From AureoWiki
Jump to: navigation, search
NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08065 [old locus tag: NWMN_1428 ]
  • pan locus tag?: SAUPAN004078000
  • symbol: NWMN_RS08065
  • pan gene symbol?: xseA
  • synonym:
  • product: exodeoxyribonuclease 7 large subunit

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS08065 [old locus tag: NWMN_1428 ]
  • symbol: NWMN_RS08065
  • product: exodeoxyribonuclease 7 large subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 1599947..1601284
  • length: 1338
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1599947..1601284) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCAGATTATTTAAGTGTTTCAGCTTTAACGAAATATATTAAATATAAATTTGATCAA
    GATCCACATCTCCAATCTGTATTGATTAAAGGTGAGCTTTCAAATTTCAAAAAGCATTCA
    AGTGGTCATTTATACTTTAATGTGAAAGATAAAGAAAGCGTTATAAGTGCCATGATGTTC
    AAAGGTAGTGCTTCTAAATTAAACTTCGAACCTAAAGAAGGAGATGAAGTCTTATTAGAA
    GCACGTGTTTCTGTTTTTGAACGTCGTGGAAACTATCAAATTTATGTAAATAAAATGCAA
    TTAGATGGTATAGGGAACTTATATCAAAAATTAGAAGCATTAAAGAAAAAATTAACTGAA
    GAAGGTTGTTTTGATAAAGCGAATAAGAAATCAATACCTAAGTTTCCTAAAAAAATTGCA
    GTTTTAACAGCGAGTACAGGTGCCGCAATTCGAGATATCCATTCAACGATAAATAGTCGT
    TTTCCATTAGCTGAACAAATACAAATTAGTACTTTGGTTCAAGGTGAAAAAGCAAAAGAC
    GACATTATTGAAAAAATTGAATATGCAGATAGTTTAGGTGTAGATACCATTATTGTAGGT
    CGAGGCGGTGGATCCATAGAAGATTTATGGAATTTCAACGAAGAAGCTGTCGTCCGTGCA
    ATATATAATTGTAAGACACCGATTATATCAGCAGTTGGTCATGAAACAGACTTTACATTA
    AGTGATTTTGCTGCAGACATCAGAGCTGCGACTCCAACTCAAGCTGCTGTTATTGCAACA
    CCAGACCAATATGAATTGCTGCAACAAATACAGCAATATCAGTTTACATTGACTCGTTTC
    ATCAAGAAACATTTAGAACAACAACGTAAACATGTTGAACATTTGTCATCGTATTACAAG
    TTTAAACAACCAACTTTATTGTATGATCAACAGATACAACGTCGAGATGATTTAGAAAAG
    AGACTGAAACAACAAATTCAGGCAACTTTTGAACAACAGAGACATCGATTAATGCTGTTG
    CAACAACGTTATAATTTAAAAGCATTACTAAGTAGTGTTAATCAAGAACAACAAAATAAT
    CTTCAATTGACAAACCAACTTGTTAAATTATTGAATTCTAAGATATTAAGTTATAAAAAT
    GATTTGAAAAATAAAGTAGAAAACTTAAACAATTTAAGTCCAACTAATACAATGTTGCGT
    GGATATGCAATTGTTAATAAAAAAGACGAAGTCATTACGAGTACTAAAGATTTAACAGAA
    AATGATCAATTGACGTTAACAATGAAAGATGGCTTAGTAGATGCAAAAGTTACGAAAGTA
    AGGTGTAATAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1338

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS08065 [old locus tag: NWMN_1428 ]
  • symbol: NWMN_RS08065
  • description: exodeoxyribonuclease 7 large subunit
  • length: 445
  • theoretical pI: 9.48419
  • theoretical MW: 50893.8
  • GRAVY: -0.52809

Function[edit source | edit]

  • reaction:
    EC 3.1.11.6?  ExPASy
    Exodeoxyribonuclease VIIExonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates
  • TIGRFAM:
    Genetic information processingDNA metabolismDegradation of DNAexodeoxyribonuclease VII, large subunit (TIGR00237; EC 3.1.11.6; HMM-score: 489)
  • TheSEED:
  • PFAM:
    no clan definedExonuc_VII_L; Exonuclease VII, large subunit (PF02601; HMM-score: 311.8)
    OB (CL0021) tRNA_anti_2; OB-fold nucleic acid binding domain (PF13742; HMM-score: 96.8)
    tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 34.4)
    Cache (CL0165) dCache_1; Cache domain (PF02743; HMM-score: 16.6)
    TIM_barrel (CL0036) AP_endonuc_2; Xylose isomerase-like TIM barrel (PF01261; HMM-score: 12.3)
    PepSY_TM-like (CL0490) PepSY_TM_like_2; Putative PepSY_TM-like (PF16357; HMM-score: 11.8)
    no clan definedTransglut_i_TM; Inactive transglutaminase fused to 7 transmembrane helices (PF14400; HMM-score: 11.3)
    DUF4279; Domain of unknown function (DUF4279) (PF14106; HMM-score: 10.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.122
    • Ymax_pos: 43
    • Cmax: 0.121
    • Cmax_pos: 43
    • Smax: 0.195
    • Smax_pos: 38
    • Smean: 0.109
    • D: 0.117
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447209672 NCBI
  • RefSeq: WP_001286928 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS08065 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]