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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS11460 [old locus tag: NWMN_1981 ]
  • pan locus tag?: SAUPAN005348000
  • symbol: NWMN_RS11460
  • pan gene symbol?: kdpB
  • synonym:
  • product: potassium-transporting ATPase subunit B

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS11460 [old locus tag: NWMN_1981 ]
  • symbol: NWMN_RS11460
  • product: potassium-transporting ATPase subunit B
  • replicon: chromosome
  • strand: -
  • coordinates: 2198111..2200138
  • length: 2028
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2198111..2200138) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCATCATGTAAATAAATATTTCAATCAAACAATGGTGATAGAAGCTTTGAAAATGAGT
    TTTTATAAGCTGAATCTTAAACAATTAATAAAAAATCCGATAATGTTTGTCGTTGAGGTT
    GGAATGATTTTAACGTTGATATTAATATGTTTTCCTGACATATTTGGAACTAGCTATCTA
    TCGCGAGGTTATTTGATTACAATTTTTATCATATTATTAATAACTATATTGTTTGCTAAT
    TTTTCAGAAGCCTTTGCTGAAGGTCGTGGAAAAGCGCAAGCTGATAGTTTAAGACAAGCA
    CAATCAAATTTAACTGCTCGATTGATAGAAGAAAATGGTGCGTATCGGATAGTGAACGCC
    ACAGAGTTAAAAGCCGGACAAAATATTCGGGTAGAAAATGGAGAAACAATCCCTGCTGAT
    GGCGTTGTAATAAATGGATTAGCAACCGTTGATGAATCTGCAATTACTGGTGAGTCTGCA
    CCTGTGATAAAAGAATCAGGTGGCGATTTTGATGGAGTCATTGGCGGTACACTTGTAACA
    TCTGATTGGTTAGAAATACGTGTTGAGAGTGAAGCGGGTACATCTTTTTTAGATAAAATG
    ATTGCCTTAGTTGAAGGGGCGGAAAGAAATAAAACACCAAATGAAATTGCACTTTTTACA
    TTATTAACAACGTTAACAATTATATTTTTAGTCGTTATTGTAACGTTATATCCAATAGCA
    TCTTACCTGCATTTAATTTTACCTATTGCGATGCTCATTGCGTTAACAGTATGTTTAATA
    CCAACGACAATCGGTGGTTTGTTATCGGCGATTGGTATTGCAGGCATGGATCGTGTTACT
    CAATTCAATGTATTAGCGAAAAGTGGGCGTGCTGTAGAAGTATGTGGAGATGTCGATGTA
    ATGATTTTAGACAAGACAGGCACAATCACTTATGGCAATCGAATCGCTAGTGAATTTTTA
    CCAGTGAATCAACAAATGCTTGAAAAATTGATAGTCGCTGCTTATATGTCATCAATCTAC
    GATGATACGCCAGAAGGAAAAAGTATTGTACGATTAGCGAAGCAGATGTATATAAATGAG
    CTGCCTAAAGACATTGATGGCACATATAAGCCTTTTACAGCTGAAACTAGAATGAGCGGA
    ATCATTACAAATGAAATATCGGTATTCAAAGGTGCACCTAATAGTATGATAAATCTTGTC
    AAACAACAACAAGGCAATATTCCTCTTAATATTGAGTCTCTCTGTATGGATGTATCGAGT
    AAAGGTGGCACACCATTAATAGTCATTGAAAACAATGTCATGCTTGGCGTGATTTATTTA
    AAAGATGTGATTAAAGATGGTCTTGTAGAACGATTTACTGAATTGAGAAAAATGGGGATT
    GAAACAGTTATGTGTACAGGTGATAATGCTTTAACAGCAGCTACAATTGCAAAAGAAGCC
    GGGGTTGATAGATTTGTTGCAGAGTGTAAACCCGAAGATAAAATTAAAGTCATCAAAGAT
    GAACAAGCGAAAGGCCATATTGTTGCGATGACAGGAGATGGTACAAATGATGCACCTGCG
    CTAGCACAAGCGAATATAGGATTAGCTATGAACTCAGGGACAATTAGTGCTAAAGAGGCA
    GCTAATCTAATTGATTTAGATTCGAATCCTACAAAGTTAATAGAGGTTGTAAAAATAGGC
    AAACAATTATTAATGACGAGAGGTGCATTAACGACATTTAGTTTGGCTAATGACGTAGCA
    AAATACTTTGCAATTTTACCTGCACTAATGATGTCAACAATTCCAGAAATGACTTCACTA
    AATATTATGCATTTATCGTCACCTAAATCTGCAATTATATCAGCTTTGATATTCAATGCA
    TTAATCATTGTTGCCTTAATTCCAATTGCCATGAAAGGTGTAAAAGTAAAGGGTTACAGC
    ATTGATAGAATCTTTATAAATAATATGTTGATTTATGGATTAGGTGGATTAATCGTCCCG
    TTTTTAGGCATTAAATTAATAGATATGATTGTACAATTTTTTGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2028

