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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0008 [new locus tag: SA_RS00180 ]
  • pan locus tag?: SAUPAN000014000
  • symbol: hutH
  • pan gene symbol?: hutH
  • synonym:
  • product: histidine ammonia-lyase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0008 [new locus tag: SA_RS00180 ]
  • symbol: hutH
  • product: histidine ammonia-lyase
  • replicon: chromosome
  • strand: +
  • coordinates: 10900..12414
  • length: 1515
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGACTTTATATTTAGATGGTGAAACACTAACAATTGAGGATATTAAATCATTTTTACAA
    CAACAATCAAAGATTGAAATTATTGATGATGCGTTAGAACGTGTCAAAAAAAGTAGAGCG
    GTAGTTGAACGTATTATTGAAAATGAGGAAACGGTTTACGGTATCACTACAGGTTTTGGG
    TTATTTAGTGATGTACGTATAGACCCGACGCAATATAATGAATTACAAGTGAATCTGATA
    CGCTCACATGCCTGTGGACTAGGTGAGCCATTTTCAAAAGAAGTAGCATTAGTCATGATG
    ATTTTACGATTGAATACATTATTAAAAGGTCATTCAGGTGCCACTTTAGAATTAGTGAGA
    CAATTACAATTTTTTATAAATGAACGTATTATACCGATAATCCCACAACAAGGCTCTCTC
    GGTGCATCAGGAGATTTAGCGCCATTATCACATTTAGCATTAGCATTAATTGGTGAAGGG
    AAAGTATTGTACAGAGGGGAAGAAAAGGATAGTGACGATGTATTAAGAGAATTAAATAGA
    CAACCTTTGAACCTTCAGGCTAAAGAGGGTTTAGCATTGATTAATGGTACGCAAGCTATG
    ACAGCTCAAGGTGTCATTAGTTATATAGAAGCAGAAGATTTAGGTTACCAATCTGAATGG
    ATTGCTGCATTAACGCATCAGTCTCTTAATGGCATTATAGATGCATATCGACATGATGTG
    CACTCTGTTCGTAATTTTCAAGAACAGATTAATGTGGCAGCGCGTATGCGTGATTGGTTA
    GAAGGATCAACATTAACGACGCGACAAGCAGAAATACGTGTACAAGATGCATATACGTTG
    CGTTGTATACCACAAATCCATGGCGCGAGTTTTCAAGTATTCAATTATGTTAAACAGCAA
    TTAGAATTTGAAATGAATGCGGCTAATGATAATCCACTTATATTTGAGGAAGCAAATGAA
    ACGTTTGTTATTTCAGGTGGTAACTTCCATGGACAACCTATTGCTTTTGCATTAGATCAT
    CTTAAATTAGGTGTAAGTGAATTAGCAAACGTATCGGAACGTCGTCTAGAGCGACTAGTA
    AATCCTCAATTAAATGGTGATTTACCAGCATTTCTTAGTCCAGAGCCAGGATTGCAAAGT
    GGCGCGATGATTATGCAATATGCTGCTGCAAGTCTCGTTTCTGAAAATAAAACTTTAGCG
    CATCCAGCGAGTGTTGATTCTATCACTTCATCTGCGAACCAAGAAGATCACGTATCTATG
    GGAACTACAGCTGCTAGACATGGTTATCAAATTATTGAAAATGCAAGACGTGTGTTGGCA
    ATCGAATGTGTTATTGCATTACAAGCAGCAGAGTTGAAAGGTGTCGAAGGATTATCACCA
    AAAACACGTCGTAAATATGAGGAGTTTCGAAGTATCGTGCCATCCATTACACATGACCGT
    CAATTTCATAAAGATATTGAAGCGGTTGCACAGTATTTAAAGCAATCAATTTATCAAACG
    ACTGCATGTCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1515

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0008 [new locus tag: SA_RS00180 ]
  • symbol: HutH
  • description: histidine ammonia-lyase
  • length: 504
  • theoretical pI: 5.14928
  • theoretical MW: 56089.2
  • GRAVY: -0.185714

Function[edit source | edit]

  • reaction:
    EC 4.3.1.3?  ExPASy
    Histidine ammonia-lyaseL-histidine = urocanate + NH3
  • TIGRFAM:
    MetabolismEnergy metabolismAmino acids and amineshistidine ammonia-lyase (TIGR01225; EC 4.3.1.3; HMM-score: 664.2)
    tyrosine 2,3-aminomutase (TIGR03832; EC 5.4.3.6; HMM-score: 443.7)
    phenylalanine aminomutase (D-beta-phenylalanine forming) (TIGR04475; EC 5.4.3.11; HMM-score: 397.4)
    phenylalanine ammonia-lyase (TIGR01226; EC 4.3.1.24; HMM-score: 269.3)
    phenylalanine aminomutase (L-beta-phenylalanine forming) (TIGR04473; EC 5.4.3.10; HMM-score: 144.5)
  • TheSEED:  
    Amino Acids and DerivativesHistidine MetabolismHistidine Degradation Histidine ammonia-lyase (EC 4.3.1.3) 
  • PFAM:
    no clan definedLyase_aromatic; Aromatic amino acid lyase (PF00221; HMM-score: 611.5)
    UPF0223; Uncharacterised protein family (UPF0223) (PF05256; HMM-score: 14.5)
    RNR_N; Ribonucleotide reductase N-terminal (PF08343; HMM-score: 12.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.097
    • Ymax_pos: 26
    • Cmax: 0.115
    • Cmax_pos: 26
    • Smax: 0.107
    • Smax_pos: 33
    • Smean: 0.071
    • D: 0.087
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVERIIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMILRLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEGKVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQSEWIAALTHQSLNGIIDAYRHDVHSVRNFQEQINVAARMRDWLEGSTLTTRQAEIRVQDAYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPIAFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASLVSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAAELKGVEGLSPKTRRKYEEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]