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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00710
  • pan locus tag?: SAUPAN002586000
  • symbol: SAOUHSC_00710
  • pan gene symbol?: nagA
  • synonym:
  • product: N-acetylglucosamine-6-phosphate deacetylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00710
  • symbol: SAOUHSC_00710
  • product: N-acetylglucosamine-6-phosphate deacetylase
  • replicon: chromosome
  • strand: +
  • coordinates: 694195..695376
  • length: 1182
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    GTGTCAGAATTAATTATATATAACGGCAAAGTTTATACTGAAGATGGCAAAATCGATAAT
    GGTTACATTCATGTGAAAGATGGACAGATTGTTGCAATTGGAGAAGTGGATGATAAAGCA
    GCAATTGATAATGATACGACAAATAAAATTCAAGTGATTGATGCTAAAGGTCATCATGTA
    TTACCAGGTTTTATTGATATACATATTCATGGTGGTTATGGTCAAGATGCAATGGATGGG
    TCATACGATGGCTTAAAATATCTATCCGAAAATTTGTTGTCTGAAGGGACGACATCATAC
    TTGGCCACTACAATGACGCAATCGACTGATAAAATAGATAATGCACTTACAAATATTGCT
    AAATATGAAGCGGAGCAAGATGTTCACAATGCAGCGGAAATTGTAGGTATACATTTAGAA
    GGACCATTTATATCTGAAAATAAAGTTGGTGCTCAACATCCGCAATACGTTGTACGCCCA
    TTTATCGATAAAATTAAACATTTTCAAGAGACTGCTAACGGATTAATAAAGATTATGACG
    TTTGCACCTGAAATTGAAGGTGCAAAAGAAGCGCTTGAAACGTATAAAGATGACATTATT
    TTTTCAATTGGTCATACAGTAGCAACATACGAAGAAGCAGTTGAAGCTGTTGAGCGAGGA
    GCTAAACATGTCACGCATTTATATAATGCAGCGACGCCATTCCAACATAGAGAACCAGGT
    GTTTTTGGAGCAGCATGGTTGAATGATGCTCTACATACCGAAATGATTGTTGATGGCACT
    CATTCTCATCCGGCATCGGTTGCAATTGCTTACCGTATGAAAGGTAATGAACGTTTTTAT
    TTAATTACCGATGCAATGCGTGCAAAAGGTATGCCTGAAGGAGAATATGATTTGGGTGGA
    CAAAAAGTAACTGTTCAATCGCAACAAGCACGTCTTGCAAATGGTGCGCTTGCTGGTAGT
    ATTTTAAAAATGAATCATGGGTTACGTAACTTAATATCATTTACAGGTGATACATTAGAT
    CATTTATGGCGAGTAACAAGTTTAAATCAAGCCATTGCATTAGGTATCGATGATAGAAAA
    GGTAGTATTAAAGTAAATAAGGATGCAGATCTTGTTATTCTAGATGATGATATGAATGTA
    AAATCTACAATAAAACAAGGCAAGGTTCACACATTTAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00710
  • symbol: SAOUHSC_00710
  • description: N-acetylglucosamine-6-phosphate deacetylase
  • length: 393
  • theoretical pI: 5.49488
  • theoretical MW: 43129.2
  • GRAVY: -0.302036

Function[edit source | edit]

  • reaction:
    EC 3.5.1.25?  ExPASy
    N-acetylglucosamine-6-phosphate deacetylaseN-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate
  • TIGRFAM:
    MetabolismCentral intermediary metabolismAmino sugarsN-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 378.6)
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 46.2)
    MetabolismEnergy metabolismAmino acids and aminesimidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 44.2)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 42.9)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesOtherguanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 41.1)
    MetabolismEnergy metabolismOtherphosphonate metabolism protein PhnM (TIGR02318; HMM-score: 39.6)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesbeta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 36.3)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesSalvage of nucleosides and nucleotidesadenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 34.2)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisdihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 34.1)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 26.4)
    MetabolismCentral intermediary metabolismNitrogen metabolismurease, alpha subunit (TIGR01792; EC 3.5.1.5; HMM-score: 19.7)
    putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 17.8)
  • TheSEED:  
    CarbohydratesAminosugarsChitin and N-acetylglucosamine utilization N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25) 
    Cell Wall and CapsuleCapsular and extracellular polysacchridesSialic Acid Metabolism N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25) 
    Cell Wall and Capsule - no subcategoryUDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25) 
  • PFAM:
    Amidohydrolase (CL0034) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 96.1)
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 56.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.088
    • Ymax_pos: 22
    • Cmax: 0.104
    • Cmax_pos: 42
    • Smax: 0.107
    • Smax_pos: 17
    • Smean: 0.067
    • D: 0.08
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDNDTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLATTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRPFIDKIKHFQETANGLIKIMTFAPEIEGAKEALETYKDDIIFSIGHTVATYEEAVEAVERGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGNERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISFTGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTFS

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]