: +
  • coordinates: 1414774..1415988
  • length: 1215
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (1414774..1415988) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1284

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATAAACAAATACAAACAGAAGCAGATGAATTAGGTTTCTTTGGTGAATATGGAGGG
    CAATATGTTCCAGAAACATTAATGCCAGCAATTATTGAGTTGAAAAAAGCTTATAAAGAG
    GCAAAAGCAGACCCAGAGTTTCAAAGAGAACTGGAATACTATTTATCAGAGTATGTAGGA
    CGCGCGACACCACTTACATATGCTGCATCATATACTGAAAGCCTAGGTGGCGCTAAAATA
    TATTTGAAACGAGAGGATCTAAATCATACAGGCGCCCATAAAATTAATAATGCGTTAGGT
    CAAGCGTTGCTTGCTAAAAGAATGGGCAAGAAGAAGCTTGTTGCTGAAACTGGTGCGGGT
    CAACATGGTGTAGCTAGTGCTACGGTTGCTGCATTATTTGATATGGAACTTGTTGTCTTT
    ATGGGAAGTGAAGATATTAAAAGACAACAACTTAATGTATTTAGAATGGAATTACTTGGT
    GCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCA
    TTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCATTAGGT
    CCAGACCCGTTCCCAACGATTGTTAGAGATTTTCAGAGTGTGATTGGTAAAGAAATAAAA
    TCACAGATATTGAAGAAAGAAGGTCGACTTCCGGATGCAATTGTAGCATGTATCGGTGGT
    GGCTCAAATGCAATCGGTACATTTTATCCATTTATTAAAGATGATGTTGCATTATACGGT
    GTTGAAGCCGCAGGTCAAGGCGATGATACTGATAAACATGCACTTGCAATTGGCAAAGGA
    TCACCTGGCGTATTACATGGTACTAAAATGTATTTAATTCAAGATGAAGATGGGCAAGTG
    CAACTAGCACATTCTATTTCAGCAGGACTTGATTATCCTGGTATTGGACCAGAACATTCT
    TATTACCACGACATTGGTAGAGTAACTTTTGAAAATGCTAGTGATACACAAGCAATGAAT
    GCTTTAATCAACTTTACAAAACATGAAGGTATTATACCTGCAATTGAAAGTGCACATGCA
    CTGAGTTATGTTGAAAGACTAGCGCCTACGATGTCGAAAGAAGATATTATTGTAGTAACT
    ATTTCTGGACGTGGCGATAAAGATATGGAAACAATTAGACAATATATGGTAGAGCGAGGT
    CTTGCAAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1215

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS07235 [old locus tag: NWMN_1284 ]
  • symbol: NWMN_RS07235
  • description: tryptophan synthase subunit beta
  • length: 404
  • theoretical pI: 5.07529
  • theoretical MW: 43964.5
  • GRAVY: -0.223762

Function[edit | edit source]

  • reaction:
    EC 4.2.1.20?  ExPASy
    Tryptophan synthase L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 650.9)
    and 11 more
    Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 181.4)
    Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 44.3)
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 32.1)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 30.6)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 30)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 28.5)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 28)
    ectoine utilization protein EutB (TIGR02991; HMM-score: 26.7)
    Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 24.4)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 16)
    pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family (TIGR01275; HMM-score: 14.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 134.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00892
    • TAT(Tat/SPI): 0.000876
    • LIPO(Sec/SPII): 0.001005
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKADPEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQALLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLGAKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKEIKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALAIGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENASDTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETIRQYMVERGLAND

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]