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS11460 [old locus tag: NWMN_1981 ]
  • symbol: NWMN_RS11460
  • description: potassium-transporting ATPase subunit B
  • length: 675
  • theoretical pI: 5.09826
  • theoretical MW: 73152.7
  • GRAVY: 0.426667

Function[edit source | edit]

  • reaction:
    EC 3.6.3.12?  ExPASy
    Potassium-transporting ATPaseATP + H2O + K+(Out) = ADP + phosphate + K+(In)
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsK+-transporting ATPase, B subunit (TIGR01497; EC 3.6.3.12; HMM-score: 987.7)
    heavy metal translocating P-type ATPase (TIGR01525; EC 3.6.3.-; HMM-score: 296.5)
    HAD ATPase, P-type, family IC (TIGR01494; EC 3.6.3.-; HMM-score: 262.4)
    MetabolismTransport and binding proteinsCations and iron carrying compoundscadmium-translocating P-type ATPase (TIGR01512; EC 3.6.3.3; HMM-score: 219.5)
    Cellular processesCellular processesDetoxificationcopper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 217.9)
    MetabolismTransport and binding proteinsCations and iron carrying compoundscopper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 217.9)
    plasma-membrane proton-efflux P-type ATPase (TIGR01647; EC 3.6.3.6; HMM-score: 162.2)
    calcium-translocating P-type ATPase, PMCA-type (TIGR01517; EC 3.6.3.8; HMM-score: 120.2)
    MetabolismTransport and binding proteinsCations and iron carrying compoundscalcium-translocating P-type ATPase, SERCA-type (TIGR01116; EC 3.6.3.8; HMM-score: 102.5)
    calcium-transporting P-type ATPase, PMR1-type (TIGR01522; EC 3.6.3.8; HMM-score: 100.6)
    P-type ATPase of unknown pump specificity (type V) (TIGR01657; HMM-score: 100)
    MetabolismEnergy metabolismATP-proton motive force interconversionNa,H/K antiporter P-type ATPase, alpha subunit (TIGR01106; EC 3.6.3.-; HMM-score: 92.8)
    potassium/sodium efflux P-type ATPase, fungal-type (TIGR01523; EC 3.6.3.-; HMM-score: 87.3)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmagnesium-translocating P-type ATPase (TIGR01524; EC 3.6.3.2; HMM-score: 82.8)
    Unknown functionEnzymes of unknown specificityCof-like hydrolase (TIGR00099; HMM-score: 25.1)
    phospholipid-translocating P-type ATPase, flippase (TIGR01652; EC 3.6.3.1; HMM-score: 22.1)
    MetabolismAmino acid biosynthesisSerine familyphosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 20.3)
    Unknown functionEnzymes of unknown specificityHAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 18.4)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 13.2)
  • TheSEED:
  • PFAM:
    HAD (CL0137) Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 109.5)
    no clan definedE1-E2_ATPase; E1-E2 ATPase (PF00122; HMM-score: 108.6)
    HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 26.7)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 14.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.103
    • Ymax_pos: 11
    • Cmax: 0.121
    • Cmax_pos: 57
    • Smax: 0.143
    • Smax_pos: 3
    • Smean: 0.106
    • D: 0.105
  • predicted transmembrane helices (TMHMM): 7

Accession numbers[edit source | edit]

  • GI: 446468730 NCBI
  • RefSeq: WP_000546584 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMILTLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQSNLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESAPVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIALFTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGMDRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAAYMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGAPNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVERFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIVAMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMTRGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIVALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS11460 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]