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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0479 [new locus tag: SA_RS02820 ]
  • pan locus tag?: SAUPAN002286000
  • symbol: nupC
  • pan gene symbol?: nupC
  • synonym:
  • product: pyrimidine nucleoside transport protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0479 [new locus tag: SA_RS02820 ]
  • symbol: nupC
  • product: pyrimidine nucleoside transport protein
  • replicon: chromosome
  • strand: -
  • coordinates: 559257..560471
  • length: 1215
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCATATTCTAATTGGGATTATCGGAATTATCTTCTTTTTAGCACTTGCGTTTCTATTT
    AGCTCAGACAGGAAAAATATTCGCTGGAAATATGTAGGAATTTTACTTGCAATTCAGCTT
    ATCTTTGCATTTATCTTGCTTAAAACTACAATGGGAATCAAGGTTATTGGTGGTATTTCA
    AACGGTTTTAACTACTTATTATTAAAAGCAGCAGATGGTGTTAACTTTGTATTCGGTGGT
    ATTCAATATATCGATCCTAAGCAACCACCATTCTTCTTCAGTGTATTATTACCTATCGTA
    TTTATTTCAGCGATTATCGGTATCCTTCAATACACAAAGATTTTACCTTTAATCATTAAT
    GTTTTAGGATTCTTAATTTCTAAAATTAATGGTATGGGACGTTTAGAATCATACAATGCA
    GTTGCTGCAGCAATTTTAGGACAATCTGAAGTATTTATTTCAATTAAAAAACAATTACCA
    TACATACCTAAGCAACGTTTATACACATTAACAGCTTCAGCAATGTCGACTGTTTCTGCT
    TCAATTATCGGTGCATACTTTACCTTAATTGAACCTAAATATGTTGTAACGGCTGTTGTC
    TTAAACTTATTTGGTGGTTTCATCATCGCTTCAATTATTAACCCTTATAAAGTTAATGAA
    GAAGATGACAAATTATTAGTTGAAGAAACTGAAGAAAGACAACAATCATTCTTCGAAATG
    CTAGGAGAATATATTTTAGATGGATTTAAAGTAGCTGTCATTGTAGGTGCAATGTTAATC
    GGATACATTGCCATTATCGCTCTATTAAATGGAATTGTAAGCAATATCTTTAGTACAGTT
    TCAGGTGGCGCTATTTCTTGGGATTTCCAAACATTAATTGGTTTTGTCTTTGCACCATTT
    GCATTCTTAGTCGGTGTGCCATGGCAAGATGCAGTTCAAGCCGGTTCAGTTATGGCGACA
    AAACTATTATCTAACGAATTTGTAGCAATGCAAGCACTTGGTAAACTTAGTGATTTATCT
    GAACACGCAAAAGGTGTTACATCTGTATTCTTAGTATCATTTGCTAACTTTAGCTCTATT
    GGTATTATTTCAGGTGCTATTAAATCACTTAATGACAAAAAAGGAGATACAGTTGCCCGC
    TTTGGTTTGAAATTATTATTCGGTGCAACACTAGTATCTTTCATTTCAGCTGCAATCGCT
    GGATTCTTTATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1215

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0479 [new locus tag: SA_RS02820 ]
  • symbol: NupC
  • description: pyrimidine nucleoside transport protein
  • length: 404
  • theoretical pI: 9.44764
  • theoretical MW: 43804.7
  • GRAVY: 0.870792

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsNucleosides, purines and pyrimidinesnucleoside transporter, NupC family (TIGR00804; HMM-score: 433.4)
  • TheSEED:  
    CarbohydratesMonosaccharidesDeoxyribose and Deoxynucleoside Catabolism Nucleoside permease NupC 
    Nucleosides and NucleotidesPurinesXanthine Metabolism in Bacteria Nucleoside permease NupC 
  • PFAM:
    no clan definedNucleos_tra2_C; Na+ dependent nucleoside transporter C-terminus (PF07662; HMM-score: 210.9)
    Nucleos_tra2_N; Na+ dependent nucleoside transporter N-terminus (PF01773; HMM-score: 75.6)
    Gate; Nucleoside recognition (PF07670; HMM-score: 25)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.115
    • Ymax_pos: 11
    • Cmax: 0.148
    • Cmax_pos: 30
    • Smax: 0.17
    • Smax_pos: 3
    • Smean: 0.126
    • D: 0.12
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit source | edit]

  • GI: 15926198 NCBI
  • RefSeq: NP_373731 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAFILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFISAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLPYIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKVNEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNIFSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALGKLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLVSFISAAIAGFFI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • nupC no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]