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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0746 [new locus tag: SA_RS04245 ]
  • pan locus tag?: SAUPAN002800000
  • symbol: SA0746
  • pan gene symbol?: nuc
  • synonym:
  • product: nuclease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0746 [new locus tag: SA_RS04245 ]
  • symbol: SA0746
  • product: nuclease
  • replicon: chromosome
  • strand: +
  • coordinates: 855748..856434
  • length: 687
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACAGAATACTTATTAAGTGCTGGCATATGTATGGCAATTGTTTCAATATTACTTATA
    GGGATGGCTATCAGTAATGTTTCGAAAGGGCAATACGCAAAGAGGTTTTTCTTTTTCGCT
    ACTAGTTGCTTAGTGTTAACTTTAGTTGTAGTTTCAAGTCTAAGTAGCTCAGCAAATGCA
    TCACAAACAGATAACGGCGTAAATAGAAGTGGTTCTGAAGATCCAACAGTATATAGTGCA
    ACTTCAACTAAAAAATTACATAAAGAACCTGCGACATTAATTAAAGCGATTGATGGTGAT
    ACGGTTAAATTAATGTACAAAGGTCAACCAATGACATTCAGACTATTATTAGTTGATACA
    CCTGAAACAAAGCATCCTAAAAAAGGTGTAGAGAAATATGGCCCTGAAGCAAGTGCATTT
    ACGAAAAAAATGGTAGAAAATGCAAATAAAATTGAAGTCGAGTTTGACAAAGGTCAAAGA
    ACTGATAAATATGGACGTGGCTTAGCGTATATTTATGCTGATGGAAAAATGGTAAACGAA
    GCTTTAGTTCGTCAAGGCTTGGCTAAAGTTGCTTATGTTTATAAACCTAACAATACACAT
    GAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGC
    GAAGACAACGCTGATTCAGGTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    687

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0746 [new locus tag: SA_RS04245 ]
  • symbol: SA0746
  • description: nuclease
  • length: 228
  • theoretical pI: 9.65007
  • theoretical MW: 25089.6
  • GRAVY: -0.324123

Function[edit source | edit]

  • reaction:
    EC 3.1.31.1?  ExPASy
    Micrococcal nucleaseEndonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
  • TIGRFAM:
    Signal transductionRegulatory functionsProtein interactionsPhr family secreted Rap phosphatase inhibitor (TIGR04429; HMM-score: 11.5)
  • TheSEED:  
    Phage-encoded chromosome degrading nuclease YokF 
  • PFAM:
    Tudor (CL0049) SNase; Staphylococcal nuclease homologue (PF00565; HMM-score: 122.1)
    no clan definedDUF2700; Protein of unknown function (DUF2700) (PF10912; HMM-score: 14.1)
    DUF1516; Protein of unknown function (DUF1516) (PF07457; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Ca2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.445
    • Ymax_pos: 61
    • Cmax: 0.293
    • Cmax_pos: 61
    • Smax: 0.877
    • Smax_pos: 52
    • Smean: 0.433
    • D: 0.44
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCLVLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENANKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNIWSEDNADSGQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]