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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0524 [new locus tag: SA_RS03050 ]
  • pan locus tag?: SAUPAN002343000
  • symbol: SA0524
  • pan gene symbol?: folE2
  • synonym:
  • product: GTP cyclohydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0524 [new locus tag: SA_RS03050 ]
  • symbol: SA0524
  • product: GTP cyclohydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 619909..620787
  • length: 879
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGACTGAATTTGATTTATCGACACGAGAAGGTCGTTGGAAACACTTTGGTTCCGTAGAT
    CCTATTGAAGGCACGAAACCTACTACTAAGAACGAAATGACAGATTTACAAAGTACACAT
    AAAGATTTTTTATTTGAAATAGAAGAAGTTGGTATTAAAAACTTAGTGTACCCTGTACTT
    GTTGACCAGTATCAAACAGCTGGCACATTTAGTTTTTCAACAAGTTTAACAAAAGACGAA
    AAAGGTATTAATATGAGCCGTATAATTGAAAGTGTTGAAAAGCACTATGATAATGGTATT
    GAACTTGAATTTAATACACTTTATCAGGTGTTACGCACTTTACAAACGAATATGAAACAA
    AATGCGGCAGGCGTTGATGTTTCTGGGAAATGGTTCTTTGACCGCTATAGTCCAACCACA
    AATATTAAAGCAGTTGGTAATGCCGATGTCACTTATGGATTAGCAATTGACGGTGATAAA
    GTTACACGTAAAGAACTTACTATTGAAGCAACTGTCACAACGCTATGCCCTTGTTCAAAA
    GAAATAAGTGAGTATTCAGCGCACAACCAACGTGGTGTTGTCACTGTTAAAACATATATT
    AATAAAGATCAAAACATAGTTGATGACTATAAAAACAAAATTTTAGATGCAATGGAAGCT
    AATGCAAGTTCAATTCTATACCCTATTTTAAAACGTCCTGATGAAAAACGTGTGACAGAG
    CGTGCATATGAAAATCCACGTTTTGTCGAAGACTTAATTCGTTTAATTGCCGCGGACTTA
    GTTGAATTTGATTGGTTAGACGGTTTCGATATCGAATGTCGTAATGAAGAGTCTATCCAT
    CAACATGATGCTTTCGCTAAATTAAAGTATCGCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    879

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0524 [new locus tag: SA_RS03050 ]
  • symbol: SA0524
  • description: GTP cyclohydrolase
  • length: 292
  • theoretical pI: 4.93644
  • theoretical MW: 33480.4
  • GRAVY: -0.542466

Function[edit source | edit]

  • reaction:
    EC 3.5.4.16?  ExPASy
    GTP cyclohydrolase IGTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherGTP cyclohydrolase (TIGR00294; EC 3.5.4.-; HMM-score: 169.1)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsFolate and pterinesFolate Biosynthesis GTP cyclohydrolase I (EC 3.5.4.16) type 2 
    RNA MetabolismRNA processing and modificationQueuosine-Archaeosine Biosynthesis GTP cyclohydrolase I (EC 3.5.4.16) type 2 
  • PFAM:
    THBO-biosyn (CL0334) GCHY-1; Type I GTP cyclohydrolase folE2 (PF02649; HMM-score: 235.2)
    HTH (CL0123) PadR; Transcriptional regulator PadR-like family (PF03551; HMM-score: 14.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.103
    • Ymax_pos: 42
    • Cmax: 0.104
    • Cmax_pos: 39
    • Smax: 0.123
    • Smax_pos: 1
    • Smean: 0.086
    • D: 0.096
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFLFEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIELEFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDKVTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQNIVDDYKNKILDAMEANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNEESIHQHDAFAKLKYRK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J. Microbiol. Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)