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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07680 [old locus tag: NWMN_1363 ]
  • pan locus tag?: SAUPAN003912000
  • symbol: NWMN_RS07680
  • pan gene symbol?: nth
  • synonym:
  • product: endonuclease III

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS07680 [old locus tag: NWMN_1363 ]
  • symbol: NWMN_RS07680
  • product: endonuclease III
  • replicon: chromosome
  • strand: -
  • coordinates: 1527492..1528151
  • length: 660
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1527492..1528151) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTAAGTAAGAAAAAAGCATTAGAAATGATTGATGTTATAGCAAATATGTTTCCGGAT
    GCAGAATGTGAATTAAAACATGATAATCCGTTCGAATTAACTATTGCTGTATTATTGTCA
    GCGCAATGTACAGACGTTCTAGTAAATAGAGTAACAACTGAGCTGTTTAAAAAATACAAA
    ACGCCTGAAGATTATTTGGCAGTCAGTGATGAAGAACTTATGAATGATATTCGTTCTATC
    GGTCTGTATCGTAACAAAGCTAAGAATATTAAGAAACTTTGTCAATCTTTGATTGATCAA
    TTTAATGGAGAAATACCACAAACACATAAGGAATTAGAAAGTTTAGCAGGTGTAGGACGT
    AAAACTGCTAATGTAGTCATGAGTGTAGCATTTGATGAACCTTCATTAGCTGTTGATACG
    CATGTAGAACGTGTTTCTAAACGCTTAGGTATTAATCGTTGGAAAGATAATGTGAGACAG
    GTAGAAGACCGTTTATGTTCTGTCATACCTAGAGATAGATGGAATAGGAGCCATCATCAA
    CTCATTTTCTTTGGAAGATACCACTGTTTAGCCCGAAAACCTAAATGCGATATTTGTCCA
    CTATTAGAAGATTGTAGAGAAGGACAAAAACGTTATAAAGCTAGTTTGAAAGAAGCGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS07680 [old locus tag: NWMN_1363 ]
  • symbol: NWMN_RS07680
  • description: endonuclease III
  • length: 219
  • theoretical pI: 8.39452
  • theoretical MW: 25234
  • GRAVY: -0.456621

Function[edit source | edit]

  • reaction:
    EC 4.2.99.18?  ExPASy
    DNA-(apurinic or apyrimidinic site) lyaseThe C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairendonuclease III (TIGR01083; EC 4.2.99.18; HMM-score: 273.8)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairA/G-specific adenine glycosylase (TIGR01084; EC 3.2.2.-; HMM-score: 89)
    Genetic information processingDNA metabolismDNA replication, recombination, and repair8-oxoguanine DNA-glycosylase (ogg) (TIGR00588; EC 3.2.2.-; HMM-score: 21.6)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIIA (TIGR01662; HMM-score: 14)
  • TheSEED:
  • PFAM:
    HHH (CL0198) HhH-GPD; HhH-GPD superfamily base excision DNA repair protein (PF00730; HMM-score: 82.1)
    HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 33.7)
    no clan definedEndIII_4Fe-2S; Iron-sulfur binding domain of endonuclease III (PF10576; HMM-score: 23.9)
    DUF2400; Protein of unknown function (DUF2400) (PF09674; HMM-score: 12.6)
    P-loop_NTPase (CL0023) AAA_32; AAA domain (PF13654; HMM-score: 11.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: [4Fe-4S] cluster
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.145
    • Ymax_pos: 24
    • Cmax: 0.119
    • Cmax_pos: 49
    • Smax: 0.289
    • Smax_pos: 1
    • Smean: 0.171
    • D: 0.155
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446175910 NCBI
  • RefSeq: WP_000253765 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCTDVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFNGEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQVEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKEA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS07680 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]