From AureoWiki
Jump to: navigation, search
NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS14720 [old locus tag: NWMN_2565 ]
  • pan locus tag?: SAUPAN006411000
  • symbol: NWMN_RS14720
  • pan gene symbol?: icaA
  • synonym:
  • product: poly-beta-1,6 N-acetyl-D-glucosamine synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS14720 [old locus tag: NWMN_2565 ]
  • symbol: NWMN_RS14720
  • product: poly-beta-1,6 N-acetyl-D-glucosamine synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 2832767..2834005
  • length: 1239
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2832767..2834005) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    TTGCAATTTTTTAACTTTTTGCTTTTTTATCCTGTATTTATGTCTATTTACTGGATTGTC
    GGTTCAATTTATTTCTATTTTACCAGAGAAATTAGATATTCATTGAACAAGAAGCCTGAC
    ATAAATGTGGATGAATTAGAAGGCATTACATTTTTACTTGCCTGTTATAACGAAAGTGAA
    ACGATTGAAGATACGTTGTCTAATGTTCTTGCACTCAAATACGAGAAGAAAGAAATTATT
    ATCATTAATGATGGAAGTTCAGATAATACAGCAGAACTCATCTATAAAATCAAAGAAAAT
    AATGACTTTATTTTCGTCGATTTACAAGAAAACAGAGGTAAAGCCAACGCACTCAATCAA
    GGCATTAAACAGGCTTCATATGATTATGTAATGTGCTTGGATGCAGATACTATCGTTGAT
    CAAGATGCACCATATTATATGATTGAGAATTTCAAACATGATCCAAAACTTGGTGCAGTT
    ACAGGTAATCCTAGAATTCGAAATAAGAGTTCTATTTTAGGTAAAATTCAAACGATAGAA
    TATGCAAGTTTAATTGGCTGTATTAAGCGAAGTCAGACACTTGCTGGCGCAGTCAATACT
    ATTTCGGGTGTCTTCACTCTATTTAAAAAAAGTGCAGTTGTCGACGTTGGCTACTGGGAT
    ACTGATATGATTACCGAAGATATTGCAGTTTCTTGGAAATTGCATTTACGTGGATATCGT
    ATTAAGTATGAACCGCTTGCCATGTGTTGGATGTTGGTTCCAGAAACATTGGGAGGTCTT
    TGGAAGCAACGCGTGAGATGGGCTCAAGGGGGACACGAAGTATTACTACGAGACTTTTTT
    AGCACAATGAAAACGAAAAGGTTTCCTTTATATATTTTGATGTTTGAGCAAATCATCTCG
    ATTTTATGGGTATATATAGTGCTTCTATATTTAGGCTATTTGTTCATAACAGCAAACTTC
    TTAGACTATACATTTATGACATATAGTTTTTCAATATTTCTACTATCATCATTTACTATG
    ACTTTTATAAACGTTATTCAATTTACAGTCGCACTCTTTATTGATAGTCGCTACGAGAAA
    AAGAATATGGCTGGACTCATATTTGTAAGTTGGTATCCGACAGTATACTGGATTATTAAC
    GCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCA
    ACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1239

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS14720 [old locus tag: NWMN_2565 ]
  • symbol: NWMN_RS14720
  • description: poly-beta-1,6 N-acetyl-D-glucosamine synthase
  • length: 412
  • theoretical pI: 8.45984
  • theoretical MW: 47769.2
  • GRAVY: 0.112864

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 633.2)
    putative glycosyltransferase, exosortase G-associated (TIGR03111; HMM-score: 98.7)
    chitooligosaccharide synthase NodC (TIGR04242; EC 2.4.1.-; HMM-score: 85.8)
    mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 57.5)
    cellulose synthase catalytic subunit (UDP-forming) (TIGR03030; EC 2.4.1.12; HMM-score: 50.2)
    Unknown functionEnzymes of unknown specificitytransferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 49.7)
    glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 32.7)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesrhamnosyltransferase (TIGR01556; EC 2.4.1.-; HMM-score: 28.8)
    hopene-associated glycosyltransferase HpnB (TIGR03469; HMM-score: 28)
    colanic acid biosynthesis glycosyltransferase WcaE (TIGR04009; EC 2.4.-.-; HMM-score: 22.6)
    hopanoid biosynthesis associated glycosyl transferase protein HpnI (TIGR03472; HMM-score: 20.4)
    colanic acid biosynthesis glycosyltransferase WcaA (TIGR04017; EC 2.4.-.-; HMM-score: 17.7)
  • TheSEED:
  • PFAM:
    GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 105.1)
    Glyco_trans_2_3; Glycosyl transferase family group 2 (PF13632; HMM-score: 93.6)
    Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 92.3)
    Glyco_transf_21; Glycosyl transferase family 21 (PF13506; HMM-score: 68.6)
    Chitin_synth_2; Chitin synthase (PF03142; HMM-score: 49.6)
    Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 39.4)
    Glyco_tranf_2_4; Glycosyl transferase family 2 (PF13704; HMM-score: 28)
    Glyco_tranf_2_5; Glycosyltransferase like family (PF13712; HMM-score: 24.2)
    Cellulose_synt; Cellulose synthase (PF03552; HMM-score: 22.5)
    AMP_N-like (CL0356) Creatinase_N_2; Creatinase/Prolidase N-terminal domain (PF16189; HMM-score: 17.9)
    no clan definedDUF393; Protein of unknown function, DUF393 (PF04134; HMM-score: 15.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.096
    • Ymax_pos: 11
    • Cmax: 0.113
    • Cmax_pos: 27
    • Smax: 0.113
    • Smax_pos: 1
    • Smean: 0.089
    • D: 0.093
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit source | edit]

  • GI: 447082174 NCBI
  • RefSeq: WP_001159430 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAVTGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYATWSSPDRGNTQR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS14720 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]