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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0085 [new locus tag: SA_RS00585 ]
  • pan locus tag?: SAUPAN000382000
  • symbol: SA0085
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0085 [new locus tag: SA_RS00585 ]
  • symbol: SA0085
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 95829..96815
  • length: 987
  • essential: yes DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAGAAAATTTTTGGAGTGAATTACCACGTCCATTTTTTATTTTGGCGCCAATGGAA
    GACGTTACAGATATCGTCTTTCGACACGTTGTAAGTGAAGCAGCTAGACCGGATGTGTTT
    TTCACTGAATTTACAAATACTGAAAGCTTTTGCCACCCTGAAGGCATACATAGTGTGCGC
    GGACGCTTAACTTTTAGCGAAGATGAACACCCGATGGTCGCTCATATATGGGGAGATAAG
    CCAGAACAGTTCCGTGAAACGAGTATTCAACTAGCTAAAATGGGCTTTAAAGGCATTGAC
    TTAAATATGGGATGTCCTGTAGCAAATGTTGCTAAAAAGGGTAAGGGTTCCGGCTTAATC
    TTAAGACCGGATGTCGCTGCCGAAATTATTCAAGCAACTAAAGCAGGTGGGCTTCCGGTA
    AGTGTTAAAACACGCCTTGGCTACTATGAAATCGATGAATGGAAAGATTGGTTGAAGCAC
    GTCTTCGAACAAGATATTGCCAATTTATCTATTCATCTTCGTACACGTAAAGAAATGAGT
    AAAGTAGATGCACATTGGGAATTAATCGAAGCTATTAAAAATTTACGTGACGAAATTGCA
    CCAAATACATTGTTAACAATTAACGGTGATATTCCCGATAGAAAAACAGGACTTGAACTG
    GCAGAAAAATATGGCATCGATGGTGTCATGATTGGTAGAGGCATTTTCCATAATCCATTC
    GCTTTTGAAAAAGAACCACGCGAACACACAAGCAAGGAACTATTAGATCTATTGAGATTG
    CATTTATCATTGTTTAACAAATATGAAAAAGATGAAATACGACAATTCAAGAGCTTGCGT
    AGATTCTTTAAAATCTATGTGCGTGGCATAAGAGGCGCTAGCGAACTTCGCCATCAATTG
    ATGAACACACAATCAATTGCAGAAGCACGAGCACTTCTAGATGAATTTGAAGCCCAAATG
    GACGAAGACGTTAAAATAGAATTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    987

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0085 [new locus tag: SA_RS00585 ]
  • symbol: SA0085
  • description: hypothetical protein
  • length: 328
  • theoretical pI: 6.24812
  • theoretical MW: 37776.1
  • GRAVY: -0.374695

Function[edit source | edit]

  • reaction:
    EC 1.3.1.-?  ExPASy
    5,6-dihydrouracil in tRNA + NAD(P)+ = uracil in tRNA + NAD(P)H?
  • TIGRFAM:
    Unknown functionGeneralputative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 161.6)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA dihydrouridine synthase A (TIGR00742; EC 1.-.-.-; HMM-score: 91.8)
    dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 19.1)
    Unknown functionGeneralputative TIM-barrel protein (TIGR00736; HMM-score: 15.4)
  • TheSEED:  
    tRNA modification Archaea tRNA-dihydrouridine synthase 2 
  • PFAM:
    TIM_barrel (CL0036) Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 169.4)
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 15.9)
    TIM_barrel (CL0036) AP_endonuc_2; Xylose isomerase-like TIM barrel (PF01261; HMM-score: 13.5)
    no clan definedMPS2; Monopolar spindle protein 2 (PF17060; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: FMN
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.119
    • Ymax_pos: 35
    • Cmax: 0.135
    • Cmax_pos: 35
    • Smax: 0.15
    • Smax_pos: 2
    • Smean: 0.086
    • D: 0.106
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVRGRLTFSEDEHPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA0085 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]