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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0510 [new locus tag: SA_RS02980 ]
  • pan locus tag?: SAUPAN002324000
  • symbol: araB
  • pan gene symbol?: araB
  • synonym:
  • product: ribulokinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0510 [new locus tag: SA_RS02980 ]
  • symbol: araB
  • product: ribulokinase
  • replicon: chromosome
  • strand: +
  • coordinates: 596096..597733
  • length: 1638
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCGTATAGCATTGGAATTGATTATGGAACTGCTTCAGGCCGTGTGTTTTTAATTAAT
    ACAACTAACGGTCAAGTAGTATCAAAATTTGTGAAACCATATACACATGGTGTCATTGAA
    AGTGAATTAAATGGTTTGAAAATACCACATACATATGCACTTCAAAATAGTAATGATTAT
    TTAGAAATTATGGAAGAAGGAATATCATATATAGTACGTGAATCAAAAATAGATCCAGTC
    AATATAGTAGGTATTGGTATAGACTTTACTTCATCTACTATTATTTTTACTGACGAAAAC
    CTTAACCCGGTACATAATTTAAAACAATTTAAAAACAATCCACATGCGTATGTGAAACTT
    TGGAAACATCATGGTGCATATAAAGAAGCAGAGAAATTATATCAAACTGCTATTGAAAAT
    AATAATAAGTGGTTAGGCCATTATGGATATAATGTTAGTAGTGAATGGATGATTCCCAAA
    ATAATGGAAGTCATGAATCGAGCACCAGAAATTATGGAAAAAACGGCTTATATTATGGAA
    GCGGGCGATTGGATTGTAAATAAATTAACTAATAAAAATGTACGCTCGAATTGTGGATTA
    GGTTTCAAAGCATTTTGGGAAGAAGAAACAGGGTTTCATTATGATTTATTTGATAAAATA
    GACCCCAAATTATCAAAAGTAATTCAAGATAAAGTATCTGCACCGGTTGTTAATATTGGT
    GAAGTAGTAGGGAAACTGGACGATAAAATGGCACAGAAATTAGGATTATCAAAAGAGACT
    ATGGTAAGTCCTTTTATTATTGATGCCCATGCTAGTTTATTAGGTATTGGGTCTGAAAAA
    GATAAAGAAATGACTATGGTGATGGGAACAAGCACATGCCATCTTATGTTAAATGAAAAG
    CAACATCAAGTGCCAGGTATATCAGGTTCTGTAAAAGGAGCAATTATTCCAGAATTATTT
    GCTTATGAAGCGGGGCAATCAGCAGTAGGTGATTTGTTTGAGTATGTCGCTAAGCAAGCA
    CCAAAGTCATATGTAGATGAAGCAGCAAATAGAAATATGACTGTATTTGAATTAATGAAT
    GAAAAGATAAAACATCAAATGCCAGGTGAAAGTGGGCTCATTGCTCTTGATTGGCATAAT
    GGAAATCGAAGTGTATTAAGTGATAGCAATTTAACAGGTTGTATCTTTGGATTAACTTTA
    CAAACTAAGCATGAGGATATTTATAGAGCATATTTAGAAGCTACAGCATTTGGTACTAAG
    ATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGC
    GGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAA
    CTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTC
    AGTGGTGGCGCACATAATTCAATTAATGATGCAGTTGATGCTATGAAAGAGCCAATTTTA
    TACGAAATTAATCCAGAAGCGGAAAAAGTACAAAGGTATGAAACATTATTTAAAGCTTAT
    AAGGCTTTACATGATATCCATGGTTATAAAAAAGCTAATATAATGAAAGATATCCAGAGT
    TTAAGAGTTGAGGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1638

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0510 [new locus tag: SA_RS02980 ]
  • symbol: AraB
  • description: ribulokinase
  • length: 545
  • theoretical pI: 6.24004
  • theoretical MW: 60964.5
  • GRAVY: -0.249908

Function[edit source | edit]

  • reaction:
    EC 2.7.1.16?  ExPASy
    RibulokinaseATP + L(or D)-ribulose = ADP + L(or D)-ribulose 5-phosphate
  • TIGRFAM:
    MetabolismEnergy metabolismSugarsribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 554.4)
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 206.8)
    MetabolismEnergy metabolismSugarsxylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 153.8)
    MetabolismEnergy metabolismSugarsgluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 87.1)
    MetabolismEnergy metabolismOtherglycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 66.9)
    MetabolismEnergy metabolismSugarsL-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 59.5)
  • TheSEED:  
    Ribulokinase (EC 2.7.1.16) 
  • PFAM:
    Actin_ATPase (CL0108) FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 125.4)
    FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 63.3)
    no clan definedDUF4846; Domain of unknown function (4846) (PF16138; HMM-score: 17.5)
    Actin_ATPase (CL0108) BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.64
    • Cytoplasmic Membrane Score: 0.3
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.189
    • Ymax_pos: 30
    • Cmax: 0.17
    • Cmax_pos: 30
    • Smax: 0.367
    • Smax_pos: 28
    • Smean: 0.148
    • D: 0.173
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELNGLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPVNIVGIGIDFTSSTIIFTDENLNPVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPKIMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFDKIDPKLSKVIQDKVSAPVVNIGEVVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGIGSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFEYVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLTGCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMMDIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAEKVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]