NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0378 [new locus tag: SA_RS02160 ]
• pan locus tag^?: SAUPAN002056000
• symbol: SA0378
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Gene

⊟General

• type: CDS
• locus tag: SA0378 [new locus tag: SA_RS02160 ]
• symbol: SA0378
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 437087..437791
• length: 705
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123160 NCBI
• RefSeq: NP_373627 NCBI
• MicrobesOnline: 102653 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTCTAAAAATATCAATCAAGCAAATAGTAAATTAAATACGTCAAACAAAAAACTAAAA
  CAAGCTTATTCAAAATCCGATAGTAAAAACATGAAAGTTATATACATGCCTCATTGGCTA
  GAGTTTTATTCAATTGCTATACATAGCGATGTTACAAGCAATAAAAAAAGATATTATAAA
  TCATTTAGTAGAGGAACAGTGGTATATGTAAAACTAGGGAGTAATATTGGAAGTGAGTTT
  TCTGGTAATCATTTTTGTGTAATATTAGATAACAAAGACAATAAAGGAAAAGAAACAGTT
  ACAATAGTTCCACTTTCTTCCAAAGGTAATAAAAATTATTTGAAGTTAAACGAATCGGTG
  CTAAATCTAACAGCAACAGATTTAAAAAAACAGATTATCGATATCAGTAGTAAAGTACAA
  GCATTAACCCAAAAATATAGAGATATTGACATTAAACTTAGCACAGAAGATAAAAAGCTT
  TTGTCTAACTTAAATCAAAAAGCTAATGAACTGTATAGAGTGATAGGTGTTTATTCCAAG
  CACAAAGGGAAAGATACATACGTTAATATATCAGCAATTACAACAATAAGCAAAAGACGT
  ATAAGCAAAATAAATGATTCTGATCCTACTGGAACGATAATAATTTCAGAAGATGACATG
  AATGAAATCGAAAAGCAATTAAAGATAAAGTTCTTTTCTAATTGA
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⊟Protein

⊟General

• locus tag: SA0378 [new locus tag: SA_RS02160 ]
• symbol: SA0378
• description: hypothetical protein
• length: 234
• theoretical pI: 10.3041
• theoretical MW: 26618.3
• GRAVY: -0.616239

⊟Function

• reaction:
• TIGRFAM:
  MetabolismFatty acid and phospholipid metabolismBiosynthesisacetyl-CoA carboxylase, carboxyl transferase, alpha subunit (TIGR00513; EC 6.4.1.2; HMM-score: 12.9)
• TheSEED:  
  Pathogenicity island SaPIn2 
• PFAM:
  CcdB_PemK (CL0624) PemK_toxin; PemK-like, MazF-like toxin of type II toxin-antitoxin system (PF02452; HMM-score: 52)
  GCS (CL0286) Glu_cys_ligase; Glutamate-cysteine ligase (PF04262; HMM-score: 16.4)
  no clan definedMuted; Organelle biogenesis, Muted-like protein (PF14942; HMM-score: 11.1)
  Mt_ATP_synt; Mitochondrial ATP synthase subunit (PF15704; HMM-score: 10.4)
  DASH_Dad4; DASH complex subunit Dad4 (PF08650; HMM-score: 7.9)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.217
  • Ymax_pos: 23
  • Cmax: 0.133
  • Cmax_pos: 23
  • Smax: 0.492
  • Smax_pos: 7
  • Smean: 0.373
  • D: 0.278
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926094 NCBI
• RefSeq: NP_373627 NCBI
• UniProt: A0A0H3JSW7 UniProt

⊟Protein sequence

• MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYSIAIHSDVTSNKKRYYKSFSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTIVPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALTQKYRDIDIKLSTEDKKLLSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISEDDMNEIEKQLKIKFFSN

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0378 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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