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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07235 [old locus tag: NWMN_1284 ]
  • pan locus tag?: SAUPAN003767000
  • symbol: NWMN_RS07235
  • pan gene symbol?: trpB
  • synonym:
  • product: tryptophan synthase subunit beta

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS07235 [old locus tag: NWMN_1284 ]
  • symbol: NWMN_RS07235
  • product: tryptophan synthase subunit beta
  • replicon: chromosome
  • strand: +
  • coordinates: 1414774..1415988
  • length: 1215
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1414774..1415988) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATAAACAAATACAAACAGAAGCAGATGAATTAGGTTTCTTTGGTGAATATGGAGGG
    CAATATGTTCCAGAAACATTAATGCCAGCAATTATTGAGTTGAAAAAAGCTTATAAAGAG
    GCAAAAGCAGACCCAGAGTTTCAAAGAGAACTGGAATACTATTTATCAGAGTATGTAGGA
    CGCGCGACACCACTTACATATGCTGCATCATATACTGAAAGCCTAGGTGGCGCTAAAATA
    TATTTGAAACGAGAGGATCTAAATCATACAGGCGCCCATAAAATTAATAATGCGTTAGGT
    CAAGCGTTGCTTGCTAAAAGAATGGGCAAGAAGAAGCTTGTTGCTGAAACTGGTGCGGGT
    CAACATGGTGTAGCTAGTGCTACGGTTGCTGCATTATTTGATATGGAACTTGTTGTCTTT
    ATGGGAAGTGAAGATATTAAAAGACAACAACTTAATGTATTTAGAATGGAATTACTTGGT
    GCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCA
    TTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCATTAGGT
    CCAGACCCGTTCCCAACGATTGTTAGAGATTTTCAGAGTGTGATTGGTAAAGAAATAAAA
    TCACAGATATTGAAGAAAGAAGGTCGACTTCCGGATGCAATTGTAGCATGTATCGGTGGT
    GGCTCAAATGCAATCGGTACATTTTATCCATTTATTAAAGATGATGTTGCATTATACGGT
    GTTGAAGCCGCAGGTCAAGGCGATGATACTGATAAACATGCACTTGCAATTGGCAAAGGA
    TCACCTGGCGTATTACATGGTACTAAAATGTATTTAATTCAAGATGAAGATGGGCAAGTG
    CAACTAGCACATTCTATTTCAGCAGGACTTGATTATCCTGGTATTGGACCAGAACATTCT
    TATTACCACGACATTGGTAGAGTAACTTTTGAAAATGCTAGTGATACACAAGCAATGAAT
    GCTTTAATCAACTTTACAAAACATGAAGGTATTATACCTGCAATTGAAAGTGCACATGCA
    CTGAGTTATGTTGAAAGACTAGCGCCTACGATGTCGAAAGAAGATATTATTGTAGTAACT
    ATTTCTGGACGTGGCGATAAAGATATGGAAACAATTAGACAATATATGGTAGAGCGAGGT
    CTTGCAAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1215

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS07235 [old locus tag: NWMN_1284 ]
  • symbol: NWMN_RS07235
  • description: tryptophan synthase subunit beta
  • length: 404
  • theoretical pI: 5.07529
  • theoretical MW: 43964.5
  • GRAVY: -0.223762

Function[edit source | edit]

  • reaction:
    EC 4.2.1.20?  ExPASy
    Tryptophan synthaseL-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAromatic amino acid familytryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 650.9)
    Unknown functionEnzymes of unknown specificitypyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 181.4)
    MetabolismAmino acid biosynthesisPyruvate familythreonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 44.3)
    Cellular processesCellular processesBiosynthesis of natural products2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 32.1)
    MetabolismAmino acid biosynthesisSerine familycysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 30.6)
    MetabolismAmino acid biosynthesisSerine familycysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 30)
    MetabolismAmino acid biosynthesisSerine familycysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 28.5)
    MetabolismAmino acid biosynthesisPyruvate familythreonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 28)
    ectoine utilization protein EutB (TIGR02991; HMM-score: 26.7)
    MetabolismAmino acid biosynthesisAspartate familythreonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 24.4)
    MetabolismAmino acid biosynthesisPyruvate familythreonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 16)
    pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family (TIGR01275; HMM-score: 14.6)
  • TheSEED:
  • PFAM:
    no clan definedPALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 134.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.128
    • Ymax_pos: 44
    • Cmax: 0.132
    • Cmax_pos: 44
    • Smax: 0.181
    • Smax_pos: 39
    • Smean: 0.079
    • D: 0.109
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446964081 NCBI
  • RefSeq: WP_001041337 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKADPEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQALLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLGAKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKEIKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALAIGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENASDTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETIRQYMVERGLAND

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS07235 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]