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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS14065 [old locus tag: NWMN_2447 ]
  • pan locus tag?: SAUPAN006209000
  • symbol: NWMN_RS14065
  • pan gene symbol?:
  • synonym:
  • product: methylated-DNA--[protein]-cysteine S-methyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS14065 [old locus tag: NWMN_2447 ]
  • symbol: NWMN_RS14065
  • product: methylated-DNA--[protein]-cysteine S-methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2690307..2690828
  • length: 522
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2690307..2690828) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGAGTATAAGAGTTACTATGATTCGCCTGTAGGACGATTGGAACTTTTGAGTGATGGG
    GTAAGTCTGACCGCTGTTTTGTTTGAAAATCAACAAGGTGATGGTACTAGGGAAGAAAAT
    ACGTCTTTAGCGATATTTAGAGAGGCGACACAGTGGTTAGACGCGTATTTTAAAGGTGAC
    AATCCTGAAATCACAATACCTTTAAAGCCAACAGGTAGTCATTTTCAACAGTGTGTTTGG
    AATGAATTAAGACAGGTTCCTTATGGGACTCTAACAACGTATGGTGCTATTGCCAAAAAA
    GTAGGCAAGCTGCTTAACAAGCCAAAAATGTCAGCCCAAGCGGTTGGAGGTGCAGTCGGA
    AGTAATCCATTATCTATTATCGTGCCATGTCATCGTGTTGTCGGTAAAACGGGTAGCTTA
    ACAGGGTTCGGTGGAACGATTAATAATAAAATTAAGCTGTTAGAGTTGGAGAATATCGAT
    ATGTCAAAGCTTTATGTACCAAAACATAGCACGAAGCCGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    522

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS14065 [old locus tag: NWMN_2447 ]
  • symbol: NWMN_RS14065
  • description: methylated-DNA--[protein]-cysteine S-methyltransferase
  • length: 173
  • theoretical pI: 9.01352
  • theoretical MW: 18945.6
  • GRAVY: -0.29422

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairmethylated-DNA--[protein]-cysteine S-methyltransferase (TIGR00589; EC 2.1.1.63; HMM-score: 92.5)
  • TheSEED:
  • PFAM:
    no clan definedDNA_binding_1; 6-O-methylguanine DNA methyltransferase, DNA binding domain (PF01035; HMM-score: 103.1)
    Methyltransf_1N; 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain (PF02870; HMM-score: 30.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.161
    • Ymax_pos: 38
    • Cmax: 0.14
    • Cmax_pos: 38
    • Smax: 0.3
    • Smax_pos: 24
    • Smean: 0.152
    • D: 0.157
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446378132 NCBI
  • RefSeq: WP_000455987 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLAIFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVGKLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENIDMSKLYVPKHSTKP

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS14065 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]