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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS11430 [old locus tag: NWMN_1975 ]
  • pan locus tag?: SAUPAN005341000
  • symbol: NWMN_RS11430
  • pan gene symbol?: alr
  • synonym:
  • product: alanine racemase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS11430 [old locus tag: NWMN_1975 ]
  • symbol: NWMN_RS11430
  • product: alanine racemase
  • replicon: chromosome
  • strand: -
  • coordinates: 2193212..2194360
  • length: 1149
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2193212..2194360) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTCGGATAAATATTATAGATCTGCGTATATGAATGTAGATTTAAACGCTGTTGCATCA
    AATTTCAAAGTATTCAGTACATTGCATCCAAATAAAACAGTGATGGCTGTCGTTAAAGCC
    AATGCCTATGGACTAGGTAGTGTTAAAGTAGCACGTCATTTAATGGAAAATGGCGCCACA
    TTTTTTGCTGTAGCAACGTTAGATGAAGCGATAGAACTTAGAATGCATGGGATTACTGCT
    AAAATTTTAGTCTTAGGTGTGTTACCAGCTAAAGATATTGATAAAGCGATACAACACCGA
    GTTGCCTTAACGGTTCCGTCTAAACAGTGGTTGAAAGAAGCAATTAAAAACATTTCTGGT
    GAGCAAGAGAAAAAGTTATGGTTGCACATTAAATTAGATACAGGAATGGGACGTTTAGGT
    ATTAAAGATACTAAAACGTATCAAGAAGTGATTGAAATCATTCAACAATATGAGCAACTT
    GTATTTGAAGGCGTGTTTACACACTTTGCCTGTGCTGACGAACCAGGAGATATGACAACT
    GAACAATATCAACGTTTTAAAGATATGGTCAATGAAGCAATTAAACCTGAATATATACAT
    TGTCAGAACTCAGCAGGCTCTCTATTAATGGATTGCCAATTCTGTAATGCAATAAGACCA
    GGAATTTCCCTTTATGGATATTATCCATCAGAGTATGTACAGCAAAAAGTTAAAGTACAC
    CTTAAACCAAGTGTGCAATTAATTGCTAATGTAGTTCAAACAAAGACGCTACAAGCGGGT
    GAGTCTGTAAGTTATGGTGCAACTTATACAGCTACTGACCCAACTACAATAGCATTGTTA
    CCTATTGGATATGCAGATGGCTATTTACGCATAATGCAAGGTAGCTTTGTAAATGTAAAT
    GGTCATCAATGCGAAGTTATTGGTCGCGTATGTATGGATCAGACAATTGTTAAAGTGCCA
    GATCAAGTTAAAGCTGGAGATTCGGTGATTTTAATAGATAATCATAGAGAAAGTCCACAG
    TCGGTAGAGGTGGTAGCTGAAAAGCAACATACTATTAATTATGAAGTGCTTTGTAACTTG
    TCGAGACGTTTGCCGCGAATCTATCATGATGGTGATCAACGTTTTGTAACAAATGAATTG
    TTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1149

Protein[edit source | edit]

Protein Data Bank: 3OO2

General[edit source | edit]

  • locus tag: NWMN_RS11430 [old locus tag: NWMN_1975 ]
  • symbol: NWMN_RS11430
  • description: alanine racemase
  • length: 382
  • theoretical pI: 7.33759
  • theoretical MW: 42823
  • GRAVY: -0.158377

Function[edit source | edit]

  • reaction:
    EC 5.1.1.1?  ExPASy
    Alanine racemaseL-alanine = D-alanine
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanalanine racemase (TIGR00492; EC 5.1.1.1; HMM-score: 373.1)
    PRTRC system protein B (TIGR03737; HMM-score: 12.2)
  • TheSEED:
  • PFAM:
    TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 223.5)
    no clan definedAla_racemase_C; Alanine racemase, C-terminal domain (PF00842; HMM-score: 143.5)
    Styelin; Styelin A-E (PF17562; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.89
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.283
    • Ymax_pos: 37
    • Cmax: 0.234
    • Cmax_pos: 37
    • Smax: 0.448
    • Smax_pos: 23
    • Smean: 0.293
    • D: 0.287
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447203889 NCBI
  • RefSeq: WP_001281145 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYGLGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVALTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQLVFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAIRPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTTIALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDNHRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS11430 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]