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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS14380 [old locus tag: NWMN_2506 ]
  • pan locus tag?: SAUPAN006309000
  • symbol: NWMN_RS14380
  • pan gene symbol?:
  • synonym:
  • product: acetyl-CoA synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS14380 [old locus tag: NWMN_2506 ]
  • symbol: NWMN_RS14380
  • product: acetyl-CoA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 2752404..2754002
  • length: 1599
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2752404..2754002) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    1021
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    1561
    ATGCAAAAGTCAGCTCTATTCGCGCCTGAAAAATATAACATTATTTCGGAAATCGAAAAA
    TATAGCCACATACCAGATAAAAAGGCAATTTTATATCATAATACTGAAGGCGAAGATATA
    TCAGTCACTTACCAACAGCTCATTGAACAGTCTAATAAAGTAGGTAATGTTTTAGCATCG
    CACGGTTTATCTAAAGGCGATAAAGTACTCATTATGATGCCACGATCTATTGCGACGTAT
    GAATTATATATTGCTGCTTTAAAATTAGGTGTTGCAATCATTCCATGTTCTGAAATGCTA
    AGAACTAAAGATTTACAATACCGTATCACACATGGTGAAATTAATGCAGTTATTGCACTA
    GAGCCATTCACAGTTGAATTTGAAAAAATTAAAGAATATGATGCGTTAACTAAATTTGTT
    ATTGCCGGTCAAAAAGATGGATGGATTTCACTTGAAGCTGAAAAAGAAAACGCAAGTAAC
    AAACTAGAAATGGCAGATACGACGCGTGATGATATGGCGATTCTGTCATATACATCTGGT
    ACAACTGGCAATCCTAAAGCCGTTACACATTGTCATGGATGGGGATTTGCACATTTACAA
    ATGGCACCAAAACATTGGTTATGTATTAATGAAGATGACTTAGTTTGGGCAACAGCAGCA
    CCAGGTTGGCAAAAATGGGTATGGAGTCCTTTCTTATCTGTATTAGGTTCAGGTGCAACT
    GCTTTTGTATTTAATGGAAGGTTCAGTCCTGAAACATACTTAGAATTGCTTCAAAAATAT
    CAAATTAATGTTTTATGTTGTACACCGACAGAATATCGTATGATGGCTAAATTACAGAAC
    TTAAATGATTATGACTTAACGCATTTACATAGTGCCGTTTCTGCAGGTGAGCCACTAAAT
    CGAGAAGTAGTTGAACAATTCAAGAAATACTTTAATTTAACTGTAAGAGATGGATATGGC
    CAGACTGAAAGTACCTTGCTTATAGGCTTTTTAAAAGATACACCACAGAGGATTGGATCA
    ATGGGTAAAGGCATTCCGGGTAGTTCGGTCACTGTAGTAGATGATGAGGGGAATAGCGTA
    CCTGCTAATACGAAAGGGAATATTGCAGTACCACTTGATTTGCCAGCACTTTTCAAAGGG
    TATTATAAAGAGCCAGAACGTACGGCGAAAGCGCAAACAGGTAAATACTACATCACTGGT
    GACTTAGCTCATGTAGATGATGATGGTTATTTCTGGTTTGAAGGACGTCGAGATGACATT
    ATTATTAGTTCAGGCTATACGATTGGGCCTTTTGAAGTTGAAGATGCGTTAACGAATCAT
    CCAGCCGTTAAAGAATGTGCTGTAGTAGCAAAACCTCATGATATACGAGGAAATATTGTT
    AAAGCATTCGTTATTTTACAGGACCATACTGCGGGTGATGATACGTTAGTTAAAGAACTA
    CAACAATTTGTTAAAAATGAAGTTGCACCATATAAATATCCGAGAGAGATTGAATTCGTT
    GACGACTTACCAAAAACAAACTCAGGTAAAATTCGTCGTGTTGAACTACGAGATGCTGAA
    ATAGAAAAATGGCAACAACAAAAAGATAGCAATCAATAA
    60
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    1560
    1599

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS14380 [old locus tag: NWMN_2506 ]
  • symbol: NWMN_RS14380
  • description: acetyl-CoA synthetase
  • length: 532
  • theoretical pI: 5.50431
  • theoretical MW: 59748.4
  • GRAVY: -0.349436

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    benzoate-CoA ligase family (TIGR02262; HMM-score: 295.6)
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 288)
    amino acid adenylation domain (TIGR01733; HMM-score: 185)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneO-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 180.8)
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 176.7)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 176.4)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 175.7)
    MetabolismTransport and binding proteinsCations and iron carrying compounds(2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 167.8)
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 160.9)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanD-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 145.9)
    MetabolismCentral intermediary metabolismOtheracetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 108.9)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 49.4)
    L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 33.3)
    MetabolismEnergy metabolismOtherphenylacetate-CoA ligase (TIGR02155; EC 6.2.1.30; HMM-score: 19)
    TIGR03089 family protein (TIGR03089; HMM-score: 12.9)
  • TheSEED:
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 280.7)
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 89.2)
    no clan definedAtu4866; Agrobacterium tumefaciens protein Atu4866 (PF11512; HMM-score: 13.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_RS0649050S ribosomal protein L19  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.113
    • Ymax_pos: 60
    • Cmax: 0.125
    • Cmax_pos: 60
    • Smax: 0.152
    • Smax_pos: 1
    • Smean: 0.093
    • D: 0.105
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447095084 NCBI
  • RefSeq: WP_001172340 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAEIEKWQQQKDSNQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS14380 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]