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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS14005 [old locus tag: NWMN_2437 ]
  • pan locus tag?: SAUPAN006195000
  • symbol: NWMN_RS14005
  • pan gene symbol?: glcB
  • synonym:
  • product: PTS glucoside EIICBA component

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS14005 [old locus tag: NWMN_2437 ]
  • symbol: NWMN_RS14005
  • product: PTS glucoside EIICBA component
  • replicon: chromosome
  • strand: -
  • coordinates: 2678317..2680383
  • length: 2067
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2678317..2680383) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    1021
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    2041
    ATGTTTAAGAAATTGTTTGGACAATTGCAACGTATCGGTAAAGCATTAATGTTACCTGTT
    GCGATTTTACCAGCAGCTGGTATTTTATTAGCGTTTGGTAACGCAATGCACAACGAACAA
    TTAGTAGAAATTGCACCATGGTTAAAAAACGATATCATTGTAATGATTTCGTCGGTCATG
    GAAGCAGCAGGACAAGTTGTATTTGATAACTTGCCATTATTATTTGCAGTTGGTACAGCA
    CTTGGATTAGCAGGAGGAGACGGTGTTGCAGCATTAGCAGCGCTAGTAGGTTACTTAATT
    ATGAATGCAACAATGGGGAAAGTGTTGCACATTACAATTGATGACATTTTCTCATATGCC
    AAAGGGGCAAAAGAATTAAGTCAAGCAGCGAAAGAACCAGCACATGCTTTAGTATTAGGT
    ATTCCAACGTTACAAACGGGTGTGTTTGGTGGTATTATCATGGGTGCTTTAGCCGCATGG
    TGTTACAACAAATTTTATAATATTACACTACCACCATTTTTAGGATTCTTTGCAGGTAAA
    CGATTTGTACCGATTGTGACATCGGTCGTAGCAATCGCAACAGGTGTGCTTTTAAGCTTT
    GCGTGGCCACCAATTCAAGATGGATTAAATAGTTTATCGAATTTCTTATTAAATAAAAAT
    TTAACATTAACAACGTTTATATTCGGTATTATTGAACGCTCATTAATTCCATTTGGTTTA
    CATCATATTTTCTATTCACCGTTCTGGTTTGAATTCGGAAGTTATACAAATCACGCAGGT
    GAATTGGTTCGTGGTGACCAACGTATTTGGATGGCACAATTGAAAGATGGCGTACCATTT
    ACTGCTGGTGCATTTACTACTGGTAAATATCCATTTATGATGTTTGGTTTACCAGCGGCG
    GCATTTGCTATTTATAAAAATGCACGACCAGAACGTAAAAAAGTCGTGGGTGGTTTAATG
    TTATCAGCAGGATTAACTGCATTTTTAACTGGTATCACTGAGCCATTAGAATTTTCATTC
    TTATTTGTAGCACCAGTACTTTATGGAATTCACGTATTATTAGCTGGTACATCATTCTTA
    GTAATGCATTTATTAGGCGTTAAAATTGGTATGACATTCTCAGGTGGTTTCATAGATTAT
    ATTTTATATGGTTTATTAAACTGGGATCGTTCACACGCATTATTAGTTATTCCAGTCGGT
    ATTGTATATGCTATCGTGTATTACTTCTTATTCGACTTTGCAATTCGTAAGTTTAAATTG
    AAAACACCAGGTCGTGAAGATGAAGAAACTGAAATTCGTAACTCTAGTGTCGCAAAATTA
    CCATTTGATGTCTTAGATGCAATGGGTGGAAAAGAAAACATTAAACATTTAGATGCATGT
    ATTACACGTCTACGCGTAGAAGTGGTTGATAAATCAAAAGTAGATGTAGCAGGTATTAAA
    GCTTTAGGCGCATCAGGTGTATTAGAAGTTGGAAACAATATGCAAGCTATCTTTGGTCCA
    AAATCAGATCAAATTAAACATGATATGGCCAAGATTATGAGTGGTGAAATTACGAAACCA
    AGTGAAACGACAGTGACTGAAGAAATGTCAGATGAACCAGTTCACGTAGAAGCACTTGGA
    ACAACAGACATCTATGCACCAGGTATCGGTCAAATCATTCCATTATCAGAAGTACCTGAT
    CAAGTATTCGCTGGTAAAATGATGGGTGATGGTGTTGGCTTTATCCCTGAAAAAGGTGAA
    ATTGTAGCACCGTTTGATGGTACAGTGAAAACAATCTTCCCTACGAAACATGCGATAGGA
    TTAGAATCTGAAAGTGGCGTCGAAGTACTTATTCATATTGGTATCGATACAGTGAAACTG
    AATGGTGAAGGATTCGAAAGTCTGATTAACGTTGATGAAAAAGTAACACAAGGTCAACCA
    TTAATGAAAGTGAATTTAGCATACTTGAAAGCACACGCACCAAGCATCGTTACACCAATG
    ATTATTACAAATCTTGAAAATAAAGAACTTGTCATTGAAGATGTACAAGATGCTGATCCA
    GGTAAGCTAATTATGACAGTCAAATAA
    60
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    2067

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS14005 [old locus tag: NWMN_2437 ]
  • symbol: NWMN_RS14005
  • description: PTS glucoside EIICBA component
  • length: 688
  • theoretical pI: 6.13064
  • theoretical MW: 74415.8
  • GRAVY: 0.418895

Function[edit source | edit]

  • reaction:
    EC 2.7.1.-?  ExPASy
    Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine?
    Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate?
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 849.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 451.7)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 380.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 303.5)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 277)
    Signal transductionSignal transductionPTSPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 277)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 256.4)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 189.2)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 145.2)
    Signal transductionSignal transductionPTSPTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 145.2)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 70.9)
    Signal transductionSignal transductionPTSPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 70.9)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 53.6)
    PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 28.6)
  • TheSEED:
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 320)
    Hybrid (CL0105) PTS_EIIA_1; phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1 (PF00358; HMM-score: 168.3)
    no clan definedPTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 54.3)
    Hybrid (CL0105) RnfC_N; RnfC Barrel sandwich hybrid domain (PF13375; HMM-score: 18.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.227
    • Ymax_pos: 15
    • Cmax: 0.154
    • Cmax_pos: 38
    • Smax: 0.619
    • Smax_pos: 9
    • Smean: 0.479
    • D: 0.325
  • predicted transmembrane helices (TMHMM): 9

Accession numbers[edit source | edit]

  • GI: 446395820 NCBI
  • RefSeq: WP_000473675 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEIAPWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMNATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAWCYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLNKNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKDGVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITEPLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSHALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGGKENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHDMAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGKMMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEGFESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGKLIMTVK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS14005 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]