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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0894 [new locus tag: SA_RS05075 ]
  • pan locus tag?: SAUPAN003242000
  • symbol: SA0894
  • pan gene symbol?: menA
  • synonym:
  • product: 1,4-dihydroxy-2-naphthoate octaprenyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0894 [new locus tag: SA_RS05075 ]
  • symbol: SA0894
  • product: 1,4-dihydroxy-2-naphthoate octaprenyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1014034..1014972
  • length: 939
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTAATCAATATCAGCAATATTCTACAGTTAAGAAATATTGGCATTTAATGCGTCCT
    CATACATTAACTGCTTCCGTAGTACCCGTTTTAGTTGGTACAGCAGCATCTAAAATATAT
    TTTCTTGGTAGCGAAGATCATATTAAAATCAGCCTATTCATTGCCATGTTACTAGCATGC
    TTACTTATTCAAGCAGCAACTAATATGTTTAATGAATACTATGATTATAAAAAAGGCCTT
    GATGATCATGAATCTGTAGGCATTGGTGGTGCCATTGTTCGCAACGGTATGAGCCCAGAG
    CTTGTGCTACGATTAGCCATTGCATTTTACATCTTAGCAGCAATATTAGGTTTGTTTTTA
    GCTGCTAACTCTTCATTTTGGTTATTACCAGTTGGATTAGTATGTATGGCTGTTGGTTAC
    CTATATACAGGTGGCCCTTTCCCTATTTCATGGACGCCTTTCGGTGAATTATTCTCAGGC
    GTATTTATGGGTATGTTTATTATCGTTATTGCATTCTTTATTCAAACTGGCAATATTCAA
    AGTTATGTAATTTGGTTAAGTGTACCTATAGTAATCACTATCGGTTTAATTAATATGGCT
    AACAATATTCGCGACCGTGTCAAAGATAAAGCAAGTGGTCGCAAAACTTTACCCATTCTA
    TTAGGTAAAAATGCTTCTTTAACATTTATGGCAATCATGTACTTTATCGCTTATGCCTTT
    ATTGTACTTACGATCATTATTAAACCTGGTGGCTCATTATTTTACTTACTTGCGTTGTTA
    TCATTCCCAATGCCTGTTAAAGTTATCAGACGTTTCAAGAAGAATGATACACCGCCTACA
    ATGATGCCAGCAATGGCTGCTGCTGGTAAAACAAATACATTTTTCGGTTTATTATATGCA
    TTAGGTATTTATATTAGTGCATTATTTGCAGGCATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    939

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0894 [new locus tag: SA_RS05075 ]
  • symbol: SA0894
  • description: 1,4-dihydroxy-2-naphthoate octaprenyltransferase
  • length: 312
  • theoretical pI: 9.91895
  • theoretical MW: 34400
  • GRAVY: 0.733654

Function[edit source | edit]

  • reaction:
    EC 2.5.1.74?  ExPASy
    1,4-dihydroxy-2-naphthoate polyprenyltransferaseAn all-trans-polyprenyl diphosphate + 1,4-dihydroxy-2-naphthoate = a demethylmenaquinol + diphosphate + CO2
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone1,4-dihydroxy-2-naphthoate octaprenyltransferase (TIGR00751; EC 2.5.1.74; HMM-score: 99.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone1,4-dihydroxy-2-naphthoate phytyltransferase (TIGR02235; EC 2.5.1.-; HMM-score: 98.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllchlorophyll synthase ChlG (TIGR02056; EC 2.5.1.62; HMM-score: 23.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneputative 4-hydroxybenzoate polyprenyltransferase (TIGR01475; EC 2.5.1.-; HMM-score: 21.5)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsQuinone cofactorsMenaquinone and Phylloquinone Biosynthesis 1,4-dihydroxy-2-naphthoate polyprenyltransferase (EC 2.5.1.74) 
    Quinone cofactorsMenaquinone biosynthesis from chorismate via 1,4-dihydroxy-2-naphthoate 1,4-dihydroxy-2-naphthoate polyprenyltransferase (EC 2.5.1.74) 
  • PFAM:
    Terp_synthase (CL0613) UbiA; UbiA prenyltransferase family (PF01040; HMM-score: 126.4)
    no clan definedUPF0767; UPF0767 family (PF15990; HMM-score: 10.6)
    DAGK_prokar; Prokaryotic diacylglycerol kinase (PF01219; HMM-score: 7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.224
    • Ymax_pos: 26
    • Cmax: 0.134
    • Cmax_pos: 38
    • Smax: 0.564
    • Smax_pos: 20
    • Smean: 0.389
    • D: 0.288
  • predicted transmembrane helices (TMHMM): 9

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGSEDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELVLRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSGVFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLPILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKNDTPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA0894 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]