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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0160 [new locus tag: SA_RS00970 ]
  • pan locus tag?: SAUPAN001001000
  • symbol: SA0160
  • pan gene symbol?: isdI
  • synonym:
  • product: heme-degrading monooxygenase IsdI

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0160 [new locus tag: SA_RS00970 ]
  • symbol: SA0160
  • product: heme-degrading monooxygenase IsdI
  • replicon: chromosome
  • strand: -
  • coordinates: 184042..184368
  • length: 327
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    ATGTTTATGGCAGAAAATAGATTACAATTACAAAAAGGCAGTGCGGAAGAAACGATTGAA
    CGTTTTTACAATAGACAAGGCATTGAAACTATTGAAGGCTTCCAACAAATGTTTGTCACT
    AAAACATTAAATACCGAGGATACAGACGAAGTTAAAATCTTAACTATTTGGGAATCTGAA
    GATAGCTTTAATAATTGGTTGAATTCCGATGTATTTAAAGAAGCTCATAAAAATGTACGT
    TTAAAAAGTGATGACGATGGACAGCAAAGTCCAATATTATCAAATAAAGTATTCAAATAT
    GATATTGGCTACCACTATCAAAAATAA
    60
    120
    180
    240
    300
    327

Protein[edit source | edit]

Protein Data Bank: 2ZDP
Protein Data Bank: 3LGM
Protein Data Bank: 3LGN
Protein Data Bank: 3QGP
Protein Data Bank: 4FNH
Protein Data Bank: 4FNI

General[edit source | edit]

  • locus tag: SA0160 [new locus tag: SA_RS00970 ]
  • symbol: SA0160
  • description: heme-degrading monooxygenase IsdI
  • length: 108
  • theoretical pI: 4.60532
  • theoretical MW: 12791.1
  • GRAVY: -0.863889

Function[edit source | edit]

  • reaction:
    EC 1.14.99.48?  ExPASy
    Heme oxygenase (staphylobilin-producing)Protoheme + 4 AH2 + 4 O2 = 5-oxo-delta-bilirubin + Fe2+ + CO + 4 A + 4 H2OProtoheme + 4 AH2 + 4 O2 = 15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O
    EC 1.14.99.3?  ExPASy
    Transferred entry: 1.14.14.18
  • TIGRFAM:
  • TheSEED:  
    Heme, hemin uptake and utilization systems in GramPositives Heme-degrading monooxygenase, staphylobilin-producing (EC 1.14.99.48) 
  • PFAM:
    Dim_A_B_barrel (CL0032) ABM; Antibiotic biosynthesis monooxygenase (PF03992; HMM-score: 60)
    Dehydratase_hem; Haem-containing dehydratase (PF13816; HMM-score: 13.6)
    DUF4937; Domain of unknown function (DUF4937 (PF16291; HMM-score: 13.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.142
    • Ymax_pos: 16
    • Cmax: 0.135
    • Cmax_pos: 16
    • Smax: 0.194
    • Smax_pos: 15
    • Smean: 0.145
    • D: 0.143
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLNTEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDIGYHYQK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Woo Cheol Lee, Michelle L Reniere, Eric P Skaar, Michael E P Murphy
Ruffling of metalloporphyrins bound to IsdG and IsdI, two heme-degrading enzymes in Staphylococcus aureus.
J. Biol. Chem.: 2008, 283(45);30957-63
[PubMed:18713745] [WorldCat.org] [DOI] (P p)
Michelle L Reniere, Georgia N Ukpabi, S Reese Harry, Donald F Stec, Robert Krull, David W Wright, Brian O Bachmann, Michael E Murphy, Eric P Skaar
The IsdG-family of haem oxygenases degrades haem to a novel chromophore.
Mol. Microbiol.: 2010, 75(6);1529-38
[PubMed:20180905] [WorldCat.org] [DOI] (I p)