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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS10545 [old locus tag: NWMN_1835 ]
  • pan locus tag?: SAUPAN004921000
  • symbol: NWMN_RS10545
  • pan gene symbol?: rumA
  • synonym:
  • product: RNA methyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS10545 [old locus tag: NWMN_1835 ]
  • symbol: NWMN_RS10545
  • product: RNA methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2041451..2042812
  • length: 1362
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2041451..2042812) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    361
    421
    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    TTGCAAGCAATAGCTAAAAATGACATAAAAACGGGAACAGTTGTTGATTTAACGCATGAA
    GGCCATGGTGTCGTAAAAATAGATCGCTTTCCTATTTTCATACCACAAGCTTTAATTAAT
    GAACAAATTGAATATAAAATTATCAAAGTAAAAAAGAACTTTGCGATAGGTAAGTTGCTT
    AATATAAATACGAGAAGTGAGAATCGTGTTGCACCGCCATGTATATATTATGAACGTTGT
    GGTGGGTGCCAACTTCAACACTTATCATACGAAGCACAACTTGAAATGAAAAAGGAACAA
    GTGATTAATTTGTTTCAGCGAAAAGCGCATTTTGATAATTCTAAGATTAACGATACAGTT
    GGCATGACAGATCCTTGGAGATATCGAAACAAGTCCCAGATTCCAGTTGGTAAGAATGAA
    CAGAATGAAGTTATTATGGGCTTTTATCGTCAAAGAAGCCATGACATTATTGACATGGAA
    AGTTGTTTAATACAAGATTCACAACATCAAGAAGTGATGAATGAAGTTAAATCTATTTTA
    AAAGATTTAAATGTAAGTATTTATCAAGAACAATTGAAAAAAGGTTTAATGAGACATTTG
    GTTGTAAGAACAGGATATCATACAGACGAAATGATGATTATTTTTGTAACTAATGGTAAA
    AAGTGGCCACAAAAAAATGCTGTTGTTGAGAAGATATTAGATGCATTTCCTAATGTGACA
    AGCATTAAACAAAACATTAATGATAGCCACTCTAATGTTATTATGGGACGTCAATCTATA
    ACGCTATATGGTAAAGATACAATTATTGATCAATTAACAGATAGTACATTTAAAATAAGT
    GATCAGTCATTCTACCAAATTAATTCTGAACAAACAGAGAAATTATATAATAAAGCAATT
    GAGTATGCGCAATTGACAGGTAATGAAGTTGTATTAGATACCTATTGTGGTATTGGAACA
    ATAGGGCTATATATGGCACCACTTGCAAAACATGTATACGGTGTTGAAGTAGTACCTTCT
    GCAATTGAAGACGCACAACAAAATGCGACTATAAACCAATGCAATAATACAACTTTTGTC
    TGTGGGAAAGCTGAAGAAGTTATTTTACAATGGAAAGCACAAGGGATTAAACCAGATGTT
    GTAATGGTTGATCCGCCAAGAAAGGGCTGTGACGAAACGTTTATTCAGACTTTGCTTACA
    TTAGAACCTAAGCGTATTGTCTATATTTCATGTAATCCCGCAACACAGCAACGTGATGCA
    TTATTATTAGCTGAAAAATATCAACTTGAAGAGGTAACACCGGTAGATATGTTTCCACAA
    ACAACACATGTTGAGACAGTGGCATTATTCAATTTGAAATAG
    60
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    1362

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS10545 [old locus tag: NWMN_1835 ]
  • symbol: NWMN_RS10545
  • description: RNA methyltransferase
  • length: 453
  • theoretical pI: 6.73139
  • theoretical MW: 51682
  • GRAVY: -0.34702

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modification23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 527.8)
    Genetic information processingProtein synthesistRNA and rRNA base modification23S rRNA (uracil-5-)-methyltransferase RumB (TIGR02085; EC 2.1.1.189; HMM-score: 173.8)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA (uracil(54)-C(5))-methyltransferase (TIGR02143; EC 2.1.1.35; HMM-score: 84.7)
    Genetic information processingProtein fateProtein modification and repairprotein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 32.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllmagnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 22.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprecorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 21.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 21.2)
    Genetic information processingProtein fateProtein modification and repairmethyltransferase, HemK family (TIGR00536; HMM-score: 20.1)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 18.7)
    Unknown functionEnzymes of unknown specificityputative methylase (TIGR00537; HMM-score: 17.4)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationputative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 15.1)
    Genetic information processingProtein synthesistRNA and rRNA base modification16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (TIGR00095; EC 2.1.1.171; HMM-score: 13.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsputative 4-mercaptohistidine N1-methyltranferase (TIGR04345; HMM-score: 13.6)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 13.5)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) tRNA_U5-meth_tr; tRNA (Uracil-5-)-methyltransferase (PF05958; HMM-score: 84.9)
    Met_10; Met-10+ like-protein (PF02475; HMM-score: 32.9)
    Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 31.2)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 30.1)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 28.9)
    Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 26)
    PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 22.1)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 22)
    N6_N4_Mtase; DNA methylase (PF01555; HMM-score: 19.5)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 17.6)
    Cystine-knot (CL0079) PDGF; PDGF/VEGF domain (PF00341; HMM-score: 16.3)
    OB (CL0021) TRAM; TRAM domain (PF01938; HMM-score: 15.2)
    NADP_Rossmann (CL0063) Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 14.9)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 13.1)
    TPMT; Thiopurine S-methyltransferase (TPMT) (PF05724; HMM-score: 11.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.103
    • Ymax_pos: 11
    • Cmax: 0.112
    • Cmax_pos: 28
    • Smax: 0.157
    • Smax_pos: 1
    • Smean: 0.092
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447070612 NCBI
  • RefSeq: WP_001147868 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIEYKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVINLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDMESCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTNGKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDSTFKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYGVEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDETFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALFNLK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS10545 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]