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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0228 [new locus tag: SA_RS01335 ]
  • pan locus tag?: SAUPAN001098000
  • symbol: SA0228
  • pan gene symbol?: prsS
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0228 [new locus tag: SA_RS01335 ]
  • symbol: SA0228
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 275772..276914
  • length: 1143
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTCATCAGCAAGTACACAATCAACTAAGACGTCCGACATACATAATGAATCTATCAAT
    AAACAAATGGAAGCCAAAGCGCATGAAACAGCGCAAAATGCAGATTTAAAAACCGAAGCA
    AGAAGTTTATTTGATAATGCAACCAAATCAATCGGTAGACTAGCCGGCAATGATGAAAGC
    TTAAATCTTAATTTAAAAGATATGTTTTCTGAAGTATTTAAGCCGCATACTAAAAACGAA
    GCAGATGAAATATTTATAGCGGGTACTGCTAAAACTACGCCAGCAATTTGTGACATATCA
    GAAGAATGGGGGAAGCCATGGCTCTTTTCTCGAGTATTCATCGCTTTCACAGTAACATTT
    ATTGGATTATGGGTCATGGCAGCGATTTTTAATAACACTAACGCGATTCCGGGTCTCATT
    TTTATAGGGGCTTTAACAGTACCATTATCGGGTTTGTTCTTCTTTTATGAATCAAATGCG
    TTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGGCGGCGTATTTTCA
    TTACTAAGTACGATGGTATTATATAGATTTGTCGTTTTTAGTGATCAATTCGAAAGGTTT
    GGTTCTTTAACATTTTTCGATGCATTTTTAGTAGGATTAGTTGAAGAAACTGGAAAAGCA
    CTCATTATTGTTTATTTCGTCAATAAATTGAAAACAAATAAGATTTTGAATGGATTATTA
    ATCGGTGCTGCTATTGGTGCAGGGTTCGCAGTTTTTGAATCAGCAGGTTATATTTTGAAT
    TTCGCTTTAGGGGAAAATGTCCCATTATTAGATATTGTCTTCACACGTGCGTGGACTGCG
    ATTGGTGGTCATTTAGTTTGGTCAGCGATTGTTGGTGCTGCAATAGTTATTGCGAAAGAA
    CAGCATGGCTTTGAATTCAAAGATATTTTTGATAAACGCTTTTTAATATTCTTTTTATCA
    GCCGTTGGTTTACATGGCATTTGGGATACATCTTTAACTGTACTTGGCAGTGATACGTTG
    AAAATATTTATTTTAATCGTTATTGTGTGGATACTTGTATTCATTTTAATGGGGGCAGGT
    TTAAAACAAGTGAATTTACTGCAGAAAGAATTTAAAGAACAACAGAAAAAAGTAGACGAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1143

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0228 [new locus tag: SA_RS01335 ]
  • symbol: SA0228
  • description: hypothetical protein
  • length: 380
  • theoretical pI: 5.68735
  • theoretical MW: 42130.7
  • GRAVY: 0.482105

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    FIG01108038: hypothetical protein 
  • PFAM:
    Peptidase_U (CL0472) PrsW-protease; Protease prsW family (PF13367; HMM-score: 92.4)
    Sm-like (CL0527) LSM; LSM domain (PF01423; HMM-score: 13.4)
    no clan definedMWFE; NADH-ubiquinone oxidoreductase MWFE subunit (PF15879; HMM-score: 9.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.206
    • Ymax_pos: 28
    • Cmax: 0.2
    • Cmax_pos: 28
    • Smax: 0.28
    • Smax_pos: 1
    • Smean: 0.185
    • D: 0.198
  • predicted transmembrane helices (TMHMM): 8

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSSASTQSTKTSDIHNESINKQMEAKAHETAQNADLKTEARSLFDNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEEWGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNAFKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETGKALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTRAWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLTVLGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA0228 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Christina N Krute, Harris Bell-Temin, Halie K Miller, Frances E Rivera, Andy Weiss, Stanley M Stevens, Lindsey N Shaw
The membrane protein PrsS mimics σS in protecting Staphylococcus aureus against cell wall-targeting antibiotics and DNA-damaging agents.
Microbiology (Reading, Engl.): 2015, 161(Pt 5);1136-48
[PubMed:25741016] [WorldCat.org] [DOI] (I p)