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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0442 [new locus tag: SA_RS02550 ]
  • pan locus tag?: SAUPAN002219000
  • symbol: holB
  • pan gene symbol?: holB
  • synonym:
  • product: DNA polymerase III subunit delta'

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0442 [new locus tag: SA_RS02550 ]
  • symbol: holB
  • product: DNA polymerase III subunit delta'
  • replicon: chromosome
  • strand: +
  • coordinates: 514728..515654
  • length: 927
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATGAACAGCAACAATTGACGAATGCATATCATTCAAATAAATTATCGCATGCCTAT
    TTATTTGAAGGTGATGATGCACAAACGATGAAACAAGTTGCGATTAATTTTGCAAAGCTT
    ATTTTATGTCAAACAGATAGTCAATGTGAAACAAAGGTTAGTACATATAATCATCCAGAC
    TTTATGTATATATCAACAACTGAGAATGCAATTAAGAAAGAACAAGTTGAACAACTTGTG
    CGTCATATGAATCAACTTCCTATAGAAAGCACAAATAAAGTGTACATCATTGAAGACTTT
    GAAAAGTTAACTGTTCAAGGGGAAAACAGTATCTTGAAATTTCTTGAAGAACCACCGGAC
    AATACGATTGCTATTTTATTGTCTACAAAACCTGAGCAAATTTTAGACACAATCCATTCA
    AGGTGTCAGCATGTGTATTTCAAGCCTATTGATAAAGAAAAGTTTATAAATAGATTAGTT
    GAACAAAACATGTCTAAGCCAGTAGCTGAAATGATTAGTACTTATACTACGCAAATAGAT
    AATGCAATAGCTTTAAATGAAGAATTTGATTTATTAGCATTAAGGAAATCAGTTATACGT
    TGGTGTGAATTGTTGCTTACTAACAAGCCAATGGCACTTATAGGTATTATTGATTTATTG
    AAACAGGCTAAAAATAAAAAACTGCAATCTTTAACTATTGCAGCTGTGAATGGTTTCTTC
    GAAGATATCATACATACAAAGGTAAATGTAGAGGATAAACAAATATATAGTGATTTAAAA
    AATGATATTGATCAATATGCGCAAAAGTTGTCGTTTAATCAATTATTTTTGATGTTTGAT
    CAACTGACGGAAGCACATAAGAAATTGAATCAAAATGTAAATCCAACGCTTGTATTTGAA
    CAAATCGTAATTAAGGGTGTGAGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    927

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0442 [new locus tag: SA_RS02550 ]
  • symbol: HolB
  • description: DNA polymerase III subunit delta'
  • length: 308
  • theoretical pI: 5.41475
  • theoretical MW: 35531.6
  • GRAVY: -0.311364

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase III, delta' subunit (TIGR00678; EC 2.7.7.7; HMM-score: 153.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase III, subunit gamma and tau (TIGR02397; EC 2.7.7.7; HMM-score: 103.7)
    Cellular processesCellular processesSporulation and germinationATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 19.8)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 19.8)
    radical SAM/SPASM domain protein, ACGX system (TIGR04340; HMM-score: 15)
    Cellular processesCellular processesSporulation and germinationATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 13.8)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 13.8)
  • TheSEED:  
    DNA MetabolismDNA replicationDNA-replication DNA polymerase III delta prime subunit (EC 2.7.7.7) 
    Stress ResponseHeat shockHeat shock dnaK gene cluster extended DNA polymerase III delta prime subunit (EC 2.7.7.7) 
  • PFAM:
    P-loop_NTPase (CL0023) DNA_pol3_delta2; DNA polymerase III, delta subunit (PF13177; HMM-score: 155.9)
    post-AAA (CL0604) DNApol3-delta_C; DNA polymerase III, delta subunit, C terminal (PF09115; HMM-score: 23.9)
    DNA_pol3_gamma3; DNA polymerase III subunits gamma and tau domain III (PF12169; HMM-score: 19)
    no clan definedDUF1031; Protein of unknown function (DUF1031) (PF06275; HMM-score: 16.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.17
    • Ymax_pos: 50
    • Cmax: 0.151
    • Cmax_pos: 50
    • Smax: 0.279
    • Smax_pos: 45
    • Smean: 0.133
    • D: 0.155
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 15926161 NCBI
  • RefSeq: NP_373694 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQTDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEKLTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLVEQNMSKPVAEMISTYTTQIDNAIALNEEFDLLALRKSVIRWCELLLTNKPMALIGIIDLLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLFLMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]