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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS09310 [old locus tag: NWMN_1658 ]
  • pan locus tag?: SAUPAN004454000
  • symbol: NWMN_RS09310
  • pan gene symbol?: putA
  • synonym:
  • product: proline dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS09310 [old locus tag: NWMN_1658 ]
  • symbol: NWMN_RS09310
  • product: proline dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1854327..1855328
  • length: 1002
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1854327..1855328) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCACTATTAAAGAATTTTTTTATCGGATTATCTAATAATAGTTTTTTAAACAACGCA
    GCAAAAAAAGTGGGCCCACGTTTGGGCGCCAATAAAGTCGTTGCCGGAAATACAATTCCA
    GAGTTAATTAATACAATCGAATACTTAAATGACAAGAATATCGCTGTTACGGTAGACAAT
    TTAGGGGAATTTGTCGGTACAGTTGAAGAAAGTAATCATGCTAAAGAACAAATTTTAACA
    ATTATGGACGCGCTTCATCAACATGGCGTAAAGGCACATATGTCTGTTAAATTGAGTCAG
    TTAGGTGCAGAATTCGACTTAGAATTAGCTTACCAAAATTTAAGAGAGATTTTACTTAAA
    GCAAATACTTACAACAATATGCATATAAATATTGATACTGAAAAATATGCTAGCCTGCAA
    CAAATTGTTCAAGTTTTAGATCGCTTAAAAGGCGAATTTAGAAATGTTGGTACTGTAATT
    CAAGCATATTTATACGATAGCCACGAATTAGTTGATAAGTACCAAGATTTACGATTACGT
    TTGGTTAAAGGTGCATATAAAGAAAACGAATCAATTGCATTTCAATCTAAGGAAGACGTA
    GATGCAAATTACATCAAAATAATTGAACAACGTTTGTTAAACGCACGCAATTTCACTTCA
    ATTGCAACACATGACCATCGCATCATTAATCATGTAAAACAATTTATGAAAGAAAATCAC
    ATTGAAAAAGATCGTATGGAATTCCAAATGCTCTATGGTTTTAGATCAGAGTTAGCAGAA
    GAAATCGCAAATGAAGGCTATAATTTCACTATTTATGTACCTTATGGCGATGATTGGTTT
    GCGTATTTTATGAGAAGATTAGCAGAACGCCCACAAAACCTATCTCTTGCTGTAAAAGAA
    TTTGTGAAACCTGCTGGCTTAAAACGTGTTGGCATAATTGCAGCTTTAGGAGCTACAGTT
    ATGTTAGGTTTAAGTACAATTAAAAAATTATGCCGTAAATAG
    60
    120
    180
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    1002

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS09310 [old locus tag: NWMN_1658 ]
  • symbol: NWMN_RS09310
  • description: proline dehydrogenase
  • length: 333
  • theoretical pI: 8.36497
  • theoretical MW: 38029.5
  • GRAVY: -0.244144

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    acidobacterial duplicated orphan permease (TIGR03434; HMM-score: 15.5)
  • TheSEED:
  • PFAM:
    FAD_oxidored (CL0086) Pro_dh; Proline dehydrogenase (PF01619; HMM-score: 109.5)
    no clan definedPorB; Alpha helical Porin B (PF11565; HMM-score: 13.7)
    DUF3347; Protein of unknown function (DUF3347) (PF11827; HMM-score: 13)
    ExbD; Biopolymer transport protein ExbD/TolR (PF02472; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.275
    • Ymax_pos: 21
    • Cmax: 0.158
    • Cmax_pos: 21
    • Smax: 0.555
    • Smax_pos: 19
    • Smean: 0.446
    • D: 0.342
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

  • GI: 447119098 NCBI
  • RefSeq: WP_001196354 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELINTIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLGAEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVIQAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNFTSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYGDDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS09310 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]