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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0309 [new locus tag: SA_RS01780 ]
  • pan locus tag?: SAUPAN001245000
  • symbol: geh
  • pan gene symbol?: geh
  • synonym:
  • product: glycerol ester hydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0309 [new locus tag: SA_RS01780 ]
  • symbol: geh
  • product: glycerol ester hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 365458..367533
  • length: 2076
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTAAGAGGACAAGAAGAAAGAAAGTATAGTATTAGAAAGTATTCAATAGGCGTGGTG
    TCAGTGTTAGCGGCTACAATGTTTGTTGTGTCATCACATGAAGCACAAGCCTCGGAAAAA
    ACACCAACGTCCAATGCAGCGGCACAAAAAGAAACACTAAATCAACCGGGAGAACAAGGG
    AACGCGATAACGTCACATCAAATGCAGTCAGGAAAGCAATTAGACGATATGCATAAAGAG
    AATGGTAAAAGTGGAACAGTGACAGAAGGTAAAGACACGCTTCAATCATCGAAGCATCAA
    TCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCT
    GAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAACGTCAA
    AATGATCAGGTTCAAAATACCCATCATGCTGAACGTAATGGATCACAATCGACAACGTCA
    CAATCGAATGATGTTGATAAATCACAACCATCCATTCCGGCACAAAAGGTATTACCCAAT
    CATGATAAAGCAGCACCAACTTCAACTACACCCCCGTCTAATGATAAAACTGCACCTAAA
    TCAACAAAAGCACAAGATGCAACCACGGACAAACATCCAAATCAACAAGATACACATCAA
    CCCGCGCATCAAATCATAGATGCAAAGCAAGATGATACTGTTCGCCAAAGTGAACAGAAA
    CCACAAGTTGGCGATTTAAGTAAACATATCGATGGTCAAAATTCCCCAGAGAAACCGACA
    GATAAAAATACTGATAATAAACAACTAATCAAAGATGCGCTTCAAGCGCCTAAAACACGT
    TCGACTACAAATGCAGCAGCAGATGCTAAAAAGGTTCGACCACTTAAAGCGAATCAAGTA
    CAACCACTTAACAAATATCCAGTTGTTTTTGTACATGGATTTTTAGGATTAGTAGGCGAT
    AATGCACCTGCTTTATATCCAAATTATTGGGGTGGAAATAAATTTAAAGTTATCGAGGAA
    TTGAGAAAGCAAGGCTATAATGTACATCAAGCAAGTGTAAGTGCATTTGGTAGTAACTAT
    GATCGCGCTGTAGAACTTTATTATTACATTAAAGGTGGTCGCGTAGATTATGGCGCAGCA
    CATGCAGCTAAATACGGACATGAGCGCTATGGTAAGACTTATAAAGGAATCATGCCTAAT
    TGGGAACCTGGTAAAAAGGTACATCTTGTAGGGCATAGTATGGGTGGTCAAACAATTCGT
    TTAATGGAAGAGTTTTTAAGAAATGGTAACAAAGAAGAAATTGCCTATCATAAAGCGCAT
    GGTGGAGAAATATCACCATTATTCACTGGTGGTCATAACAATATGGTTGCATCAATCACA
    ACATTAGCAACACCACATAATGGTTCACAAGCAGCTGATAAGTTTGGAAATACAGAAGCT
    GTTAGAAAAATCATGTTCGCTTTAAATCGATTTATGGGTAACAAGTATTCGAATATCGAT
    TTAGGATTAACGCAATGGGGCTTTAAACAATTACCAAATGAGAGTTACATTGACTATATA
    AAACGCGTTAGTAAAAGCAAAATTTGGACATCAGACGATAATGCTGCCTATGATTTAACG
    TTAGATGGCTCTGCAAAATTGAACAACATGACAAGTATGAATCCTAATATTACGTATACG
    ACTTATACAGGTGTGTCTTCACATACTGGTCCATTAGGTTATGAAAATCCTGATTTAGGT
    ACATTTTTCTTAATGGATACAACGAGTAGAATTATTGGTCATGATGCAAGAGAAGAATGG
    CGTAAAAATGATGGTGTCGTACCAGTGATTTCGTCGTTACATCCATCCAATCAACCATTT
    ATTAATGTTACGAATGATGAACCTGCCACACGCAGAGGTATCTGGCAAGTTAAACCAATC
    ATACAAGGATGGGATCATGTCGATTTTATCGGTGTGGACTTCCTGGATTTCAAACGTAAA
    GGTGCAGAACTTGCCAACTTCTATACAGGTATTATAAATGACTTGTTGCGTGTGGAAGCG
    ACTGAAAGTAAAGGAACACAATTGAAAGCAAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2076

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0309 [new locus tag: SA_RS01780 ]
  • symbol: Geh
  • description: glycerol ester hydrolase
  • length: 691
  • theoretical pI: 9.48776
  • theoretical MW: 76542.3
  • GRAVY: -0.882634

Function[edit source | edit]

  • reaction:
    EC 3.1.1.3?  ExPASy
    Triacylglycerol lipaseTriacylglycerol + H2O = diacylglycerol + a carboxylate
  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 48.5)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsTriacylglycerolsTriacylglycerol metabolism Triacylglycerol lipase (EC 3.1.1.3) 
  • PFAM:
    no clan definedYSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 38.6)
    AB_hydrolase (CL0028) Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 21.6)
    LCAT; Lecithin:cholesterol acyltransferase (PF02450; HMM-score: 15.5)
    PGAP1; PGAP1-like protein (PF07819; HMM-score: 14.1)
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 7.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 3
    • Predicted Cleavage Site: HEAQASEK
  • SignalP: Signal peptide length 38 aa
    • Ymax: 0.705
    • Ymax_pos: 38
    • Cmax: 0.606
    • Cmax_pos: 38
    • Smax: 0.896
    • Smax_pos: 31
    • Smean: 0.671
    • D: 0.692
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTPTSNAAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQNSKTIRTQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQSNDVDKSQPSIPAQKVLPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTHQPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAPKTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKFKVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGGHNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFKQLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSSHTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFINVTNDEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATESKGTQLKAS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • geh no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]