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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0656 [new locus tag: SA_RS03745 ]
  • pan locus tag?: SAUPAN002586000
  • symbol: nagA
  • pan gene symbol?: nagA
  • synonym:
  • product: N-acetylglucosamine-6-phosphate deacetylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0656 [new locus tag: SA_RS03745 ]
  • symbol: nagA
  • product: N-acetylglucosamine-6-phosphate deacetylase
  • replicon: chromosome
  • strand: +
  • coordinates: 750281..751462
  • length: 1182
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    GTGTCAGAATTAATTATATATAACGGCAAAGTTTATACTGAAGATGGCAAAATCGATAAT
    GGTTACATTCATGTGAAAGATGGACAGATTGTTGCAATTGGAGAAGGGGATGATAAAGCA
    GCAATTGATAATGATACGACAAATAAAATTCAAGTGATTGATGCTAAAGGTCATCATGTA
    TTACCAGGTTTTATTGATATACATATTCATGGTGGTTATGGTCAAGATGCAATGGATGGG
    TCATACGATGGCTTAAAATATCTATCCGAAAATTTGTTATCTGAAGGGACGACATCATAC
    TTGGCCACTACAATGACGCAATCTACTGATAAAATAGATAAAGCACTTACAAATATTGCT
    AAATATGAAGCGGAGCAAGATGTTCACAATGCAGCGGAAATTGTAGGTATACATTTAGAA
    GGACCATTTATATCTGAAAATAAAGTTGGTGCTCAACATCCGCAATACGTTGTACGCCCA
    TTTATCGATAAAATTAAACATTTTCAAGAGACTGCTAACAGATTAATAAAGATTATGACG
    TTTGCACCTGAAGTTGAAGGTGCAAAAGAAGCGCTTGAAACGTATAAAGATGACATTATT
    TTTTCAATTGGTCATACAGTGGCAACATACGAAGAAGCAGTCGAAGCTGTTGAGCGAGGA
    GCTAAACATGTCACGCATTTATATAATGCAGCGACGCCATTCCAACATAGAGAACCAGGT
    GTTTTTGGAGCAGCATGGTTGAATGATGCTCTACATACCGAAATGATTGTTGATGGCACA
    CATTCTCATCCGGCATCGGTTGCAATTGCTTACCGTATGAAAGGTAATGAACGTTTTTAT
    TTAATTACCGATGCAATGCGTGCAAAAGGTATGCCTGAAGGAGAATATGATTTGGGTGGA
    CAAAAAGTAACTGTTCAATCGCAACAAGCACGTCTTGCAAATGGTGCGCTTGCTGGTAGT
    ATTTTAAAAATGAATCATGGGTTACGTAACTTAATATCATTTACAGGTGATACATTAGAT
    CATTTATGGCGAGTAACAAGTTTAAATCAAGCCATTGCATTAGGTATCGATGATAGAAAA
    GGTAGTATTAAAGTAAATAAGGATGCAGATCTTGTTATTCTAGATGATGATATGAATGTA
    AAATCTACAATAAAACAAGGCAAGGTTCACACATTTAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0656 [new locus tag: SA_RS03745 ]
  • symbol: NagA
  • description: N-acetylglucosamine-6-phosphate deacetylase
  • length: 393
  • theoretical pI: 5.76193
  • theoretical MW: 43186.3
  • GRAVY: -0.325954

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismCentral intermediary metabolismAmino sugarsN-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 376.8)
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 45.9)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 44.3)
    MetabolismEnergy metabolismOtherphosphonate metabolism protein PhnM (TIGR02318; HMM-score: 41.5)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesOtherguanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 41.1)
    MetabolismEnergy metabolismAmino acids and aminesimidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 41)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisdihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 35.2)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesbeta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 35.1)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesSalvage of nucleosides and nucleotidesadenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 34.1)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 26.1)
    MetabolismCentral intermediary metabolismNitrogen metabolismurease, alpha subunit (TIGR01792; EC 3.5.1.5; HMM-score: 20.8)
    putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 18.6)
    MetabolismEnergy metabolismAmino acids and aminesformiminoglutamate deiminase (TIGR02022; EC 3.5.3.13; HMM-score: 15.4)
  • TheSEED:  
    CarbohydratesAminosugarsChitin and N-acetylglucosamine utilization N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25) 
    Cell Wall and CapsuleCapsular and extracellular polysacchridesSialic Acid Metabolism N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25) 
    Cell Wall and Capsule - no subcategoryUDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25) 
  • PFAM:
    Amidohydrolase (CL0034) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 95.9)
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 56.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.089
    • Ymax_pos: 22
    • Cmax: 0.106
    • Cmax_pos: 41
    • Smax: 0.108
    • Smax_pos: 17
    • Smean: 0.068
    • D: 0.081
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 15926378 NCBI
  • RefSeq: NP_373911 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEGDDKAAIDNDTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLATTMTQSTDKIDKALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRPFIDKIKHFQETANRLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVERGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGNERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISFTGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTFS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]