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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS09650 [old locus tag: NWMN_1719 ]
  • pan locus tag?: SAUPAN004592000
  • symbol: NWMN_RS09650
  • pan gene symbol?: lukE
  • synonym:
  • product: gamma-hemolysin subunit A

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS09650 [old locus tag: NWMN_1719 ]
  • symbol: NWMN_RS09650
  • product: gamma-hemolysin subunit A
  • replicon: chromosome
  • strand: -
  • coordinates: 1916843..1917778
  • length: 936
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1916843..1917778) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTTAAGAAAAAAATGTTAGCTGCAACTTTGTCAGTAGGACTGATTGCACCTTTAGCA
    TCTCCGATTCAAGAATCTAGAGCAAATACTAATATTGAAAATATTGGTGATGGTGCTGAA
    GTAATCAAACGTACGGAGGATGTAAGTAGTAAGAAATGGGGCGTTACTCAAAATGTCCAA
    TTCGACTTTGTAAAAGATAAAAAATATAACAAAGACGCTTTAATTGTTAAAATGCAAGGT
    TTTATTAATTCCAGAACTTCATTTTCAGATGTGAAGGGTAGTGGATATGAATTAACTAAA
    CGAATGATTTGGCCATTCCAATATAATATAGGACTGACGACTAAAGATCCAAATGTTAGC
    TTAATCAATTACCTTCCTAAAAACAAAATAGAAACTACTGATGTTGGTCAAACATTAGGA
    TATAACATTGGAGGTAATTTCCAGTCAGCACCATCTATAGGTGGCAATGGCTCATTTAAT
    TATTCTAAAACAATTAGTTATACCCAAAAGAGTTATGTCAGTGAAGTAGACAAGCAAAAC
    TCAAAATCTGTTAAATGGGGTGTTAAAGCAAACGAATTTGTTACGCCTGATGGAAAAAAA
    TCTGCGCATGATAGATATTTATTCGTACAAAGTCCAAATGGTCCAACAGGTTCAGCAAGA
    GAATATTTTGCTCCTGATAATCAATTGCCACCTTTAGTTCAAAGTGGCTTTAATCCATCG
    TTTATCACTACACTATCACATGAAAAAGGTTCAAGTGATACGAGTGAATTTGAAATTTCA
    TATGGTAGAAACTTAGATATTACATATGCGACTTTATTCCCTAGAACTGGTATTTACGCA
    GAAAGAAAGCATAATGCATTTGTAAATAGAAACTTTGTAGTTAGATATGAAGTTAATTGG
    AAAACACACGAAATTAAAGTGAAAGGACATAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS09650 [old locus tag: NWMN_1719 ]
  • symbol: NWMN_RS09650
  • description: gamma-hemolysin subunit A
  • length: 311
  • theoretical pI: 9.98997
  • theoretical MW: 34818.9
  • GRAVY: -0.582315

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesToxin production and resistancebeta-channel forming cytolysin (TIGR01002; HMM-score: 305.4)
  • TheSEED:
  • PFAM:
    Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 287.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide length 29 aa
    • Ymax: 0.562
    • Ymax_pos: 29
    • Cmax: 0.428
    • Cmax_pos: 29
    • Smax: 0.942
    • Smax_pos: 19
    • Smean: 0.824
    • D: 0.664
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446395741 NCBI
  • RefSeq: WP_000473596 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKRTEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRMIWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFNYSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGSAREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRTGIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS09650 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]