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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08755 [old locus tag: NWMN_1561 ]
  • pan locus tag?: SAUPAN004282000
  • symbol: NWMN_RS08755
  • pan gene symbol?: hemL
  • synonym:
  • product: glutamate-1-semialdehyde 2,1-aminomutase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS08755 [old locus tag: NWMN_1561 ]
  • symbol: NWMN_RS08755
  • product: glutamate-1-semialdehyde 2,1-aminomutase
  • replicon: chromosome
  • strand: -
  • coordinates: 1727560..1728846
  • length: 1287
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1727560..1728846) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGATATACGAAATCAGAAGAAGCAATGAAGGTTGCTGAAACTTTAATGCCTGGTGGT
    GTAAATAGTCCAGTACGCGCATTTAAATCAGTAGATACACCAGCAATTTTTATGGATCAC
    GGTAAAGGTTCAAAAATTTATGATATCGATGGTAACGAGTATATCGACTATGTACTAAGT
    TGGGGACCACTTATTTTAGGACATAGAGACCCTCAAGTTATTAGTCATTTACATGAAGCA
    ATTGATAAAGGTACAAGTTTTGGTGCATCAACATTACTTGAAAATAAATTGGCGCAGCTC
    GTTATTGACCGAGTACCTTCAATAGAAAAAGTGCGTATGGTGTCATCTGGTACAGAAGCT
    ACATTGGATACTTTAAGATTAGCACGTGGTTATACTGGCAGAAATAAAATTGTGAAATTT
    GAAGGTTGCTATCATGGTCATAGTGATTCGTTATTAATCAAAGCTGGTTCTGGGGTGGCA
    ACATTAGGATTGCCGGATTCTCCTGGTGTGCCTGAAGGTATTGCTAAAAATACAATTACA
    GTTCCATACAATGATTTAGATGCACTTAAAATCGCTTTCGAAAAATTTGGAAACGATATT
    GCTGGTGTAATCGTAGAACCTGTTGCTGGTAATATGGGTGTCGTACCGCCGATTGAAGGT
    TTTTTACAGGGATTAAGAGATATTACGACTGAATACGGCGCATTGCTAATTTTCGATGAA
    GTAATGACTGGTTTCAGAGTCGGTTATCATTGTGCACAAGGTTACTTTGGTGTGACACCA
    GATTTAACTTGCTTAGGAAAAGTTATCGGTGGAGGACTACCTGTAGGTGCATTTGGTGGT
    AAAAAAGAAATCATGGATCATATAGCACCATTAGGAAATATTTATCAAGCGGGTACGTTA
    TCAGGAAATCCTCTTGCAATGACAAGTGGTTATGAAACGTTAAGCCAATTAACGCCAGAG
    ACATATGAGTATTTTAATATGTTAGGCGATATACTTGAAGACGGTTTAAAACGTGTATTT
    GCTAAACACAATGTACCAATAACTGTAAATAGAGCAGGTTCAATGATTGGTTATTTCTTA
    AATGAAGGACCTGTAACTAATTTTGAACAAGCGAATAAAAGTGATTTGAAATTATTTGCA
    GAAATGTATCGAGAAATGGCAAAAGAAGGTGTGTTTTTACCACCATCTCAATTTGAAGGT
    ACATTCTTATCTACGGCACACACGAAAGAAGATATTGAAAAAACGATTCAAGCATTTGAT
    ACGGCTTTAAGTCGTATTGTAAAATAA
    60
    120
    180
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    540
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    720
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    840
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    1020
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    1140
    1200
    1260
    1287

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS08755 [old locus tag: NWMN_1561 ]
  • symbol: NWMN_RS08755
  • description: glutamate-1-semialdehyde 2,1-aminomutase
  • length: 428
  • theoretical pI: 5.29503
  • theoretical MW: 46387.8
  • GRAVY: -0.0616822

Function[edit source | edit]

  • reaction:
    EC 5.4.3.8?  ExPASy
    Glutamate-1-semialdehyde 2,1-aminomutase(S)-4-amino-5-oxopentanoate = 5-aminolevulinate
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminglutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 624.6)
    transaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 179)
    MetabolismEnergy metabolismAmino acids and aminessuccinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 143.1)
    ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 134.9)
    MetabolismCentral intermediary metabolismOther4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 132.6)
    MetabolismCentral intermediary metabolismOther2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 125.3)
    MetabolismCentral intermediary metabolismPolyamine biosynthesisputrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 121.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinadenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 120.8)
    Cellular processesCellular processesAdaptations to atypical conditionsdiaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 89.9)
    L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 46.7)
    MetabolismCentral intermediary metabolismOther4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 29.1)
  • TheSEED:
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 246.9)
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 16.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.146
    • Ymax_pos: 11
    • Cmax: 0.139
    • Cmax_pos: 28
    • Smax: 0.316
    • Smax_pos: 2
    • Smean: 0.199
    • D: 0.167
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447193612 NCBI
  • RefSeq: WP_001270868 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGSKIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVIDRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVATLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPIEGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVGAFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILEDGLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVFLPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS08755 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]